Improved RP-HPLC method to determine neferine in dog plasma and its application to pharmacokinetics

The In Vitro Inhibition of Human CYP1A2, CYP2D6 and CYP3A4 by Tetrahydropalmatine, Neferine and Berberine

“…The fact that studies in rats and dogs demonstrate a high bioavailability of Nef of about 65% (Zhao et al . ; Huang et al ., ) makes human pharmacokinetic studies warranted. On the other hand, the estimated IC 50 values for Nef on the CYP enzymes investigated are rather high, hardly indicating any systemic CYP interaction potential of Nef.…”

Neferine enhances insulin sensitivity in insulin resistant rats

“…Neferine is a bisbenzylisoquinline alkaloid extracted (isolated) from the green seed embryo of Nelumbo nucifera Gaertn (Zhao et al, 2007;Chen et al, 2007). The molecular formula of neferine was assigned by Tof-mass spectra (m/z 625 [M + 1] + ), and its structure ( Fig.…”

A Validated Method for the Simultaneous Quantitation of Bioactive Alkaloid Markers in the Leaf Ethanolic Extract of Cissampelos sympodialis Eichl.: a Phenological Variation Study

“…The determination of bisbenzylisoquinoline alkaloids in plants or extracts can be challenging due to the complexity of the sample matrix and the poor peak shape of the alkaloids under reversed‐phase chromatography conditions, often requiring the use of a combination of stationary phases such as C 18 and cyano (Tshibangu et al , ) or the use of ion‐pair chromatography (Sun et al , ). Another drawback of the available published methods is the lengthy and tedious liquid–liquid extraction of the alkaloids from the samples (Zhao et al , ). This can be simplified when mass spectrometry is used for quantitation in multiple reaction monitoring mode (usually with triple quadrupole instruments), when usually a single step to precipitate proteins from biological fluids such as plasma is required (Dong et al , ).…”