Improved method for optical fiber temperature probe implantation in brains of free-moving rats

Musolino, Stefan; Schartner, Erik P.; Hutchinson, Mark R.; Salem, Abdallah

doi:10.1016/j.jneumeth.2018.12.013

Cited by 14 publications

(4 citation statements)

References 27 publications

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“…The optical fiber was inserted into specific regions of the brain, with the tip positioned at a distance of 0 from the target area. 6 , 20 In order to ensure the optical fiber’s safety, the dental cement was utilized to secure it, while a sleeve served the purpose of protecting it. The mice stayed on the warming mat until they completely regained consciousness and were administered Baytril (10 mg/kg, s.c.) twice a day for 5 days.…”

Section: Methodsmentioning

confidence: 99%

Low-frequency electroacupuncture exerts antinociceptive effects through activation of POMC neural circuit induced endorphinergic input to the periaqueductal gray from the arcuate nucleus

Wang,

Li,

Nie

et al. 2024

Mol Pain

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It has been widely recognized that electroacupuncture (EA) inducing the release of β-endorphin represents a crucial mechanism of EA analgesia. The ARC is a vital component of the endogenous opioid peptide system. However, the specific mechanisms how EA facilitates the release of β-endorphin within the ARC, eliciting analgesic effects are yet to be elucidated. In this study, we conducted in vivo and in vitro experiments by transcriptomics, microdialysis, photogenetics, chemical genetics, and calcium imaging, combined with transgenic animals. Firstly, we detected 2Hz EA at the Zusanli (ST36) increased the level of β-endorphin and transcriptional level of POMC. Our transcriptomics profiling demonstrated that 2Hz EA at the ST36 modulates the expression of c-Fos and Jun B in ARC brain nuclear cluster, and the transcriptional regulation of 2Hz EA mainly occur in POMC neurons by immunofluorescence staining verification. Meaning while, 2Hz EA specifically activated the cAMP-PKA-CREB signaling pathway in ARC which mediating the c-Fos and Jun B transcription, and 2Hz EA analgesia is dependent on the activation of cAMP-PKA-CREB signaling pathway in ARC. In order to investigate how the β-endorphin produced in ARC transfer to integration center PAG, transneuronal tracing technology was used to observe the 2Hz EA promoted the neural projection from ARC to PAG compared to 100Hz EA and sham mice. Inhibited PAGGABA neurons, the transfer of β-endorphin from the ARC nucleus to the PAG nucleus through the ARCPOMC-PAGGABA neural circuit. Furthermore, by manipulating the excitability of POMC neurons from ARCPOMC to PAGGABA using inhibitory chemogenetics and optogenetics, we found that this inhibition significantly reduced transfer of β-endorphin from the ARC nucleus to the PAG nucleus and the effectiveness of 2Hz EA analgesia in neurological POMC Cre mice and C57BL/6J mice, which indicates that the transfer of β-endorphin depends on the activation of POMC neurons prefect from ARCPOMC to PAGGABA.

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Section: Methodsmentioning

confidence: 99%

Low-frequency electroacupuncture exerts antinociceptive effects through activation of POMC neural circuit induced endorphinergic input to the periaqueductal gray from the arcuate nucleus

Wang,

Li,

Nie

et al. 2024

Mol Pain

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“…As a consequence, conventional infrared thermometry (fast and inexpensive) is not valid for brain temperature imaging as it cannot provide subcutaneous thermal readings (as in the case of the example shown in Figure 3 ). Instead, brain temperature has been measured so far using two technologies: - Intracranial implantation of thermal sensors (thermocouples or optical fibres) ( Musolino et al, 2019 ). This approach provides absolute and reliable thermal readouts without needing animal immobilization, but requiring skull piercing that, as mentioned earlier, is accompanied by several side effects.…”

