Improved nitrogen use efficiency in transgenic sugarcane: phenotypic assessment in a pot trial under low nitrogen conditions

Snyman, S. J.; Hajari, Elliosha; Watt, M. P.; Lu, Yingzhi; Kridl, Jean C.

doi:10.1007/s00299-015-1768-y

Cited by 29 publications

(17 citation statements)

References 5 publications

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“…The overexpression of barley AlaAT in rice, which was also driven using a root-specific promoter ( OsANT1 ), also resulted in increased biomass and grain yield 44 . Recently, similar results have been reported for sugarcane 45 .…”

Section: The Two Most Limiting Nutrients For Crop Productivity: Phospsupporting

confidence: 87%

Engineering food crops to grow in harsh environments

et al. 2015

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Achieving sustainable agriculture and producing enough food for the increasing global population will require effective strategies to cope with harsh environments such as water and nutrient stress, high temperatures and compacted soils with high impedance that drastically reduce crop yield. Recent advances in the understanding of the molecular, cellular and epigenetic mechanisms that orchestrate plant responses to abiotic stress will serve as the platform to engineer improved crop plants with better designed root system architecture and optimized metabolism to enhance water and nutrients uptake and use efficiency and/or soil penetration. In this review we discuss such advances and how the generated knowledge could be used to integrate effective strategies to engineer crops by gene transfer or genome editing technologies.

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Section: The Two Most Limiting Nutrients For Crop Productivity: Phospsupporting

confidence: 87%

Engineering food crops to grow in harsh environments

et al. 2015

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“…The nitrogen use efficiency (NUE, crop production/fertilizer N supplied) of sugarcane genotypes varied greatly, and high NUE varieties are an important issue for sugarcane industry. One avenue helps to alleviate the excessive use of N is to determine the NUE of crops based on the various absorption and utilization characteristics of N-fertilizer and then the appropriate amount of N-fertilizer was applied (Hirel et al, 2001; Snyman et al, 2015). Therefore, the identification of NUE types of crops for rational N fertilizer application and how to identify a variety with high NUE under low N conditions are of great significance for reducing N pollution.…”

Section: Introductionmentioning

confidence: 99%

The Physiological and Agronomic Responses to Nitrogen Dosage in Different Sugarcane Varieties

et al. 2019

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Nitrogen (N) is very important for sugarcane yield improvement, but the excessive application of N fertilizer brings about N pollution and a cost increase. Through distinguishing the difference of nitrogen use efficiency (NUE), we can reasonably apply N fertilizer according to the NUE characteristics of sugarcane varieties, and thus reduce N loss and maintain high yield. The present study showed the pot experiment results of identifying NUE types of nine main sugarcane varieties in the main sugarcane producing areas of China under controlled conditions, and identified the key physiological and agronomic indictors which can help to determine the NUE types of sugarcane. The test clones were exposed to varying levels of N fertilizer and 15 parameters that are likely to impact NUE were measured. The key results are (1) Sugarcane variety ROC22 has the high plant dry weight (PDW) and NUE among nine varieties under different N rates, it can take advantages under low N supply (225 kg/hm 2 urea), and less N fertilizer can be applied properly in production. (2) Varieties of GT32 was good performing genotype for PDW and NUE under low N supply (225 kg/hm 2 urea), GT42 was more suitable for moderate N environment (450 kg/hm 2 urea), while YT94-128 was at middle N and high N supply (450–675 kg/hm 2 urea). (3) Late stage of shoot elongation is suitable for differentiating sugarcane clones for NUE. (4) Leaf glutamine synthetase activity is the most reliable predictor of NUE in sugarcane. The result of pot experiment is sufficient to differentiate clonal variation for NUE in sugarcane as it reflects field experimental results. This study can set up a basis for identification the NUE types of sugarcane varieties and the development of reasonable N fertilizer application.

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“…The transcriptome of transgenic rice roots and shoots over-expressing alanine aminotransferase under the control of a tissue-specific promoter was not significantly different from that of controls33. Sugarcane lines transformed with barley alanine aminotransferase gene driven by rice aldehyde dehydrogenase gene ( OsAnt1 ) promoter showed improved N use efficiency compared with untransformed ones in a pot trial under low nitrogen conditions34. Recent studies have indicated that the over-expression of AlaAT variants other than barley AlaAT in A. thaliana could further increase the N use efficiency phenotype(s)35.…”

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confidence: 98%

Identification and expression analyses of the alanine aminotransferase (AlaAT) gene family in poplar seedlings

Zhang

et al. 2017

Sci Rep

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Alanine aminotransferase (AlaAT, E.C.2.6.1.2) catalyzes the reversible conversion of pyruvate and glutamate to alanine and α-oxoglutarate. The AlaAT gene family has been well studied in some herbaceous plants, but has not been well characterized in woody plants. In this study, we identified four alanine aminotransferase homologues in Populus trichocarpa, which could be classified into two subgroups, A and B. AlaAT3 and AlaAT4 in subgroup A encode AlaAT, while AlaAT1 and AlaAT2 in subgroup B encode glutamate:glyoxylate aminotransferase (GGAT), which catalyzes the reaction of glutamate and glyoxylate to α-oxoglutarate and glycine. Four AlaAT genes were cloned from P. simonii × P. nigra. PnAlaAT1 and PnAlaAT2 were expressed predominantly in leaves and induced by exogenous nitrogen and exhibited a diurnal fluctuation in leaves, but was inhibited in roots. PnAlaAT3 and PnAlaAT4 were mainly expressed in roots, stems and leaves, and was induced by exogenous nitrogen. The expression of PnAlaAT3 gene could be regulated by glutamine or its related metabolites in roots. Our results suggest that PnAlaAT3 gene may play an important role in nitrogen metabolism and is regulated by glutamine or its related metabolites in the roots of P. simonii × P. nigra.

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Improved nitrogen use efficiency in transgenic sugarcane: phenotypic assessment in a pot trial under low nitrogen conditions

Cited by 29 publications

References 5 publications

Engineering food crops to grow in harsh environments

Engineering food crops to grow in harsh environments

The Physiological and Agronomic Responses to Nitrogen Dosage in Different Sugarcane Varieties

Identification and expression analyses of the alanine aminotransferase (AlaAT) gene family in poplar seedlings

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