Improved production of biocatalysts by Yarrowia lipolytica using natural sources of the biopolyesters cutin and suberin, and their application in hydrolysis of poly (ethylene terephthalate) (PET)

Sales, Julio Cesar Soares; Castro, Aline Machado de; Ribeiro, Bernardo Dias; Coelho, Maria Alice Zarur

doi:10.1007/s00449-021-02603-w

Cited by 7 publications

(8 citation statements)

References 66 publications

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“…In these hydrolysis assays using cell-free enzymatic extracts, two different substrates were evaluated, PC-PET and BHET, a key intermediate in PET hydrolysis. The biocatalysts used in this step were produced by Y. lipolytica through solid-state fermentation (SSF) in media containing soybean bran and post-consumer PET (PC-PET) (20 wt%), as previously defined by Sales et al [ 18 ]. Another enzyme used in the process was Lipase B from Candida antarctica (CALB), which is already reported in the literature as an enzyme of interest in the hydrolysis of BHET [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

“…SSF was conducted in tray-type reactors containing 10 g of substrate. As a substrate, a mixture of soybean bran and PC-PET (20 wt%) was used, as previously defined by Sales et al [ 18 ]. Moisture was adjusted using an emulsion of water and soybean oil (1.5 wt%) once optimized by Souza et al [ 42 ].…”

Section: Methodsmentioning

confidence: 99%

“…In 2016, Yoshida et al [ 16 ] reported the isolation of a bacterium from plastic waste capable of producing an enzyme with a high affinity for PET, defined as PETase (EC 3.1.1.101). Other microorganisms have been reported to be able to act during PET depolymerization, including Yarrowia lipolytica [ 17 , 18 ]. Y. lipolytica is a nonconventional yeast widely studied for its intense secretory activity and ability to produce several biomolecules of industrial interest (e.g., organic acids, lipases, esterases and proteases) [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

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Post-Consumer Poly(ethylene terephthalate) (PET) Depolymerization by Yarrowia lipolytica: A Comparison between Hydrolysis Using Cell-Free Enzymatic Extracts and Microbial Submerged Cultivation

et al. 2022

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Several microorganisms have been reported as capable of acting on poly(ethylene terephthalate) (PET) to some extent, such as Yarrowia lipolytica, which is a yeast known to produce various hydrolases of industrial interest. The present work aims to evaluate PET depolymerization by Y. lipolytica using two different strategies. In the first one, biocatalysts were produced during solid-state fermentation (SSF-YL), extracted and subsequently used for the hydrolysis of PET and bis(2-hydroxyethyl terephthalate) (BHET), a key intermediate in PET hydrolysis. Biocatalysts were able to act on BHET, yielding terephthalic acid (TPA) (131.31 µmol L−1), and on PET, leading to a TPA concentration of 42.80 µmol L−1 after 168 h. In the second strategy, PET depolymerization was evaluated during submerged cultivations of Y. lipolytica using four different culture media, and the use of YT medium ((w/v) yeast extract 1%, tryptone 2%) yielded the highest TPA concentration after 96 h (65.40 µmol L−1). A final TPA concentration of 94.3 µmol L−1 was obtained on a scale-up in benchtop bioreactors using YT medium. The conversion obtained in bioreactors was 121% higher than in systems with SSF-YL. The results of the present work suggest a relevant role of Y. lipolytica cells in the depolymerization process.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Post-Consumer Poly(ethylene terephthalate) (PET) Depolymerization by Yarrowia lipolytica: A Comparison between Hydrolysis Using Cell-Free Enzymatic Extracts and Microbial Submerged Cultivation

et al. 2022

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“…Over the last 30 years, Y. lipolytica yeast has been utilized for various applications, including the production of biodiesel [42], oils and fats processing [43], biosynthesis of aromas, antimicrobial or antioxidant esters [32,[44][45][46][47][48][49], as well as polymer degradation [50,51].…”

Section: Substratementioning

confidence: 99%

“…Biodiesel is a type of fuel that is derived from renewable sources such as vegetable oils, animal fats, or recycled cooking oils. Typically, it is a mixture of methyl or ethyl es- Over the last 30 years, Y. lipolytica yeast has been utilized for various applications, including the production of biodiesel [42], oils and fats processing [43], biosynthesis of aromas, antimicrobial or antioxidant esters [32,[44][45][46][47][48][49], as well as polymer degradation [50,51].…”

Section: Substratementioning

confidence: 99%

Yarrowia lipolytica Yeast: A Treasure Trove of Enzymes for Biocatalytic Applications—A Review

Zieniuk,

Jasińska,

Wierzchowska

et al. 2024

Fermentation

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Yarrowia lipolytica is a robust yeast species that has gained significant attention as a biofactory for various biotechnological applications and undoubtedly can be referred to as a hidden treasure trove due to boasting a diverse array of enzymes with wide-ranging applications in multiple industries, including biofuel production, food processing, biotechnology, and pharmaceuticals. As the biotechnology field continues to expand, Y. lipolytica is poised to play a pivotal role in developing eco-friendly and economically viable bioprocesses. Its versatility and potential for large-scale production make it a promising candidate for sustainably addressing various societal and industrial needs. The current review article aimed to highlight the diverse enzymatic capabilities of Y. lipolytica and provide a detailed analysis of its relevance in biocatalysis, including the use of whole-cell catalysts and isolated enzymes. The review focused on wild-type yeast strains and their species-dependant properties and selected relevant examples of Y. lipolytica used as a host organism for overexpressing some enzymes. Furthermore, the application of Y. lipolytica’s potential in enantiomers resolution, lipids processing, and biodiesel synthesis, as well as the synthesis of polymers or esterification of different substrates for upgrading biologically active compounds, was discussed.

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Fungal and enzymatic bio-depolymerization of waste post-consumer poly(ethylene terephthalate) (PET) bottles using Penicillium species

Moysés

Teixeira²,

Waldow

et al. 2021

3 Biotech

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Improved production of biocatalysts by Yarrowia lipolytica using natural sources of the biopolyesters cutin and suberin, and their application in hydrolysis of poly (ethylene terephthalate) (PET)

Cited by 7 publications

References 66 publications

Post-Consumer Poly(ethylene terephthalate) (PET) Depolymerization by Yarrowia lipolytica: A Comparison between Hydrolysis Using Cell-Free Enzymatic Extracts and Microbial Submerged Cultivation

Post-Consumer Poly(ethylene terephthalate) (PET) Depolymerization by Yarrowia lipolytica: A Comparison between Hydrolysis Using Cell-Free Enzymatic Extracts and Microbial Submerged Cultivation

Yarrowia lipolytica Yeast: A Treasure Trove of Enzymes for Biocatalytic Applications—A Review

Fungal and enzymatic bio-depolymerization of waste post-consumer poly(ethylene terephthalate) (PET) bottles using Penicillium species

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