Improved Raoultella planticola Strains for the Production of 2,3-Butanediol from Glycerol

Abstract

Raw glycerol is an industrial byproduct from biodiesel production and is one of the most promising substrates for 2,3-butanediol (2,3-BD) production; however, 2,3-BD is not yet produced by fermentation from glycerol on a commercial scale due to poor process economics. Class 1 microorganism collections were screened and Raoultella planticola strain CECT 843 proved to be the best 2,3-BD producer, achieving (23.3 ± 1.4) g 2,3-BD per L and a yield of 36% (g 2,3-BD per g glycerol). To further increase product concentration and yield, R. planticola CEC T843 was subjected to random mutagenesis using ultra-violet (UV) light and ethyl methane sulfonate (EMS). Two mutant strains were found to produce at least 30% more 2,3-BD than the wild type: R. planticola IA1 [(30.8 ± 3.9) g 2,3-BD per L and 49% yield] and R. planticola IIIA3 [(30.5 ± 0.4) g 2,3-BD per L and 49% yield].

Enhanced 2,3-Butanediol production by mutant Enterobacter ludwigii using Brewers’ spent grain hydrolysate: Process optimization for a pragmatic biorefinery loom

“…strain XRM21 to random mutagenesis using EMS which resulted in a highly productive mutant strain accumulating 119.4 g/L in 65 h against 91.2 g/L BDO generated by wild type strain [50]. In another report by Bustamante et al, (2019), two mutant strains were identified after treatment with EMS accumulating 30% more BDO (~31 g/L) than the wild type, with yield approximating the theoretical maximum [51].…”

Bioglycerol (C3) upgrading to 2,3‐butanediol (C4) by cell‐free extracts of Enterobacter aerogenes NCIM 2695

“…Another commercial product (1,3‐butadiene) is used extensively in rubber production and can be obtained by the dehydration of bio‐derived 2,3‐BDO 8,9 . Several other applications of 1,3‐butadiene include as a solvent for resins and lacquers, antifreeze agent, octane booster analytical reagent, as well as in cosmetics, perfumery, 10 printing inks, 11 as a plasticizer, 12 and in the synthesis of various pharmaceutical derivatives 13‐16 . Thus, the bio‐based production of 2,3‐BDO can eventually become a sustainable route for all these industrial commodities 17,18 …”

“…Bioglycerol, an inevitable by‐product of biodiesel production, could be the most suitable feedstock for 2,3‐BDO synthesis 14,19,20 . The commercialization of several biodiesel plants worldwide has resulted in the availability of huge quantities of bioglycerol at the very low price of 3.5–10 cents lb −1 , which has created the serious problem of its proper disposal/utilization 21 .…”

Production of Bio-Based Chemicals and Polymers from Renewable Feedstocks: Bioprocesses Development

“…LIST OF PUBLICATIONS Bustamante, D., Segarra, S., Montesinos, A., Tortajada, M., Ramón, D., Rojas, A. (2019) Improved Raoultella planticola strains for the production of 2,3-butanediol from glycerol.…”