Improved osteogenesis and angiogenesis of a novel copper ions doped calcium phosphate cement

Lin, Zefeng; Cao, Yannan; Zou, Jianming; Zhu, Fangyong; Gao, Yufeng; Zheng, Xiaofei; Wang, Huajun; Tao, Zhang; Wu, Tingting

doi:10.1016/j.msec.2020.111032

Cited by 60 publications

(36 citation statements)

References 50 publications

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“…It is well known that induced pluripotent stem cells (iPSCs) [48], and ASCs are capable of endothelial cell differentiation, although induction by endothelial cell differentiation medium EGM-2MV showed differences in species, with rat ASCs differentiating more easily towards endothelial cells than human ASCs [49]. During bone healing, angiogenesis is a crucial factor, and many bone tissue engineering approaches; therefore, not only focus on the osteogenic potential, but also on the angiogenic potential [50]. For example, this osteo/angiogenic potential was addressed by a combination of poly lactic-co-glycolic acid incorporated with micro-nano bioactive glass and mouse bone mesenchymal stem cells [51] or β-tricalcium phosphate seeded with canine bone marrow mesenchymal stem cells [52].…”

Section: Discussionmentioning

confidence: 99%

Suspension of Amorphous Calcium Phosphate Nanoparticles Impact Commitment of Human Adipose-Derived Stem Cells In Vitro

Wolint

Näf

Schibler

et al. 2021

Biology

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Amorphous calcium phosphate (aCaP) nanoparticles may trigger the osteogenic commitment of adipose-derived stem cells (ASCs) in vitro. The ASCs of three human donors are investigated using basal culture medium DMEM to either 5 or 50 µg/mL aCaP nanoparticles suspension (control: no nanoparticles). After 7 or 14 days, stem cell marker genes, as well as endothelial, osteogenic, chondrogenic, and adipogenic genes, are analyzed by qPCR. Free calcium and phosphate ion concentrations are assessed in the cell culture supernatant. After one week and 5 µg/mL aCaP, downregulation of osteogenic markers ALP and Runx2 is found, and averaged across the three donors. Our results show that after two weeks, ALP is further downregulated, but Runx2 is upregulated. Endothelial cell marker genes, such as CD31 and CD34, are upregulated with 50 µg/mL aCaP and a 2-week exposure. Inter-donor variability is high: Two out of three donors show a significant upregulation of ALP and Runx2 at day 14 with 50 µg/mL aCaP compared to 5 µg/mL aCaP. Notably, all changes in stem cell commitment are obtained in the absence of an osteogenic medium. While the chemical composition of the culture medium and the saturation status towards calcium phosphate phases remain approximately the same for all conditions, gene expression of ASCs changes considerably. Hence, aCaP nanoparticles show the potential to trigger osteogenic and endothelial commitment in ASCs.

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Section: Discussionmentioning

confidence: 99%

Suspension of Amorphous Calcium Phosphate Nanoparticles Impact Commitment of Human Adipose-Derived Stem Cells In Vitro

Wolint

Näf

Schibler

et al. 2021

Biology

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“…On the other hand, blue calcium phosphate cement with copper ions in another study showed a synergized dual antibacterial effect and cytocompatibility [ 89 ]. Many studies have shown that calcium phosphate cement with copper phosphate nanoparticles could promote vascularized new bone formation around cancerous bone defects [ 90 , 91 ]. On the other hand, a copper-modified zinc oxide phosphate cement showed low surface allocations of copper but no improvement in its antimicrobial properties [ 92 ].…”

