Improved diagnosis of central venous catheter-related bloodstream infections using the HB&amp;L UROQUATTRO™ system

Fontana, Carla; Favaro, Marco; Bossa, Maria Cristina; Minelli, Silvia; Altieri, Anna; Pelliccioni, Marco; Falcione, F.; Traglia, Loide Di; Cicchetti, O.; Favalli, Cartesio

doi:10.1007/s10096-012-1676-9

Cited by 9 publications

(3 citation statements)

References 29 publications

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“…The instrument allows only for detection of live bacteria, because the initial blank value reading eliminates inert materials. The system was previously evaluated for microorganism enrichment, determination of microbial count, and diagnosis of central venous catheter-related bloodstream infections [ 18 , 19 ]. Alfred 60AST was initially conceived for urine screening and for AST of bacterial isolates from urine [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

A Prospective Evaluation of Two Rapid Phenotypical Antimicrobial Susceptibility Technologies for the Diagnostic Stewardship of Sepsis

Giordano

Piccoli

Brucculeri

et al. 2018

BioMed Research International

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Rapid identification of bloodstream pathogens by MALDI-TOF MS and the recently introduced rapid antimicrobial susceptibility testing (rAST) directly from positive blood cultures allow clinicians to promptly achieve a targeted therapy, especially for multidrug resistant microorganisms. In the present study, we propose a comparison between phenotypical rASTs performed in light-scattering technology (Alfred 60AST, Alifax®) and fluorescence in situ hybridization (Pheno™, Accelerate) directly from positive blood cultures, providing results in 4–7 hours. Blood samples from 67 patients admitted to the Azienda Ospedaliero-Universitaria Pisana were analyzed. After the direct MALDI-TOF MS identification, the rAST was performed at the same time both on Alfred 60AST and Pheno. Alfred 60AST provided qualitative results, interpreted in terms of clinical categories (SIR). Pheno provided identification and MIC values for each antibiotic tested. Results were compared to the broth microdilution assay (SensiTitre™, Thermo Fisher Scientific), according to EUCAST rules. Using Alfred 60AST, an agreement was reached, 91.1% for Gram-negative and 95.7% for Gram-positive bacteria, while using Pheno, the agreement was 90.6% for Gram-negative and 100% for Gram-positive bacteria. Both methods provided reliable results; Alfred 60AST combined with MALDI-TOF MS proved itself faster and cheaper. Pheno provided identification and MIC determination in a single test and, although more expensive, may be useful whenever MIC value is necessary and where MALDI-TOF MS is not present.

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Section: Introductionmentioning

confidence: 99%

A Prospective Evaluation of Two Rapid Phenotypical Antimicrobial Susceptibility Technologies for the Diagnostic Stewardship of Sepsis

Giordano

Piccoli

Brucculeri

et al. 2018

BioMed Research International

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“…Microbiological Approach in Diagnosing Native and Heart Valves Prosthesis Infections in a liquid media using HB&L Culture Kit vial (Alifax, Padova Italy) also supplemented with HB&L DEB Kit (Alifax) in order to guarantee the growth of the fastidious microorganism. Vials were incubated on SIDECAR automated system (Alifax) for 6 h. The SIDECAR is the automated version of HB&L together with the ALFRED system and it is also based on laser light scattering nephelometer technology [16,17]. At the end of the instrumental protocol, specimens were seeded onto a set of different media (all provided by bioMeriux; Marcy l'Etoile.…”

Section: Research Papermentioning

confidence: 99%

Microbiological Approach in Diagnosing Native and Heart Valves Prosthesis Infections

