Improved staining of F‐actin and co‐localization of mitochondria in plant cells

Olyslaegers, G.; Verbelen, Jean‐Pierre

doi:10.1046/j.1365-2818.1998.00398.x

Cited by 69 publications

(40 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…F-actin was stained using the previously described method (Olyslaegers and Verbelen, 1998;Yang et al, 2011;Zhang et al, 2011). Mature rice leaves were incubated in PEM buffer (100 mM PIPES, 10 mM EGTA, 0.3 M mannitol, and 5 mM MgSO 4 , pH 6.9) and 0.66 mM Alexa Fluor 488-phalloidin (Life Technologies).…”

Section: Fluorescence Microscopy Of Actin Filamentsmentioning

confidence: 99%

Rice TUTOU1 Encodes a Suppressor of cAMP Receptor-Like Protein That Is Important for Actin Organization and Panicle Development

et al. 2015

View full text Add to dashboard Cite

Panicle development, a key event in rice (Oryza sativa) reproduction and a critical determinant of grain yield, forms a branched structure containing multiple spikelets. Genetic and environmental factors can perturb panicle development, causing panicles to degenerate and producing characteristic whitish, small spikelets with severely reduced fertility and yield; however, little is known about the molecular basis of the formation of degenerating panicles in rice. Here, we report the identification and characterization of the rice panicle degenerative mutant tutou1 (tut1), which shows severe defects in panicle development. The tut1 also shows a pleiotropic phenotype, characterized by short roots, reduced plant height, and abnormal development of anthers and pollen grains. Molecular genetic studies revealed that TUT1 encodes a suppressor of cAMP receptor/Wiskott-Aldrich syndrome protein family verprolin-homologous (SCAR/WAVE)-like protein. We found that TUT1 contains conserved functional domains found in eukaryotic SCAR/WAVE proteins, and was able to activate Actin-related protein2/3 to promote actin nucleation and polymerization in vitro. Consistently, tut1 mutants show defects in the arrangement of actin filaments in trichome. These results indicate that TUT1 is a functional SCAR/WAVE protein and plays an important role in panicle development.

show abstract

Section: Fluorescence Microscopy Of Actin Filamentsmentioning

confidence: 99%

Rice TUTOU1 Encodes a Suppressor of cAMP Receptor-Like Protein That Is Important for Actin Organization and Panicle Development

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Mitochondria have been shown to associate with the actin cytoskeleton (Olyslaegers and Verbelen, 1998). In our drug experiments, mitochondria behaved in a manner almost identical to peroxisomes (data not shown).…”

Section: Effect Of the Myosin Inhibitors On Peroxisome Movementmentioning

confidence: 99%

“…The involvement of myosin motors has, in most cases, been presented as a major possibility rather than a proven fact (Olyslaegers and Verbelen, 1998;Kandasamy and Meagher, 1999). The well-analyzed movement of Golgi units in plants has relied on the use of two drugs, NEM and BDM, for associating myosin motors with Golgi motility (Boevink et al, 1998;Nebenfü hr et al, 1999).…”

Section: Peroxisome Movement May Not Involve Myosin Motors and Be An mentioning

confidence: 99%

“…These experiments suggested that, although peroxisome motility is actin based, it does not seem to involve myosin motors. To test whether this observation is peroxisome specific or is generally true for other organelles that display an actin-based motility, we assessed the motility of mitochondria (Olyslaegers and Verbelen, 1998). Transgenic plants carrying GFP-labeled mitochondria (Logan and Leaver, 2000) were used.…”

Section: Peroxisome Movement May Not Involve Myosin Motors and Be An mentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous Visualization of Peroxisomes and Cytoskeletal Elements Reveals Actin and Not Microtubule-Based Peroxisome Motility in Plants,

2002

View full text Add to dashboard Cite

Peroxisomes were visualized in living plant cells using a yellow fluorescent protein tagged with a peroxisomal targeting signal consisting of the SKL motif. Simultaneous visualization of peroxisomes and microfilaments/microtubules was accomplished in onion (Allium cepa) epidermal cells transiently expressing the yellow fluorescent protein-peroxi construct, a green fluorescent protein-mTalin construct that labels filamentous-actin filaments, and a green fluorescent proteinmicrotubule-binding domain construct that labels microtubules. The covisualization of peroxisomes and cytoskeletal elements revealed that, contrary to the reports from animal cells, peroxisomes in plants appear to associate with actin filaments and not microtubules. That peroxisome movement is actin based was shown by pharmacological studies. For this analysis we used onion epidermal cells and various cell types of Arabidopsis including trichomes, root hairs, and root cortex cells exhibiting different modes of growth. In transient onion epidermis assay and in transgenic Arabidopsis plants, an interference with the actin cytoskeleton resulted in progressive loss of saltatory movement followed by the aggregation and a complete cessation of peroxisome motility within 30 min of drug application. Microtubule depolymerization or stabilization had no effect.

