Improved spectral resolution of [13C,1H]-HSQC spectra of aromatic amino acid residues in proteins produced by cell-free synthesis from inexpensive 13C-labelled precursors

Raad, Damian Van; Huber, Thomas; Otting, Gottfried

doi:10.1007/s10858-023-00420-9

Cited by 4 publications

(2 citation statements)

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“…Enzymatic methods have facilitated access to 13 C-labelled metabolites, such as monosaccharides [163], as well as 13 C-labelled amino acids [164][165][166][167]. The use of very simple and inexpensive 13 C-labelled precursors is thereby attractive, such as 13 C-labelled pyruvate for the enzymatic synthesis of 13 C-labelled aromatic amino acids [168]. Direct utilization of 13 carbon dioxide is not only of significant interest for investigating the metabolism of photosynthetic organisms, but also for the biocatalytic synthesis of 13 C-labelled biochemicals, such as 13 C-labelled L-malate [169].…”

Section: Synthesis Of Isotope-labelled Metabolitesmentioning

confidence: 99%

Synthesis of Metabolites and Metabolite-like Compounds Using Biocatalytic Systems

Wohlgemuth

2023

Metabolites

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Methodologies for the synthesis and purification of metabolites, which have been developed following their discovery, analysis, and structural identification, have been involved in numerous life science milestones. The renewed focus on the small molecule domain of biological cells has also created an increasing awareness of the rising gap between the metabolites identified and the metabolites which have been prepared as pure compounds. The design and engineering of resource-efficient and straightforward synthetic methodologies for the production of the diverse and numerous metabolites and metabolite-like compounds have attracted much interest. The variety of metabolic pathways in biological cells provides a wonderful blueprint for designing simplified and resource-efficient synthetic routes to desired metabolites. Therefore, biocatalytic systems have become key enabling tools for the synthesis of an increasing number of metabolites, which can then be utilized as standards, enzyme substrates, inhibitors, or other products, or for the discovery of novel biological functions.

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Section: Synthesis Of Isotope-labelled Metabolitesmentioning

confidence: 99%

Synthesis of Metabolites and Metabolite-like Compounds Using Biocatalytic Systems

Wohlgemuth

2023

Metabolites

View full text Add to dashboard Cite

show abstract

“…Enzymatic methods have facilitated access to 13 C-labelled metabolites, such as monosaccharides [132] as well as 13 C-labelled amino acids [133][134][135][136]. The use of very simple and inexpensive 13 C-labelled precursors is thereby attractive, such as 13 C-labelled pyruvate for the enzymatic synthesis of 13 C-labelled aromatic amino acids [137]. Direct utilization of 13 carbon dioxide is not only of much interest for investigating the metabolism of photosynthetic organisms, but also for the biocatalytic synthesis of 13 C-labelled biochemicals, such as 13 C-labelled L-malate [138].…”

Section: Synthesis Of Isotope-labelled Metabolitesmentioning

confidence: 99%

Synthesis of Metabolites and Metabolite-Like Compounds Using Biocatalytic Systems

Wohlgemuth

2023

Preprint

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Methodologies for the synthesis and purification of metabolites, which have been developed following their discovery, analysis and structural identification, have been involved in numerous life science milestones. The renewed focus on the small molecule domain of biological cells has also created an increasing awareness of the rising gap between the metabolites identified and the metabolites which have been prepared as pure compounds. Due to the large number and molecular diversity of metabolites the design and engineering of resource-efficient and straightforward synthetic methodologies for their production have attracted much interest. The variety of metabolic pathways in biological cells provide a wonderful blueprint for designing simplified and resource-efficient synthetic routes to desired metabolites. Therefore, biocatalytic systems have become key enabling tools for the synthesis of an increasing number of metabolites, which can then be utilized as standards, enzyme substrates, inhibitors or products, or for the discovery of novel biological functions.

show abstract