Improved production of chlorogenic acid from cell suspension cultures of &lt;i&gt;Lonicera macranthoids&lt;/i&gt;

Qun, LI; Ming, Tang; Tan, Yunya; Ma, Deying; Wang, Yanan; Zhang, Hong

doi:10.4314/tjpr.v15i5.4

Cited by 14 publications

(8 citation statements)

References 9 publications

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“…A previous study by Li et al . 18 found that inoculums size at 75 g/L produced the highest biomass with 17.16 g/L and the highest chlorogenic acid content of Lonicera macranthoids cell suspension cultures. A study conducted by Yang et al .…”

Section: Resultsmentioning

confidence: 91%

Factors Affecting Cell Biomass and Flavonoid Production of Ficus deltoidea var. kunstleri in Cell Suspension Culture System

Haida

Ahmad

Ariff

et al. 2019

Sci Rep

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A study was conducted to establish in vitro culture conditions for maximum production of biomass and flavonoid content for Ficus deltoidea var. kunstleri , locally named as Mas Cotek, known to have a wide variety of potential beneficial attributes for human health. Size of initial inoculum, cell aggregate and initial pH value have been suggested to influent content of biomass and flavonoid for cell suspension culture in several plant species. In the present study, leaf explants were cultured by cell suspension culture procedures in MSB5 basal medium supplemented with predetermined supplements of 30 g/L sucrose, 2.75 g/L gelrite, 2 mg/L picloram and 1 mg/L kinetin with continuous agitation of 120 rpm in a standard laboratory environment. Establishment of cell suspension culture was accomplished by culturing resulting callus in different initial fresh weight of cells (0.10, 0.25, 0.50, 1.0, and 2.0 g/25 mL of media) using similar basal medium. The results showed that the highest production of biomass (0.65 g/25 mL of media) was recorded from an initial inoculum size of 2.0 g/25 mL media, whereas the highest flavonoid (3.3 mg RE/g DW) was found in 0.5 g/25 mL of media. Cell suspension fractions classified according to their sizes (500–750 µm, 250–500 µm, and <250 µm). Large cell aggregate size (500–750 µm) cultured at pH 5.75 produced the highest cell biomass (0.28 g/25 mL media) and flavonoid content (3.3 mg RE/g DW). The study had established the optimum conditions for the production of total antioxidant and flavonoid content using DPPH and FRAP assays in cell suspension culture of F . deltoidea var. kunstleri .

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Section: Resultsmentioning

confidence: 91%

Factors Affecting Cell Biomass and Flavonoid Production of Ficus deltoidea var. kunstleri in Cell Suspension Culture System

Haida

Ahmad

Ariff

et al. 2019

Sci Rep

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“…In the previous report enhanced production of biomass and alkaloid accumulation was optimized for the composition and strength of nutrient media, carbon source and plant growth regulators [31][32][33]. The normal or single strength B5 medium supplanted with 3% sucrose (w/v) was found as the best choice for the optimal biomass accumulation and production of secondary metabolite in cell suspension culture of L. macranthoids [39] and C. roseus [31][32][33]. Li et al [39] observed similar trends in biomass and chlorogenic acid production using 6-BA (2.0 mg/L) and NAA (0.5 mg/L) in B5 suspension culture of Lonicera macranthoids.…”

Section: Discussionmentioning

confidence: 99%

“…The normal or single strength B5 medium supplanted with 3% sucrose (w/v) was found as the best choice for the optimal biomass accumulation and production of secondary metabolite in cell suspension culture of L. macranthoids [39] and C. roseus [31][32][33]. Li et al [39] observed similar trends in biomass and chlorogenic acid production using 6-BA (2.0 mg/L) and NAA (0.5 mg/L) in B5 suspension culture of Lonicera macranthoids. In the present study production of biomass (fresh wt.…”

Section: Discussionmentioning

confidence: 99%

“…The growth and ginsenoside production in hairy root culture of ginseng was maximum when the initial pH of the medium adjusted between 6.0 to 6.5 prior to autoclaving and significantly reduced at initial pH 4.0 or lower or above 7.0 [47]. Li et al [39] studied the effect of pH on the production biomass and chlorogenic acid in cell suspension cultures of Lonicera macranthoids. The highest biomass of 6.52 g/L and 6.76 g/L was achieved at 5.5 and 6.0 media pH respectively, showing maximum chlorogenic acid content.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Nitrogen, Phosphorus and Medium pH to Enhance Alkaloid Production from Catharanthus roseus Cell Suspension Culture