Section: Monitoring Brain Activity Through Temperature: Fundamentals ...mentioning

confidence: 99%

“…- Intracranial implantation of thermal sensors (thermocouples or optical fibres) ( Musolino et al, 2019 ). This approach provides absolute and reliable thermal readouts without needing animal immobilization, but requiring skull piercing that, as mentioned earlier, is accompanied by several side effects.…”

Section: Monitoring Brain Activity Through Temperature: Fundamentals ...mentioning

confidence: 99%

Luminescence Thermometry for Brain Activity Monitoring: A Perspective

et al. 2022

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Minimally invasive monitoring of brain activity is essential not only to gain understanding on the working principles of the brain, but also for the development of new diagnostic tools. In this perspective we describe how brain thermometry could be an alternative to conventional methods (e.g., magnetic resonance or nuclear medicine) for the acquisition of thermal images of the brain with enough spatial and temperature resolution to track brain activity in minimally perturbed animals. We focus on the latest advances in transcranial luminescence thermometry introducing a critical discussion on its advantages and shortcomings. We also anticipate the main challenges that the application of luminescent nanoparticles for brain thermometry will face in next years. With this work we aim to promote the development of near infrared luminescence for brain activity monitoring, which could also benefit other research areas dealing with the brain and its illnesses.

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“…[ 5 ] Optical temperature sensors, such as infrared spectroscopy and optical fibers employing Bragg gratings or doping rare earth ions, are immune to electromagnetic noise, which can be employed to detect tissue temperature. [ 6 , 7 ] But these approaches are limited to point measurements at low spatial resolutions, failing in measuring brain temperature distribution. [ 8 ] Magnetic resonance spectroscopy (MRS) can be used to detect small temperature variations (<1 °C) in the brains, however, the high cost of MRS instruments and measurement artifacts become crucial barriers for practical measurements.…”

Section: Introductionmentioning

confidence: 99%

Upconversion‐Luminescent Fiber Microchannel Sensors for Temperature Monitoring at High Spatial Resolution in the Brains of Freely Moving Animals

Zhou,

Fan,

Zhai

et al. 2023

Advanced Science

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Brain temperature is a critical factor affecting neural activity and function, whose fluctuations may result in acute life‐threatening health complications and chronic neuropathology. To monitor brain temperature, luminescent nanothermometry (LN) based on upconversion nanoparticles (UCNPs) with low autofluorescence has received extensive attention for its advantages in high temperature sensitivity and high response speed. However, most of current the LNs are based on optical imaging, which fails in temperature monitoring in deep brain regions at high spatial resolution. Here, the fiber microchannel sensor (FMS) loaded with UCNPs (UCNP‐FMS) is presented for temperature monitoring at high spatial resolution in the deep brains of freely moving animals. The UCNP‐FMS is fabricated by incorporating UCNPs in microchannels of optical fibers, whose diameter is ∼50 µm processed by femtosecond laser micromachining for spatially resolved sensing. The UCNPs provide thermal‐sensitive upconversion emissions at dual wavelengths for ratiometric temperature sensing, ensuring a detection accuracy of ± 0.3 °C at 37 °C. Superior performances of UCNP‐FMS are demonstrated by real‐time temperature monitoring in different brain regions of freely moving animals under various conditions such as taking food, undergoing anesthesia/wakefulness, and suffering external temperature changes. Moreover, this study shows the capability of UCNP‐FMS in distributed temperature sensing in mammalian brains in vivo.

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Improved method for optical fiber temperature probe implantation in brains of free-moving rats

Cited by 14 publications

References 27 publications

Low-frequency electroacupuncture exerts antinociceptive effects through activation of POMC neural circuit induced endorphinergic input to the periaqueductal gray from the arcuate nucleus

Low-frequency electroacupuncture exerts antinociceptive effects through activation of POMC neural circuit induced endorphinergic input to the periaqueductal gray from the arcuate nucleus

Luminescence Thermometry for Brain Activity Monitoring: A Perspective

Upconversion‐Luminescent Fiber Microchannel Sensors for Temperature Monitoring at High Spatial Resolution in the Brains of Freely Moving Animals

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