Improved immunogenicity and protective efficacy of a divalent DNA vaccine encoding Brucella L7/L12-truncated Omp31 fusion protein by a DNA priming and protein boosting regimen

Golshani, Maryam; Rafati, Sima; Siadat, Seyed Davar; Nejati-Moheimani, Mehdi; Shahcheraghi, Fereshteh; Arsang, Amin

doi:10.1016/j.molimm.2015.04.015

Cited by 29 publications

(14 citation statements)

References 32 publications

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“…Similarly, mice immunized with the DNA priming and protein boosting vaccination regimen of Brucella outer membrane protein 31 and L7/L12 exhibited significantly enhanced antigen‐specific antibody titre, IFN‐γ production in splenocytes and resistance to challenge with live Brucella pathogens (Golshani et al . ).…”

Section: Discussionmentioning

confidence: 97%

A DNA priming and protein boosting immunization scheme to augment immune responses against parvovirus in ducks

Lee

Lin

et al. 2018

J Appl Microbiol

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Aims:To evaluate the effect of a DNA priming and protein boosting immunization scheme in ducks. Methods and results: Pekin ducks were immunized with pTCY/VP2 DNA vaccine; on day 14 (D14) after primary immunization, the ducks were boosted with either the same vaccine (DNA + DNA) or the rVP2 vaccine (DNA + rVP2). CpG oligodeoxynucleotides containing three copies of GACGTT motifs were used as the adjuvant in the vaccines. Compared with unimmunized controls, both immunization schemes significantly increased the titre of antigen-specific antibodies, lymphocyte proliferation index, percentage of CD4 + and CD8 + cells in peripheral blood mononuclear cells (PBMCs) and mRNA expression of interferon (IFN)-a, IFN-c, interleukin (IL)-6 and IL-12 in antigen-stimulated PBMCs. Furthermore, compared with the DNA + DNA homologous scheme, the DNA + rVP2 heterologous scheme significantly increased lymphocyte proliferation, percentage of CD4 + and CD8 + cells in PBMCs and upregulation of mRNA expression of cytokines 2 weeks after the boost (D28). Conclusions: The DNA + rVP2 immunization scheme enhanced immune responses, mainly Th1 type, against parvovirus in ducks. Significance and Impact of the Study: The DNA priming and protein boosting heterologous immunization strategy can be applied to develop vaccines against viral infections in ducks. It can potentially be used in breeding ducks because of long-term immunity may confer protection for ducklings.

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Section: Discussionmentioning

confidence: 97%

A DNA priming and protein boosting immunization scheme to augment immune responses against parvovirus in ducks

Lee

Lin

et al. 2018

J Appl Microbiol

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“…Specific antibodies are important in reducing the initial phase of a Brucella infection, but a strong humoral immunity unaccompanied by cell-mediated immunity (CMI) cannot provide total protection against the Brucella organism (Lalsiamthara and Lee, 2017). In some situations, inoculation with "naked" plasmid DNA has been shown to not produce an immune response as strong as either purified protein or even a commercial Brucella vaccine (Cassataro et al, 2007;Golshani et al, 2015). DNA vaccines may be less potent than live attenuated vaccines because DNA is not as evenly distributed among initially transfected cells, while the number of cells infected with the live attenuated microorganism increases as it replicates (Kano et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…It is considered a strategy capable of inducing a type 1 immune response (Th1) associated with IFN-γ producing CD4 + T cells, CD8 + cytotoxic T cells and IgG2a antibodies produced by B cells, all of which are necessary to eliminate infection by B. abortus (Golding et al, 2001). In addition, it is possible to enhance DNA vaccine immune responses by using a DNA prime-protein boost (Cassataro et al, 2007;Golshani et al, 2015). An initial immunization with DNA, followed by enhancement with recombinant VirB genes vaccines against Brucella abortus s3 protein, can increase the efficacy of a vaccine against Brucella infection (Cassataro et al, 2007;Golshani et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, it is possible to enhance DNA vaccine immune responses by using a DNA prime-protein boost (Cassataro et al, 2007;Golshani et al, 2015). An initial immunization with DNA, followed by enhancement with recombinant VirB genes vaccines against Brucella abortus s3 protein, can increase the efficacy of a vaccine against Brucella infection (Cassataro et al, 2007;Golshani et al, 2015). One of the main virulence factors that mediates intracellular survival of different species of Brucella is the type IV secretion system (T4SS), encoded by the virB operon (virB1 to virB12).…”