Mishra

Srivastava

Akhtar

2019

International Journal of Secondary Metabolite

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Several elevated levels of nitrogen and phosphate at varying pH of the medium which impart a major influence on callus and biomass development and subsequent production of alkaloids was investigated using suspension culture system of Catharanthus roseus in the present study. The B5 medium was buffered at pH 4.51, 5.82 and 7.32 by addition of different levels of (A) diammonium hydrogen phosphate (NH4)2HPO4 and (B) ammonium dihydrogen orthophosphate (NH4H2PO4) representing the enhanced and varied supply of total nitrogen (NH4 + +NO3 ˉ) and phosphate compared to MS medium (as control) for cell biomass production and alkaloid yield. The pH of the medium have shown significant effects with maximum biomass fresh wt., dry wt. and total alkaloid yield at 5.82 medium pH with elevated phosphate levels and total nitrogen concentration of 3710.10 mg/L compared to control MS medium with 2850 mg/L total nitrogen. At 3667.33 and 3752.48 mg/L of total nitrogen with enhanced phosphate supply showed reduced biomass fresh wt., dry wt. and total alkaloid yield at lower (4.51) and higher (7.32) medium pH respectively. Inclusion of 200 mg/L of tryptophan or phenylalanine as reduced nitrogen source in B5 medium buffered at 5.82 ± 0.2 pH showed enhanced biomass and alkaloid production. Hence, addition of nitrogen, phosphate, tryptophan, phenylalanine as nutrient in suspension culture stimulate their uptake to enhance cell biomass and total alkaloids production but as a function of pH of the medium.

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“…The production of chlorogenic acid in the cell suspension cultures was already studied for some medicinal plants i.e. : Eryngium planum [24], Eucommia ulmoides [25], Lonicera macranthoids [26], Coffea arabica [27].…”

Section: Resultsmentioning

confidence: 99%

Pentacyclic triterpenoids and polyphenols accumulation in cell suspension culture of Chaenomeles japonica (Thunb.) Lindl. ex Spach

et al. 2019

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Summary Introduction: Callus and cell suspension cultures are widely applied in investigation of production of high-value secondary metabolites, which may be used as cosmeceuticals, nutraceuticals and pharmaceuticals. Plant cell cultures are promising alternative to intact plant sources for the production of plant-derived drugs of industrial importance. Objective: The aim of the study was to (i) initiate the cell suspension culture of Chaenomeles japonica from homogenous and uniform callus, (ii) stabilize the selected line and (iii) verify its ability to produce the desired groups of secondary metabolites – pentacyclic triterpenoids and polyphenols. Methods: To establish a cell suspension culture, stabilized and homogeneous callus was selected. Cell cultures were systematically passaged every 2 weeks to fresh liquid medium with the same composition. Biomass from cultures at the growth phase and stationary phase was designated for phytochemical research. UHPLC-DAD-MS analyzes were performed. At the same time, their macroscopic and microscopic observations were carried out. Results: Cells of suspension culture line A2 were characterized by the intense divisions. Cell culture extracts (both from the growth phase and stationary phase) contained pentacyclic triterpenoids. In addition, phe-nolic compounds (chlorogenic acid and proanthocyanidins type B) and in a small amount also epicatechin are present in the extract of the cells harvested from the growth phase. In the present studies, three pentacyclic triterpenoids were detected and quantified in the extracts of cell suspensions and callus line A2. Ursolic and oleanolic acids were the main triterpenoids in the studied extracts. The cell suspension culture from the growth phase exhibited the highest content of ursolic, oleanolic, and betulinic acid (separately and together). Conclusion: The cell suspension culture of Chaenomeles japonica is a promising source of pentacyclic triterpenoids.

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Improved production of chlorogenic acid from cell suspension cultures of <i>Lonicera macranthoids</i>

Cited by 14 publications

References 9 publications

Factors Affecting Cell Biomass and Flavonoid Production of Ficus deltoidea var. kunstleri in Cell Suspension Culture System

Factors Affecting Cell Biomass and Flavonoid Production of Ficus deltoidea var. kunstleri in Cell Suspension Culture System

Effect of Nitrogen, Phosphorus and Medium pH to Enhance Alkaloid Production from Catharanthus roseus Cell Suspension Culture

Pentacyclic triterpenoids and polyphenols accumulation in cell suspension culture of Chaenomeles japonica (Thunb.) Lindl. ex Spach

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