show abstract

“…Only in certain types of differentiated mammalian cells have reticulate mitochondria been observed (Bakeeva et al, 1978;Bawa and Werner, 1988;BereiterHahn, 1990;Smirnova et al, 1998;Yaffe, 1999b). Similarly, higher plant mitochondria have been typically portrayed as round to sausage-like organelles (Olyslaegers and Verbelen, 1998;Logan and Leaver, 2000;Nebenfü hr et al, 2000;Arimura and Tsutsumi, 2002;Van Gestel and Verbelen, 2002;Logan et al, 2003;Arimura et al, 2004;Logan, 2006a). Although some studies report on the presence of reticulate mitochondria in unperturbed eggs (Kuroiwa et al, 1996(Kuroiwa et al, , 2002 and vascular cell types (Gamalei and Pakhomova, 1981), most of the documented examples of reticular or disc-shaped mitochondria come from mitochondrial mutants (Arimura and Tsutsumi, 2002;Logan et al, 2003) or from cells subjected to experimental perturbations such as low oxygen pressure (Bereiter-Hahn, 1990;Van Gestel and Verbelen, 2002;Logan, 2006a), prolonged cell culturing (Rohr, 1978), or protoplasting (Sheahan et al, 2004(Sheahan et al, , 2005.…”

Section: Reticular Mitochondria Have Not Been Observed In Unperturbedmentioning

confidence: 99%

The Mitochondrial Cycle of Arabidopsis Shoot Apical Meristem and Leaf Primordium Meristematic Cells Is Defined by a Perinuclear Tentaculate/Cage-Like Mitochondrion

2008

View full text Add to dashboard Cite

Plant cells exhibit a high rate of mitochondrial DNA (mtDNA) recombination. This implies that before cytokinesis, the different mitochondrial compartments must fuse to allow for mtDNA intermixing. When and how the conditions for mtDNA intermixing are established are largely unknown. We have investigated the cell cycle-dependent changes in mitochondrial architecture in different Arabidopsis (Arabidopsis thaliana) cell types using confocal microscopy, conventional, and threedimensional electron microscopy techniques. Whereas mitochondria of cells from most plant organs are always small and dispersed, shoot apical and leaf primordial meristematic cells contain small, discrete mitochondria in the cell periphery and one large, mitochondrial mass in the perinuclear region. Serial thin-section reconstructions of high-pressure-frozen shoot apical meristem cells demonstrate that during G1 through S phase, the large, central mitochondrion has a tentaculate morphology and wraps around one nuclear pole. In G2, both types of mitochondria double their volume, and the large mitochondrion extends around the nucleus to establish a second sheet-like domain at the opposite nuclear pole. During mitosis, approximately 60% of the smaller mitochondria fuse with the large mitochondrion, whose volume increases to 80% of the total mitochondrial volume, and reorganizes into a cage-like structure encompassing first the mitotic spindle and then the entire cytokinetic apparatus. During cytokinesis, the cage-like mitochondrion divides into two independent tentacular mitochondria from which new, small mitochondria arise by fission. These cell cycle-dependent changes in mitochondrial architecture explain how these meristematic cells can achieve a high rate of mtDNA recombination and ensure the even partitioning of mitochondria between daughter cells.Mitochondria are the principal source of ATP energy in eukaryotic cells. Although they are often portrayed as static, oval or rod-shaped organelles that sometimes exhibit a branched configuration, studies of living cells carried out over the past 30 years have demonstrated that they are among the most plastic organelles of cells in terms of form and distribution (Calvayrac et al

show abstract

Improved staining of F‐actin and co‐localization of mitochondria in plant cells

Cited by 69 publications

References 21 publications

Rice TUTOU1 Encodes a Suppressor of cAMP Receptor-Like Protein That Is Important for Actin Organization and Panicle Development

Rice TUTOU1 Encodes a Suppressor of cAMP Receptor-Like Protein That Is Important for Actin Organization and Panicle Development

Simultaneous Visualization of Peroxisomes and Cytoskeletal Elements Reveals Actin and Not Microtubule-Based Peroxisome Motility in Plants,

The Mitochondrial Cycle of Arabidopsis Shoot Apical Meristem and Leaf Primordium Meristematic Cells Is Defined by a Perinuclear Tentaculate/Cage-Like Mitochondrion

Contact Info

Product

Resources

About