Improved fertilization and implantation rates after non-touch zona pellucida microdrilling of mouse oocytes with a 1.48  m diode laser beam

Germond, Marc; Nocera, Dorotha; Senn, A.; Rink, Klaus; Delacréaz, G.; Pedrazzini, Thierry; Hornung, Jean‐Pierre

doi:10.1093/oxfordjournals.humrep.a019294

Cited by 63 publications

(46 citation statements)

References 32 publications

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“…Mouse Oocytes and Zygotes-6 to 9-week-old IB1/JIP-1 heterozygous females were stimulated (day 1) with one peritoneal injection (10 IU/0.2ml) of follicle-stimulating hormone (Folligon; Intervet AG, Pfä ffikon, Switzerland) followed on day 3 by a second injection (10 IU/0.2ml) of hCG (Pregnyl; Organon, Zü rich, Switzerland) to induce ovulation (26). For the recovery of oocytes, females were killed 13 h after hCG administration by cervical dislocation.…”

Section: Generationmentioning

confidence: 99%

Islet-Brain1/JNK-interacting Protein-1 Is Required for Early Embryogenesis in Mice

Thompson¹,

Haefliger²,

Senn³

et al. 2001

Journal of Biological Chemistry

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Islet-brain1/JNK-interacting protein-1 (IB1/JIP-1) is a scaffold protein that organizes the JNK, MKK7, and MLK1 to allow signaling specificity. Targeted disruption of the gene MAPK8IP1 encoding IB1/JIP-1 in mice led to embryonic death prior to blastocyst implantation. In culture, no IB1/JIP-1 ؊/؊ embryos were identified indicating that accelerated cell death occurred during the first cell cycles. IB1/JIP-1 expression was detected in unfertilized oocytes, in spermatozoa, and in different stages of embryo development. Thus, despite the maternal and paternal transmission of the IB1/JIP-1 protein, early transcription of the MAPK8IP1 gene is required for the survival of the fertilized oocytes.

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Section: Generationmentioning

confidence: 99%

Islet-Brain1/JNK-interacting Protein-1 Is Required for Early Embryogenesis in Mice

Thompson¹,

Haefliger²,

Senn³

et al. 2001

Journal of Biological Chemistry

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“…As laser beams may now be guided through optical lenses, it is possible to exactly focus the laser beam to less than 1 Ìm in diameter. In assisted reproductive technologies, laser light was used for manipulation of sperm and oocytes, thus supporting or replacing conventional manipulation methods [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Laser Microdissection as a New Approach to Prefertilization Genetic Diagnosis

Clement-Sengewald

Buchholz²,

Schütze³

2000

Pathobiology

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The genetic status of oocytes can be determined by polar body (PB) analysis. Following PB extraction, a genetic evaluation is performed. As each PB contains the complementary genetic material of the oocyte, PB analysis reveals information about its genetic status. Genetically altered oocytes may then be excluded from in vitro fertilization. The aim of our study was to evaluate laser microdissection as a tool for PB extraction purposes. Compared to the PB extraction with a sharp-ending pipette only, we could show that laser microdissection of the zona pellucida (laser zona drilling) with a UV-A laser and subsequent extraction with a blunt-ending pipette decreases the degeneration rate of oocytes. It is shown that laser pressure catapulting of extracted PB enables their contact-free transfer into tubes, thus decreasing the risk of contamination for further analysis.

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“…The procedure seems to be safe, as drilled mouse embryos give rise to normal and fertile offspring. Pups derived from zona pellucida microdrilled embryos were comparable with those obtained from control embryos, confirming the lack of deleterious effects from the laser treatment [Germond et al, 1996]. It is concluded that the technology may be investigated in humans, particularly when the zona pellucida represents the main impediment to fertilization or assisted hatching, but clinical studies have not been reported so far.…”

Section: Animal Studies and In Vitro Human Embryo Hatchingmentioning

confidence: 60%

“…However, infrared radiation is the most appropriate, especially when mutagenic risks are taken into account. The 1.48-Ìm diode laser allows rapid, precise and easily controlled lysis of the zona pellucida [Rink et al, 1994;Germond et al, 1995Germond et al, , 1996. Zona opening is performed by exposing the zona pellucida to laser light.…”

Section: Different Techniques For Assisted Hatchingmentioning

confidence: 99%

Zona Hardening, Zona Drilling and Assisted Hatching: New Achievements in Assisted Reproduction

Vos¹,

Steirteghem²

2000

Cells Tissues Organs

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Prior to fertilization, the zona pellucida surrounding the mammalian oocyte acts as a species-specific sperm barrier and is involved in sperm binding. After fertilization, the zona plays a role in blocking polyspermic fertilization, it protects the integrity of the preimplantation embryo during early embryonic development, and also helps its oviductal transport. Zona hardening occurs naturally after fertilization in order to ensure this threefold function. A combination of lysins produced by the cleaving embryo or the uterus and physical expansion then reduces the zona thickness in preparation for hatching. Zona hardening, although not readily quantifiable, may also be induced by in vitro culture and by in vivo aging. Indeed, prolonged exposure of human oocytes and embryos to artificial culture conditions seems to impair their ability to implant. Implantation rates are also inversely correlated with advanced female age. Recently, failure of the embryonic zona pellucida to rupture following blastocyst expansion has been put forward as a possible contributing factor in implantation failure. In order to help embryos escape from their zonae during blastocyst expansion, different types of assisted hatching have been developed. Zona drilling involves the creation of an opening in the zona with acidified medium, whereas zona slitting is carried out in the same manner as partial zona dissection. In zona thinning, the zona is just made thinner over a certain area without a hole or a slit being created. More recently, laser-assisted hatching has been introduced. In vitro studies with both mouse and human embryos have indicated that an artificial gap in the zona pellucida significantly improves the hatching ability of blastocysts grown in vitro as compared to non-micromanipulated embryos. However, the clinical relevance of assisted hatching within an assisted reproduction program remains controversial and elusive. Very few randomized studies are available. Most reports are of retrospective analyses which report either no differences in implantation and pregnancy rates between assisted hatching and control embryos or better results after assisted hatching. Five randomized controlled studies suggest that assisted hatching – of no benefit to the overall patient population – might be of value in increasing embryo implantation rates only in selected cases. No further evidence exists for an age-related benefit from assisted hatching in patients with advanced maternal age.

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Improved fertilization and implantation rates after non-touch zona pellucida microdrilling of mouse oocytes with a 1.48 m diode laser beam

Cited by 63 publications

References 32 publications

Islet-Brain1/JNK-interacting Protein-1 Is Required for Early Embryogenesis in Mice

Islet-Brain1/JNK-interacting Protein-1 Is Required for Early Embryogenesis in Mice

Laser Microdissection as a New Approach to Prefertilization Genetic Diagnosis

Zona Hardening, Zona Drilling and Assisted Hatching: New Achievements in Assisted Reproduction

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