Enhanced 2,3-Butanediol production by mutant Enterobacter ludwigii using Brewers’ spent grain hydrolysate: Process optimization for a pragmatic biorefinery loom

Amraoui, Yassin; Prabhu, Ashish A.; Narisetty, Vivek; Coulon, Frédéric; Chandel, Anuj K.; Willoughby, Nicholas; Jacob, Samuel; Koutinas, Apostolis; Kumar, Vinod

doi:10.1016/j.cej.2021.130851

Cited by 42 publications

(48 citation statements)

References 62 publications

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“…The economy of the production of chemicals like 2,3-BD mostly depends on the used renewable raw material which is also a carbon source. The use of cheap residual materials such as BSG can greatly reduce production costs and thus make the production process economically and environmentally sustainable (Amraoui et al, 2021). Due to its high content of polysaccharides, BSG have a great potential for application in the biotechnological industry (Ferraz et al, 2013;Mussatto, 2014;Paz et al, 2019;Jackowski et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Production of Different Biochemicals by Paenibacillus polymyxa DSM 742 From Pretreated Brewers’ Spent Grains

et al. 2022

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Brewers’ spent grains (BSG) are a by-product of the brewing industry that is mainly used as feedstock; otherwise, it has to be disposed according to regulations. Due to the high content of glucose and xylose, after pretreatment and hydrolysis, it can be used as a main carbohydrate source for cultivation of microorganisms for production of biofuels or biochemicals like 2,3-butanediol or lactate. 2,3-Butanediol has applications in the pharmaceutical or chemical industry as a precursor for varnishes and paints or in the food industry as an aroma compound. So far, Klebsiella pneumoniae, Serratia marcescens, Clostridium sp., and Enterobacter aerogenes are being used and investigated in different bioprocesses aimed at the production of 2,3-butanediol. The main drawback is bacterial pathogenicity which complicates all production steps in laboratory, pilot, and industrial scales. In our study, a gram-positive GRAS bacterium Paenibacillus polymyxa DSM 742 was used for the production of 2,3-butanediol. Since this strain is very poorly described in literature, bacterium cultivation was performed in media with different glucose and/or xylose concentration ranges. The highest 2,3-butanediol concentration of 18.61 g l–1 was achieved in medium with 70 g l–1 of glucose during 40 h of fermentation. In contrast, during bacterium cultivation in xylose containing medium there was no significant 2,3-butanediol production. In the next stage, BSG hydrolysates were used for bacterial cultivation. P. polymyxa DSM 742 cultivated in the liquid phase of pretreated BSG produced very low 2,3-butanediol and ethanol concentrations. Therefore, this BSG hydrolysate has to be detoxified in order to remove bacterial growth inhibitors. After detoxification, bacterium cultivation resulted in 30 g l–1 of lactate, while production of 2,3-butanediol was negligible. The solid phase of pretreated BSG was also used for bacterium cultivation after its hydrolysis by commercial enzymes. In these cultivations, P. polymyxa DSM 742 produced 9.8 g l–1 of 2,3-butanediol and 3.93 g l–1 of ethanol. On the basis of the obtained results, it can be concluded that different experimental setups give the possibility of directing the metabolism of P. polymyxa DSM 742 toward the production of either 2,3-butanediol and ethanol or lactate.

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Section: Discussionmentioning

confidence: 99%

Production of Different Biochemicals by Paenibacillus polymyxa DSM 742 From Pretreated Brewers’ Spent Grains

et al. 2022

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“…ludwigii is Gram-negative bacterium with a strong capability to manufacture BDO from a variety of carbon sources including glucose, fructose, xylose, arabinose, mannose, galactose, sucrose, glycerol etc. In our previous work, we have reported high fermentation efficiency of the bacterium to generate BDO using pure as well as crude glucose and xylose from brewer's spent grains and sugarcane bagasse, respectively [13,14]. In the current work, the bacterium was cultured on different concentrations of pure arabinose (10,20,30,40, 50 and 60 g/L).…”

Section: Shake Flask Cultivation Of E Ludwigii On Pure Arabinosementioning

confidence: 94%

“…An average molecular weight of 24.6, which corresponds to an average cell with a molecular formula of CH 1.8 O 0.5 N 0.2 was used for carbon analysis [18]. The sample processing for the High-Pressure Liquid Chromatography (HPLC) analysis were discussed elsewhere [13,14]. The samples were loaded into a HPLC system equipped with a Rezex ROA-Organic Acid H þ (Phenomenex, USA) column for elution connected with Refractive Index Detector (RID) for sugars, diols, alcohols, and a Diode Array Detector (DAD) for organic acids.…”

Section: Methodsmentioning

confidence: 99%

“…A mutant Enterobacter ludwigii strain developed in our previous study [13], which has been evaluated as a potent BDO producer was used in the current study. The strain was maintained on Tryptic Soy (TS) agar plates with the following composition (g/L): 17 pancreatic digest of casein, 3 soybean meal, 5 NaCl, 2.5, KH 2 PO 4 , 2.5 glucose and the pH was adjusted to 7.3 prior to sterilisation.…”

Section: Microorganism Cultivation and Maintenancementioning

confidence: 99%

“…BDO fermentations by E. ludwigii are accompanied by a drop in pH. The degree of pH reduction amplify with increase in substrate levels and the metabolism almost ceases when the pH falls below 5.0 [13,14]. The pH was manually adjusted by addition of alkali (5M NaOH) at regular intervals during the course of fermentation to prevent the pH falling below 6.0.…”

Section: Shake Flask Cultivation Of E Ludwigii On Pure Arabinosementioning

confidence: 99%

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