Fontana¹,

Favaro²,

Sordillo³

et al. 2017

Clinical-Investigation

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Background: Traditional culturing approaches either on native (NV) or on prosthetic valves (PV) are still not efficient in detecting pathogens responsible of infection. In fact, despite the continuous development of microbiological technologies, a truly valid technique that could be used as reference has yet to be found. Here we present a revised version of traditional culture methods based on a pre-treatment of both NVs and PVs by DL-dithiothreitol (DTT). Methods and findings: A total of 79 specimens were included in the study: 54 were NVs and 25 were PVs. We compared the results of both culturing methods and molecular assays performed on NVs/PVs collected in two different periods named pre-DTT and post-DTT, respectively. The protocol consisted in treating NV/PV by an appropriate volume of DTT, following which the suspension of bacteria/DTT was used for culture and molecular assay. In pre-DTT period five specimens were culture-positive and one was positive by molecular assay only (1/20; 5%), showing a culture positivity rate of 25% (5/20). In the post-DTT period, of 59 specimens processed, 19 were culture positive (19/59; 32%). Moreover, PCRs performed on specimens treated with DTT contributed to the identification of six additional positive specimens plus an identification of poly-microbial infection lost by culture (7/59; 12%). Conclusion: Our findings show that the use of DTT can be helpful in increasing the identification of microorganisms involved in NV/PV infections. Given its simple and cost-effective use and considering the issue that this technique does not require any specific instrumentation, it could easily be introduced in any laboratories. However, since our study included a limited number of specimens, more extensive studies are needed to further confirm our results.

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“…Since 1980s nephelometry based systems, which detect scattered light intensity changes in time due to microorganisms division, are used for direct determination of viable microorganisms in urine samples [6] . Uro-Quick (Alifax) and BacterioScan 216Dx (BacterioScan Inc.) systems allow not only to perform bacteriuria screening, but also to test bacterial cultures for antibiotic susceptibility [7] , [8] , [9] , [10] . For bacteriuria screening, the analyzed parameter is growth delay time after start of incubation: in the case of significant bacteriuria, fast growth is observed, in the case of contamination, growth is delayed for a few hours.…”

Section: Introductionmentioning

confidence: 99%

Coherent fluctuation nephelometry as a promising method for diagnosis of bacteriuria

Гурьев

Yudina

Lazareva

et al. 2018

Practical Laboratory Medicine

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ObjectivesSpecialized analyzers are used to automate the diagnosis of bacteriuria in laboratory practice. They are based on analysis of microorganisms concentration in urine samples or recording the growth of urine microflora. Coherent fluctuation nephelometry (CFN) has high sensitivity and allows analyzing both parameters simultaneously. The aim of the study is to compare the effectiveness of CFN-based and flow cytometry based analyzers.Design and methodsTotal 117 urine samples from children were studied in parallel using the CFN-analyzer and UF-1000i (Sysmex), the results were confirmed by conventional microbiological methods.ResultsIn 21 urine samples (18%), significant bacteriuria was determined (≥104 CFU/ml). The best diagnostic indicators were obtained while testing urine samples using the CFN-analyzer. The most efficient bacteriuria diagnosis is achieved by simultaneous analyses of microorganisms concentration in urine and growth of urine microflora (sensitivity – 95.2%, specificity – 96.9%, positive predictive value – 87%, negative predictive value – 98.9%, diagnostic odds ratio – 81.7, positive likelihood ratio – 30.5, negative likelihood ratio– 0.049, area under curve in ROC-analysis – 0.987). The CFN-analyzer allows the preliminary selection of negative urine samples, which do not require further analysis by conventional microbiological methods, thereby decreasing the number of cultures by 80.3%.ConclusionsThis study suggests that the CFN-analyzer is the effective tool for bacteriuria screening in children.

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Improved diagnosis of central venous catheter-related bloodstream infections using the HB&L UROQUATTRO™ system

Cited by 9 publications

References 29 publications

A Prospective Evaluation of Two Rapid Phenotypical Antimicrobial Susceptibility Technologies for the Diagnostic Stewardship of Sepsis

A Prospective Evaluation of Two Rapid Phenotypical Antimicrobial Susceptibility Technologies for the Diagnostic Stewardship of Sepsis

Microbiological Approach in Diagnosing Native and Heart Valves Prosthesis Infections

Coherent fluctuation nephelometry as a promising method for diagnosis of bacteriuria

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