Improved in vitro micropropagation method with adventitious corms and roots for endangered saffron

Zeybek, Evrim; Önde, Sertaç; Kaya, Zeki

doi:10.2478/s11535-011-0102-0

Cited by 18 publications

(12 citation statements)

References 21 publications

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“…MS medium containing BA (13.32 µM L -1 ) with 2,4-D (0.90 µM L -1 ) produced 100% callus response in S. chirayita from root explants after five weeks of culture [22]. Similarly, in saffron least concentration of 2,4-D (0.25 mg L -1 ) in combination with and BA (1 mg L -1 ) was found to be best for callus initiation [23]. In several other Gentianaceae species like Swertia angustifolia and Swertia mussotti higher concentration of 2,4-D (3.0 mg L -1 ) and Kn (2.5 mg L -1 ) exhibited best callusing in leaf explants [12,13].…”

Section: Resultsmentioning

confidence: 99%

In vitro organogenesis secondary metabolite production and heavy metal analysis in Swertia chirayita

et al. 2014

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An efficient protocol of plant regeneration through direct and indirect organogenesis in Swertia chirayita was developed. Explants cultured on Murashige and Skoog medium supplemented with 2,4-D (0.5 mg L−1) with combination of Kinetin (0.5 mg L−1) showed the highest frequency (84%) of callusing and 1.0mg L−1 6-benzyladenine (BA) in combination with (100 mg L−1) Adenine sulphate (Ads) + (0.1 mg L−1) Indole acetic acid (IAA) was excellent for maximum adventitious shoot (12.69 ± 1.30) formation in four week of culture. A maximum number of (7.14 ± 0.99) shoots were developed per leaf explants through direct organogenesis. The highest frequency of rooting (11.46 ± 1.56) was observed on MS medium augmented with IAA (1.0 mg L−1). Well-rooted shoots transferred to plastic pots containing a soilrite: sand mix and then moved to the greenhouse for further growth and development. Four major secondary metabolites were analyzed and quantified using high performance liquid chromatography. Amount of secondary metabolites was found significantly higher, in in vitro plantlets compared to in vivo plantlets and callus raised from S. chirayita. Higher heavy metal accumulation in in vitro as compared to in vivo plantlets correlates higher secondary metabolite production supporting that they play regulatory role in influencing the plant secondary metabolism.

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Section: Resultsmentioning

confidence: 99%

In vitro organogenesis secondary metabolite production and heavy metal analysis in Swertia chirayita

et al. 2014

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“…In addition, the vigorous roots are also generated in ½MS medium supplemented with 0.5 mg dm -3 IBA (Zang et al 2017). However, high induction rate of adventitious roots could be achieved by using MS media supplemented with 2.0 mg dm -3 IBA in an efficient in vitro tissue culture system for Crocus sativus (Zeybek et al 2012). Therefore, different types of medium (MS or ½MS) played distinct roles in plant rooting.…”

Section: Discussionmentioning

confidence: 99%

Rapid and efficient leaf regeneration propagation system for Euonymus bungeanus

Kang¹,

Zhou²,

Jiang³

et al. 2021

Biologia plant.

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Rapid propagation of plants by tissue culture is of great significance for large-scale production, molecular genetics research, and breeding. Currently, a rapid and high-efficient tissue culture protocol for Euonymus bungeanus is needed. To develop a propagation system for this species, we established a new regeneration system from leaves. Callus formation was induced on Murashige and Skoog (MS) medium supplemented with 0.5 mg dm -3 6-benzylaminopurine (6-BA) and 0.5 mg dm -3 α-naphthalene acetic acid (NAA) and the induction rate almost reached 100 % under red radiation within 21 d. The medium for proliferation of adventitious buds comprised of MS medium with 1.0 mg dm -3 6-BA and 0.5 mg dm -3 NAA, and the induction rate within 20 d nearly reached 100 %. When, the adventitious buds were transferred to the rooting medium containing ½MS, 2.0 mg dm -3 indole-3-butyric acid (IBA), and 0.05 mg dm -3 NAA, adventitious root formation was achieved within 20 d. Collectively, the rapid and high-efficient regeneration system from E. bungeanus leaves was established, providing useful references for effective mass propagation and it could serve as an enabling technology for future genetic engineering.

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“…Besides, cormlet development in Crocus tissue cultures occurs under various PGR treatments. Several papers report the importance of IBA (Zeybek et al, 2012), while others underline the use of (Sharma et al, 2008) for C. sativus. The use of high sucrose concentration and high cytokinin/auxin ratios was proven to be beneficial for plant regeneration and cormlet production from corm-derived somatic embryos in C. vernus (Sivanesan et al, 2012(Sivanesan et al, , 2014.…”

Section: Discussionmentioning

confidence: 99%

Production and Characterization of Tissue Cultures of Four Crocus Species from the Carpathian Basin

Freytag¹,

Pabar²,

Demeter³

et al. 2017

Acta Biologica Cracoviensia S. Botanica

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We aimed to produce tissue cultures and plant regeneration from endangered Crocus species: C. scepusiensis, C. tommasinianus, C. vittatus ("Verni" series of the genus) and C. banaticus. For initiation of cultures we used a plant growth regulator (PGR) combination used for in vitro culture of saffron and its relatives: 10 mg L -1 α-naphthaleneacetic acid (NAA) and 1 mg L -1 6-benzyladenine (BA). Shoot tips of young seedlings (C. scepusiensis) and corms (for the rest of species) were used as explants. C. scepusiensis explants developed into organogenic calli. On media with decreased NAA and with or without increased BA concentration, calli produced stigma-like structures and/or shoots and whole plants. In the other species, callus initiation medium induced callus formation with abundant somatic embryos. In C. tommasinianus, embryos developed shoots when auxin content of medium was decreased. In C. banaticus, a decrease of auxin with or without an increase in cytokinin content led to shoot or whole plant regeneration, as in C. scepusiensis. In the case of C. vittatus and C. banaticus, initiation and/or maintenance of cultures on indole-3-butyric acid (IBA) and increased sucrose concentration stimulated whole plant regeneration and in vitro cormlet development. C. scepusiensis and the rest of cultures (organogenic vs. embryogenic) differed at the biochemical level: C. scepusiensis cultures had higher (yet still low) enzymatic antioxidant (catalase, peroxidase) activities. With respect to catalase isoenzyme patterns, C. banaticus was different from the rest of cultures, demonstrating its distinct taxonomical position. Besides germplasm preservation use of the present cultures, they have a potential biotechnological value.

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Improved in vitro micropropagation method with adventitious corms and roots for endangered saffron

Cited by 18 publications

References 21 publications

In vitro organogenesis secondary metabolite production and heavy metal analysis in Swertia chirayita

In vitro organogenesis secondary metabolite production and heavy metal analysis in Swertia chirayita

Rapid and efficient leaf regeneration propagation system for Euonymus bungeanus

Production and Characterization of Tissue Cultures of Four Crocus Species from the Carpathian Basin

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