Section: Copper Nanoparticles In Dental Materialsmentioning

confidence: 99%

Application of Copper Nanoparticles in Dentistry

Nizami

Yin

et al. 2022

Nanomaterials

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Nanoparticles based on metal and metallic oxides have become a novel trend for dental applications. Metal nanoparticles are commonly used in dentistry for their exclusive shape-dependent properties, including their variable nano-sizes and forms, unique distribution, and large surface-area-to-volume ratio. These properties enhance the bio-physio-chemical functionalization, antimicrobial activity, and biocompatibility of the nanoparticles. Copper is an earth-abundant inexpensive metal, and its nanoparticle synthesis is cost effective. Copper nanoparticles readily intermix and bind with other metals, ceramics, and polymers, and they exhibit physiochemical stability in the compounds. Hence, copper nanoparticles are among the commonly used metal nanoparticles in dentistry. Copper nanoparticles have been used to enhance the physical and chemical properties of various dental materials, such as dental amalgam, restorative cements, adhesives, resins, endodontic-irrigation solutions, obturation materials, dental implants, and orthodontic archwires and brackets. The objective of this review is to provide an overview of copper nanoparticles and their applications in dentistry.

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“…Copper is well known to support and accelerate angiogenesis. 23 Tissue engineering approaches including a copper component have been reported for bone regeneration, 40,41 for chronic wound healing 42 and for general stimulation of vessel growth within tissue-engineered constructs. 43 Accordingly, we developed a nanocomposite with CuO nanoparticles in electrospun, biodegradable, and porous PLGA/aCaP/CuO meshes.…”

Section: Grafts For Chest Wall Repairmentioning

confidence: 99%

Hybrid nanocomposite as a chest wall graft with improved vascularization by copper oxide nanoparticles

et al. 2022

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Chest wall repair can be necessary after tumor resection or chest injury. In order to cover or replace chest wall defects, autologous tissue or different synthetic materials are commonly used, among them the semi-rigid gold standard Gore-Tex® and prolene meshes. Synthetic tissues include composite materials with an organic and an inorganic component. On the basis of previously reported hybrid nanocomposite poly-lactic-co-glycolic acid amorphous calcium phosphate nanocomposite (PLGA/aCaP), a CuO component was incorporated to yield (60%/35%/5%). This graft was tested in vitro by seeding with murine adipose-derived stem cells (ASCs) for cell attachment and migration. The graft was compared to PLGA/CaCO3 and PLGA/hydroxyapatite, each providing the inorganic phase as nanoparticles. Further characterization of the graft was performed using scanning electron microscopy. Furthermore, PLGA/aCaP/CuO was implanted as a chest wall graft in mice. After 4 weeks, total cell density, graft integration, extracellular matrix components such as fibronectin and collagen I, the cellular inflammatory response (macrophages, F4/80 and lymphocytes, CD3) as well as vascularization (CD31) were quantitatively assessed. The nanocomposite PLGA/aCaP/CuO showed a good cell attachment and cells migrated well into the pores of the electrospun meshes. Cell densities did not differ between PLGA/aCaP/CuO and PLGA/CaCO3 or PLGA/hydroxyapatite, respectively. When applied as a chest wall graft, adequate stability for suturing into the thoracic wall could be achieved. Four weeks post-implantation, there was an excellent tissue integration without relevant fibrotic changes and a predominating collagen I matrix deposition within the graft. Slightly increased inflammation, reflected by increased infiltration of macrophages could be observed. Vascularization of the graft was significantly enhanced when compared with PLGA/aCaP (no CuO). We conclude that the hybrid nanocomposite PLGA/aCaP/CuO is a viable option to be used as a chest wall graft. Surgical implantation of the material is feasible and provides stability and enough flexibility. Proper tissue integration and an excellent vascularization are characteristics of this biodegradable material.

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Improved osteogenesis and angiogenesis of a novel copper ions doped calcium phosphate cement

Cited by 60 publications

References 50 publications

Suspension of Amorphous Calcium Phosphate Nanoparticles Impact Commitment of Human Adipose-Derived Stem Cells In Vitro

Suspension of Amorphous Calcium Phosphate Nanoparticles Impact Commitment of Human Adipose-Derived Stem Cells In Vitro

Application of Copper Nanoparticles in Dentistry

Hybrid nanocomposite as a chest wall graft with improved vascularization by copper oxide nanoparticles

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