Improved resorcinol reagent for the determination of fructose, and of 3,6-anhydrogalactose in polysaccharides

Yaphe, W.; Arsenault, G. P.

doi:10.1016/0003-2697(65)90128-4

Cited by 394 publications

(149 citation statements)

References 11 publications

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“…8 Dextran sulfates of average molecular weight 8000, 10000, and 500000, as well as neocarrabiose General Procedures. Carbohydrate content was analyzed by phenol-sulfuric acid method 19 without previous hydrolysis of the polysaccharide, while 3,6-anhydrogalactose was estimated by the resorcinol method 20 . Sulfate was measured by the turbidimetric method of Dodgson and Price.…”

Section: Methodsmentioning

confidence: 99%

Autohydrolysis of a partially cyclized m/u-carrageenan and structural elucidation of the oligosaccharides by chemical analysis, NMR spectroscopy and UV-MALDI mass spectrometry

Ciancia¹,

Sato²,

Nonami³

et al. 2006

Arkivoc

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Dedicated to Prof. Rosa M. de Lederkremer on occasion of her 70 th anniversaryAbstract A partially cyclized mu/nu-carrageenan from Gigartina skottsbergii was submitted to autohydrolysis and the resulting mixture of oligosaccharides was fractionated using sequential dialysis through tubings of molecular weight cutoff 12000, 3500 and 1000. In this way four fractions were separated and characterized. Structural analysis of the major fraction, obtained from the solution inside the dialysis tubing of molecular weight cutoff 12000 (D120), was carried out. A significant difference was found by 13 C NMR spectroscopy and UV-MALDI-TOF mass spectrometry: in the 13 C NMR spectrum the reducing end-chain unit was the hydrated aldehyde of 3,6-anhydrogalactose while the UV-MALDI-TOF mass spectrum showed an unimodal distribution of even and odd peaks, suggesting fragmentation of glycosidic linkages.

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Section: Methodsmentioning

confidence: 99%

Autohydrolysis of a partially cyclized m/u-carrageenan and structural elucidation of the oligosaccharides by chemical analysis, NMR spectroscopy and UV-MALDI mass spectrometry

Ciancia¹,

Sato²,

Nonami³

et al. 2006

Arkivoc

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“…3,6-Anhydro-D-galactose was determined by method of Yaphe and Arsenault [49] using anhydro-β-D-galactose as a standard. Ash content was determined by incinerating the polysaccharide for 24 h in a muffle furnace at 550˚C and then weighed the residue.…”

Section: Chemical Component Analysismentioning

confidence: 99%

The Novel Pyruvated Glucogalactan Sulfate Isolated from the Red Seaweed, <i>Hypnea pannosa</i>

Tako¹,

Ohtoshi²,

Kinjyo³

et al. 2016

ABC

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“…3,6-anhydro sugar content was assayed by the resorcinol/hydrochloric acid method of Yaphe and Arsenault [13] taking methyl 3,6-anhydro-a-~-galactopyranoside as standard, and inorganic phosphate by the molybdate technique of Fiske and Subbarow ~4 1 .…”

Section: Miscellaneous Assaysmentioning

confidence: 99%

Glycosulphatase from Pseudomonas carrageenovora. Purification and Some Properties

McLEAN

Williamson

1979

Eur J Biochem

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A glycosulphatase present in the soluble fraction of disrupted Pseudomonas carrageenovora has been purified 500-fold by gel filtration on Sephacryl S-200 and ion-exchange chromatography on DEAE-Sepharose CL-6B. By dodecylsulphate/polyacrylamide gel electrophoresis the enzyme is practically homogeneous and has a molecular weight of 55 000. Conditions of optimal sodium chloride concentration and pH at 25 "C were 0.25-0.50 mol dm-3 and pH 7.0 respectively. The purified enzyme was inhibited by inorganic phosphate.Preparation is described of neocarrabiose 4-0-[35S]sulphate and neocarratetraose 4-0-[35S]-sulphate from labelled Chondrus crispus. The purified glycosulphatase is active against both these substrates although only one of the two sulphate esters in the tetrasaccharide is hydrolysed. Analysis of the reaction products was by gel filtration, electrophoresis and I3C nuclear magnetic resonance spectroscopy. The results are consistent with the products of desulphation being respectively neocarrabiose and neocarratetraose 4-0-monosulphate with the sulphate ester proximal to the reducing end [ 3,6-anhydro-a-~-galactopyranosyl-(Sulphatases have been detected ubiquitously in organisms [l -31 pertinent examples being bacteria [4,5] and algae [6,7]. In the degradation of chondroitin sulphate by Proteus vulgaris [4] only disaccharide oligomers are susceptible to the relevant sulphatases. This contrasts with the algal sulphatases [6,7], which release sulphate at the polymer level from galactose 6-0-sulphate residues with concomitant formation of the 3,6-anhydro derivative.In the initial description of a glycosulphatase from Pseudomonas carrageenovora by Weigl and Yaphe [5], the partially purified enzyme was tested with several sugar sulphates. We consider this enzyme to be potentially applicable in the study of carrageenan fragments, and present a satisfactory purification method.Preparation of neocarratetraose 4-0-[35S]sulphate and neocarratetraose 4-0-[35S]sulphate of high radiochemical purity allowed the development of a practical assay similar to that described for choline sulphatase [8]. These oligosaccharides were isolated after x-carrageenase digestion of [35S]carrageenan extracted from Chondrus crispus cultured in the presence of inorganic [35S]sulphate.

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Improved resorcinol reagent for the determination of fructose, and of 3,6-anhydrogalactose in polysaccharides

Cited by 394 publications

References 11 publications

Autohydrolysis of a partially cyclized m/u-carrageenan and structural elucidation of the oligosaccharides by chemical analysis, NMR spectroscopy and UV-MALDI mass spectrometry

Autohydrolysis of a partially cyclized m/u-carrageenan and structural elucidation of the oligosaccharides by chemical analysis, NMR spectroscopy and UV-MALDI mass spectrometry

The Novel Pyruvated Glucogalactan Sulfate Isolated from the Red Seaweed, <i>Hypnea pannosa</i>

Glycosulphatase from Pseudomonas carrageenovora. Purification and Some Properties

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Improved resorcinol reagent for the determination of fructose, and of 3,6-anhydrogalactose in polysaccharides

Cited by 394 publications

References 11 publications

Autohydrolysis of a partially cyclized m/u-carrageenan and structural elucidation of the oligosaccharides by chemical analysis, NMR spectroscopy and UV-MALDI mass spectrometry

Autohydrolysis of a partially cyclized m/u-carrageenan and structural elucidation of the oligosaccharides by chemical analysis, NMR spectroscopy and UV-MALDI mass spectrometry

The Novel Pyruvated Glucogalactan Sulfate Isolated from the Red Seaweed, &lt;i&gt;Hypnea pannosa&lt;/i&gt;

Glycosulphatase from Pseudomonas carrageenovora. Purification and Some Properties

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The Novel Pyruvated Glucogalactan Sulfate Isolated from the Red Seaweed, <i>Hypnea pannosa</i>