Improved production of curdlan with concentrated cells ofAgrobacterium sp.

Jung, Dae-Young; Cho, Young-Su; Chung, Chong-Pyoung; Jung, Dai‐Il; Kim, Kwang; Lee, Jin Woo

doi:10.1007/bf02931955

Cited by 12 publications

(5 citation statements)

References 21 publications

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“…Curdlan production was not seen during the cell growth phase and nutrient limitation was required for initiation of curdlan biosynthesis (Lee at al. 1997& Jung et al 2001). Nitrogen source in the medium is considered as a critical factor in the change of intracellular metabolism, because isoprenoid lipids that play a vital role in carrying cellular oligosaccharides would be more available for curdlan synthesis, instead of cellular lipopolysaccharide synthesis under nitrogen limiting conditions (Lee et al 1997).…”

Section: Introductionmentioning

confidence: 96%

Production and downstream processing of (1→3)-β-D-glucan from mutant strain of Agrobacterium sp. ATCC 31750

2012

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We isolated a mutant that produced higher levels of curdlan than the wild strain Agrobacterium sp. ATCC 31750 by chemical mutagenesis using N-methyl-N-nitro-nitrosoguanidine. The mutant strain produced 66 g/L of curdlan in 120 h with a yield of (0.88) while, the wild strain produced 41 g/L in 120 h with a yield of (0.62) in a stirred bioreactor. The mutant could not produce curdlan when the pH was shifted from 7.0 to 5.5 after nitrogen depletion as followed for wild strain. In contrast, pH optimum for cell growth and curdlan production for mutant was found to be 7.0. We optimized the downstream processing of curdlan by varying different volumes of NaOH and HCl for extraction and precipitation of curdlan. The molecular weight of the purified curdlan from the wild and mutant strain was 6.6 × 105 Da and 5.8 × 105 Da respectively. The monosaccharide analyses confirm that curdlan from both wild and mutant strain contains only glucose units. From the NMR and FTIR data, it has been confirmed that curdlan was exclusively composed of β (1 → 3)-D-glucan residues.

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Section: Introductionmentioning

confidence: 96%

Production and downstream processing of (1→3)-β-D-glucan from mutant strain of Agrobacterium sp. ATCC 31750

2012

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“…sium phosphate as a pH stabililizer (•, pH; █, dissolved oxygen; ▲, DCW; ○, gellan and □, reducing sugar). Production of gellan by S. paucibilis NK2000 under pH control was higher than that without pH control, as in production of other microbial exopolysaccharides [14,16,31]. But the cost of equipment for pH control in a batch culture, as well as in a continuous culture, is expensive and there is a possibility of contamination in the pH control process [22].…”

Section: Resultsmentioning

confidence: 99%

“…The conversion carbon source to curdlan by Agrobacterium sp. is about 50% if the pH of the culture medium is maintained at around 6.0 [16]. The pH of the culture medium influences not only production of pullulan by Aureobasidum pullulan, but also the morphology of this strain [19].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Potassium Phosphate as a pH Stabilizer on the Production of Gellan by Sphingomonas paucibilis NK-2000

Lee¹,

Jo²,

Jin³

et al. 2009

Journal of Life Science

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“…The production of PS-7 by B. indica favors a high ratio of carbon to nitrogen as substrate, similar to productions of other microbial polysaccharides [27]. Productions of curdlan by Agrobacterium sp., pullulan by A. pullulans, and xanthan by X. campestris were stimulated by nitrogen limitation [28][29][30]. Pullulan was only produced under conditions of N-limitation in a batch culture [29].…”

Section: Effect Of Composition Of Substituted Solution On Production mentioning

confidence: 96%

Enhanced production of heteropolysaccharide-7 by Beijerinckia indica HS-2001 in repeated batch culture with optimized substitution of culture medium

Jin

Jung

Son

et al. 2011

Biotechnol Bioproc E

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In this study, a process of removing a half volume of culture broth and replacing it with an equal volume of substituted solution was developed to enhance the production of heteropolysaccharide-7 (PS-7) by Beijerinckia indica HS-2001. The optimal substitution time and volume of the substituted solution were found to be 48 h after cultivation and 50% of the initial volume of the culture broth. The optimal composition of the substituted solution was determined to be 20.0 g/L glucose, 10.0 g/L soybean pomace, 0.1 g/L MgSO 4 ·7H 2 O, 0.9 g/L NH 4 NO 3 , and 5.0 g/L potassium phosphate, which was the same composition as the medium developed in a previous study for the production of PS-7 by B. indica HS-2001. The total amount and productivity of PS-7 by B. indica HS-2001 with a substitution under optimized conditions in a 7 L bioreactor for 96 h were 49.28 g and 0.51 g/h, respectively, which were 1.76 and 1.31-foldgreater values than those without a substitution for 72 h.

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Improved production of curdlan with concentrated cells ofAgrobacterium sp.

Cited by 12 publications

References 21 publications

Production and downstream processing of (1→3)-β-D-glucan from mutant strain of Agrobacterium sp. ATCC 31750

Production and downstream processing of (1→3)-β-D-glucan from mutant strain of Agrobacterium sp. ATCC 31750

The Effect of Potassium Phosphate as a pH Stabilizer on the Production of Gellan by Sphingomonas paucibilis NK-2000

Enhanced production of heteropolysaccharide-7 by Beijerinckia indica HS-2001 in repeated batch culture with optimized substitution of culture medium

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