IMPROVED TECHNIQUES FOR LABORATORY REARING OF THE SPINY BOLLWORM, Earias insulana (BOISDUVAL) (LEPIDOPTERA: NOCTUIDAE)

Amer, Amer; Atlam, El-Sayed; Raslan,

doi:10.21608/jppp.2010.86724

Cited by 3 publications

(3 citation statements)

References 5 publications

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“…The clothes with the deposited eggs were transferred to a convenient glass jars. The newly hatched larvae were reared on modified artificial diet according to Rashad and Ammar (1985) and Amer et al (2010).…”

Section: Methodsmentioning

confidence: 99%

Effect of Sublethal Dose of Hexaflumuron on the Ovary and Egg Maturation of Spiny Bollworm, Earias insulana (Boisd.)

El‐Sayed¹

2015

J. of Entomology

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The effect of LC 20 of hexaflumuron on some biological aspects, egg maturation and ovary of the spiny bollworm, Earias insulana (Boisd.) were studied under controlled conditions of 26±1EC and 75±5 RH. The calculated LC 20 and LC 50 values of hexaflumuron were 17.42 and 72.75 ppm. The treatment newly hatched larvae with LC 20 (17.42 ppm) elongated larval and pupal duration compared with control. Treatment significantly reduced mean numbers of deposited eggs and hatchability percentage compared with control. Hexaflumuron reduced number of immature eggs after emergence (20.56 eggs/ovariol) compared with 27.05 eggs/ovariol for untreated. No mature eggs were present in the female moths ovarioles after emergence of treated and untreated. Mature eggs were found after 36 h and 3 days in untreated and treated, respectively. The highest numbers of mature eggs 3.63 and 6.32 eggs/ovariol were recorded after four days from emergence in treated and untreated, respectively. Hexaflumuron reduced the ovarian weight and length, size of basal eggs and number of spermatophore/female compared with untreated. The LC 20 of hexaflumuron reduced significantly larval content of total carbohydrate, lipid and soluble protein.

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Section: Methodsmentioning

confidence: 99%

Effect of Sublethal Dose of Hexaflumuron on the Ovary and Egg Maturation of Spiny Bollworm, Earias insulana (Boisd.)

El‐Sayed¹

2015

J. of Entomology

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“…The newly hatched larvae of the spiny bollworm used in this study was obtained from a culture maintained for many generations at Bollworms Research Department, Plant Protection Research Institute, Sharkia branch on artificial diet have been described by Rashad and Ammar ( 1985 ), Rashad et al ( 1993 ) and Amer et al ( 2010 ) . Nine generations of insect were reared on each tested diets before beginning recording the experiment observations.…”

Section: Insectmentioning

confidence: 99%

ECONOMIC ARTIFICIAL DIETS FOR REARING SPINY BOLLWORM, Earias insulana (BOISD.) (LEPIDOPTERA: NOCTUIDAE)

Amer

2015

Journal of Plant Protection and Pathology

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The present investigation was conducted to evaluate the use of four artificial diets that don't contain agar and less expensive for mass rearing the spiny bollworm as compared to the agar-based artificial diet. These four diets A, B, C and D contained dried active yeast, ascorbic acid, sorbic acid, methyl parahydroxy benzoate, starch, liquid milk, vitamin mixture and formaldehyde 34-38%, except diet A not containing starch. In addition to the essential ingredient in diet A is kidney beans and wheat grated, diet B is kidney beans and soybeans flower , diet C is kidney beans and diet D is kidney beans and yellow lentils .The obtained results showed that the mean larval and pupal durations were affected significantly by diets A & B and nonsignificantly by diets C & D as compared with control diet .The longest larval and pupal periods were on control diet and shortest were on diet A .Weight of larvae and pupae reared on diets A, B, C and D were significantly higher than those of the larvae and pupae reared on control diet .The larval fed on the diet A gave the highest larval and pupal weights, while those fed on control diet gave the lowest weights .The highest larval and pupal mortality percentages were on control diet followed by diets C, B, D and A. The lowest pupation and emergence percentages were on control diet and the highest were on diet A .The highest number of deposited eggs and hatchability percentage were observed on diet A followed by diets B, D and C and the lowest were on control diet .The growth index and fitness index were highest on diet A and the lowest were on control diet. Moreover, the total cost of ingredients reduced by 5911 , 43.88 , 43.75 and 45.24% per 1000 g. diet of diets A, B, C and D, respectively as compared to the agar-based control diet.

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“…One hundred full grown larvae of E. insulana was obtained from a culture maintained for many generation at Bollworms Research Department, Plant Protection Research Institute, Sharkia branch on artificial diet have been described by Rashad and Ammar (1985), Rashad et al (1993) and Amer et al (2010).…”

Section: Laboratory Strainmentioning

confidence: 99%

INFLUENCE OF LARVAL FOOD ON NUMBER OF SPERMATOPHORES, FECUNDITY AND SOME BIOCHEMICAL PARAMETERS OF THE SPINY BOLLWORM, Earias insulana (BOISD.)

Amer

El-Sayed

2015

Journal of Plant Protection and Pathology

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Experiments were carried out to study the effect of larval food types (cotton bolls, okra pods and artificial diet) on number of spermatophores, fecundity and total soluble protein, lipid and carbohydrate contents of the spiny bollworm, Earias insulana (Boisd.). The obtained results showed that the larvae reared on artificial diet gave the highest weight, while, those collected from cotton bolls gave the lowest weight. The lowest pupal mortality percentage and the highest adult emergence percentages, number of eggs/female and hatchability percentages recorded for the larvae obtained from artificial diet followed by those collected from okra pods and cotton bolls. Pupal duration, pre & post-oviposition periods and male longevity were differed insignificantly as affected by feeding larvae on okra pods, cotton bolls and artificial diet. The newly emerged female moths from larvae collected from cotton bolls were recorded the lowest mean number of the eggs/ovariol and spermatophores, while, those emergence from larvae obtained from artificial diet was recorded the highest one. The highest value of total soluble protein, lipid and carbohydrate contents were recorded in larvae obtained from artificial diet followed by those collected from okra pods and cotton bolls.

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IMPROVED TECHNIQUES FOR LABORATORY REARING OF THE SPINY BOLLWORM, Earias insulana (BOISDUVAL) (LEPIDOPTERA: NOCTUIDAE)

Cited by 3 publications

References 5 publications

Effect of Sublethal Dose of Hexaflumuron on the Ovary and Egg Maturation of Spiny Bollworm, Earias insulana (Boisd.)

Effect of Sublethal Dose of Hexaflumuron on the Ovary and Egg Maturation of Spiny Bollworm, Earias insulana (Boisd.)

ECONOMIC ARTIFICIAL DIETS FOR REARING SPINY BOLLWORM, Earias insulana (BOISD.) (LEPIDOPTERA: NOCTUIDAE)

INFLUENCE OF LARVAL FOOD ON NUMBER OF SPERMATOPHORES, FECUNDITY AND SOME BIOCHEMICAL PARAMETERS OF THE SPINY BOLLWORM, Earias insulana (BOISD.)

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