Section: Introductionmentioning

confidence: 99%

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Research Article DNA vaccine and DNA prime-protein boost with the virB9 and virB12 genes induced low level of protection against Brucella abortus infection in mice

Caitano-Bertolacci¹,

Bastos²,

Santos³

et al. 2019

Genet. Mol. Res.

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VIRB proteins from Brucella spp. constitute the type IV Secretion System (T4SS), a key virulence factor that mediates the intracellular survival of these bacteria. We investigated the immunogenicity and protection of proteins produced by the virB9 and virB12 genes in the DNA vaccine and DNA prime-protein boost strategies. Groups of 10 mice were vaccinated with pcDNAvirB9, pcDNAvirB12, pcDNAvirB9+rVIRB9 or pcDNAvirB12+rVIRB12. The latter two groups were vaccinated with the proteins rVIRB9 and rVIRB12, respectively, during the third immunization. Three weeks after the last immunization, six animals from each group were challenged intraperitoneally with B. abortus strain S2308, and the efficacy of the vaccines was calculated as the log 10 of protection by subtracting the mean log CFU of the vaccinated group from the mean log CFU of the ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 18 (2): gmr18295 M.A.B. Caitano-Bertolacciet al. 2negative control group (injected with sterile saline). Most of the vaccinated mice produced total IgG and the subclasses IgG1 and IgG2a against the respective protein, except for the mice vaccinated with pcDNAvirB12. Cytokines IFN-γ and IL-10 were produced, but without a significant difference between the vaccinated and negative control groups. The vaccines did not induce significant levels of protection, in contrast to the immunization obtained with the S19 vaccine strain (Log 10 , 1.48). In conclusion, the virB9 and virB12 genes of B. abortus, using DNA vaccine and DNA prime-protein boost strategies, were able to induce both humoral and cellular immune responses, but not enough to induce significant protection in the immunized mice. However, given the response in this system, further investigations using the virB9 and virB12 genes of Brucella spp., together with different immune modulators, are warranted. An effort should be made to direct and enhance the immune response, in order to identify a combination that stimulates a better immune response and, consequently, a better level of protection.

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“…The Rose Bengal plate test (RBPT), plate agglutination test (PAT), standard tube agglutination test (SAT), complement fixation test (CFT), Coombs anti-Brucella test and enzyme-linked immunosorbent assay (ELISA) are considered to be the main methods for serological diagnosis of brucellosis disease [6][7][8]. However, these methods usually use the whole cell or smooth lipopolysaccharides (S-LPS) as the antigen to detect Bru- cella antibodies in the patient's serum, which can lead to false positives and cross-reactivity with other Gram-negative bacteria such as Yersinia entercolitica O:9 [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

A sandwich immunoassay for brucellosis diagnosis based on immune magnetic beads and quantum dots

Yin

et al. 2017

Journal of Pharmaceutical and Biomedical Analysis

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Improved immunogenicity and protective efficacy of a divalent DNA vaccine encoding Brucella L7/L12-truncated Omp31 fusion protein by a DNA priming and protein boosting regimen

Cited by 29 publications

References 32 publications

A DNA priming and protein boosting immunization scheme to augment immune responses against parvovirus in ducks

A DNA priming and protein boosting immunization scheme to augment immune responses against parvovirus in ducks

Research Article DNA vaccine and DNA prime-protein boost with the <i>virB9</i> and <i>virB12</i> genes induced low level of protection against <i>Brucella</i> <i>abortus</i> infection in mice

A sandwich immunoassay for brucellosis diagnosis based on immune magnetic beads and quantum dots

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