Improved hydrogen production by uptake hydrogenase deficient mutant strain of Rhodobacter sphaeroides O.U.001

Best mode for photo-fermentation hydrogen production: The semi-continuous operation

“…This also indicated that high yield and rate of hydrogen production resulted in low biomass. The result was consistent with Kars's report about biomass in hydrogen production was lower than that in no producing hydrogen [16].…”

Microbiological Methods of Hydrogen Generation

“…The engineering of the mutants free of uptake hydrogenase is the basic task of gene modifications. Genes coding hydrogenase (hup) can be modified by resistance gene insertion into the hup genes or by deletion of hup genes (Kars, 2009, Kars, 2008. Appropriately modified Rhodobacter spheroids can generate hydrogen also in the absence of light (Kim, 2008).…”

Transferring [NiFe] hydrogenase gene from Rhodopeseudomonas palustris into E. coli BL21(DE3) for improving hydrogen production

“…G€ okhan Kars [14] and I.-H. Lee [15] successfully increased hydrogen productivity by 20% and 43% through knocking-out hupSL gene, respectively. Moreover, Elisabetta Franchi [16] and Yavuz € Oztü rk [17] also increased hydrogen productivity by 30% and 29% through knocking-out hupSL gene or hupSLC gene, respectively.…”