2000
DOI: 10.1097/00006534-200012000-00015
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Improved Perfusion after Subcritical Ischemia in Muscle Flaps Treated with Vascular Endothelial Growth Factor

Abstract: Vascular endothelial growth factor (VEGF), a potent endothelial mitogen, is secreted in ischemic tissue and plays a pivotal role in angiogenesis. We studied whether VEGF administered to a rat muscle flap at the time of ischemia induction would increase microcirculatory flow to the flap. The cremaster muscle flap was isolated on its neurovascular pedicle. Ischemia was induced by clamping the vascular pedicle, and 0.2 ml of either VEGF (0.1 microg) or vehicle (phosphate-buffered saline) was immediately infused i… Show more

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Cited by 45 publications
(23 citation statements)
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“…Following the concept of therapeutic angiogenesis, 37 several factors, including transforming growth factor, basic fibroblast growth factor (bFGF), and VEGF have demonstrated marked abilities to improve skin flap survival 17,18,24 . Experimental studies have demonstrated that administration of exogenous VEGF can induce flap angiogenesis, improve the survival of random extensions of axial‐pattern skin flaps, and accelerate flap prefabrication 11,24 . The half‐life of VEGF protein under normal conditions is approximately 30–45 minutes, but this biological activity increases to 6–8 hours under ischemic conditions 38,39 .…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Following the concept of therapeutic angiogenesis, 37 several factors, including transforming growth factor, basic fibroblast growth factor (bFGF), and VEGF have demonstrated marked abilities to improve skin flap survival 17,18,24 . Experimental studies have demonstrated that administration of exogenous VEGF can induce flap angiogenesis, improve the survival of random extensions of axial‐pattern skin flaps, and accelerate flap prefabrication 11,24 . The half‐life of VEGF protein under normal conditions is approximately 30–45 minutes, but this biological activity increases to 6–8 hours under ischemic conditions 38,39 .…”
Section: Discussionmentioning
confidence: 99%
“…22 The beneficial effects of local or systemic administration of VEGF either as a recombinant protein, a plasmid vector or an adenovirus have been studied. 19,[23][24][25] Various studies have shown that the direct administration of plasmids has several advantages, such as the unlimited size of recombinant constructs, the established stability of DNA under a wide variety of conditions, and the proven safety of pure naked DNA in vivo. 26 Unlike viruses, plasmid-based gene transfer vehicles do not require carrier proteins for delivery of DNA into the cell, thereby bypassing local inflammatory responses that may reduce the therapeutic benefit of viral vectors.…”
Section: Introductionmentioning
confidence: 99%
“…Advances in vascular biology suggest that agents like growth factors, which can stimulate blood vessel formation (neoangiogenesis or arteriogenesis), will improve the healing process. Such agents include transforming growth factor-␤ (TGF␤) [58,59], basic fibroblast growth factor (b-FGF) [60,61], platelet-derived growth factor (PDGF) [62], and vascular endothelial growth factor (VEGF) [62][63][64][65][66][67][68][69][70][71]. Advanced age, diabetes or hypercholesterolemia have been proven to reduce the level of endogenous VEGF levels, coinciding with a prolonged or insufficient healing process.…”
Section: Wound Healing and Skin Repairmentioning
confidence: 99%
“…11,12 Improved wound healing with the application of exogenous VEGF has been shown in multiple myocutaneous flap models. [13][14][15][16][17] To date, VEGF gene therapy has not been employed within the gastrointestinal (GI) tract to promote anastomotic healing.…”
Section: Introductionmentioning
confidence: 99%