Improved techniques for particle size determination by quasi-elastic light scattering

Morrison, Ian D.; Grabowski, E. F.; Herb, C. A.

doi:10.1021/la00064a016

Cited by 162 publications

(86 citation statements)

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“…The distribution was obtained by the NNLS method using an alpha value, which is a function parameter of the fitting sensitivity of the raw data. 46 We used a fixed alpha value to measure the polydispersity. All of distributions were calculated by the value of 0.01.…”

Section: Calculation Of the Distribution Of Various Proteins From Dlsmentioning

confidence: 99%

Polydispersity as a Parameter for Indicating the Thermal Stability of Proteins by Dynamic Light Scattering

et al. 2010

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Section: Calculation Of the Distribution Of Various Proteins From Dlsmentioning

confidence: 99%

Polydispersity as a Parameter for Indicating the Thermal Stability of Proteins by Dynamic Light Scattering

et al. 2010

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“…After that, it got the size gradually until 60 min. The non-negatively constrained least squares (NNLS) analysis 37 suggests that the micellization occurred by the intermolecular aggregation. ,,,, ,,,,,,, ,…”

Section: Resultsmentioning

confidence: 99%

Micelle Formation of “Nonamphiphilic” Poly(vinylphenol-co-styrene) Random Copolymers by Hydrogen Bond Cross-linking by α,ω-Diamine

Yoshida

Hironaka

2004

Polym J

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ABSTRACT:Well-defined poly(vinylphenol-co-styrene) random copolymers (P(VPh-r-St)) were prepared by living radical polymerization mediated by 4-methoxy-2,2,6,6-tetramethylpiperidine-1-oxyl. The copolymers showed no micellization in 1,4-dioxane, the nonselective solvent. Dynamic light scattering demonstrated that the copolymers formed micelles in the presence of ,!-diamine. The copolymer with the molecular weight of M n ¼ 8690 and the VPh/St unit ratio of 0.50/0.50 produced the micelles with ca. 90 nm as a hydrodynamic radius at 0.2 of the molar ratio of 1,4-butanediamine (BDA) to the VPh unit. It was found that the micellization occurred by intermolecular aggregation through hydrogen bond cross-linking between the VPh units by BDA, because the hydrodynamic radius and the aggregation number of the micelles increased with an increase in the copolymer concentration. The hydrodynamic radius of the micelles decreased with increasing the molecular weight of the copolymer, supporting the micellization by the intermolecular aggregation. And further, the micellar size also decreased with a decreasing the VPh ratio in the copolymer at the molecular weight constant.KEY Many useful micelles consist of macromolecules. 12-14Recently, the importance of the macromolecular micelles has been increasing because of the structural stability and the environmental safety. There are a number of publications on micelles prepared from amphiphilic block 15-22 and graft copolymers. 23,24 An increase in the number of publications on them has been accelerated since well-defined block and graft copolymers came to be prepared more easily by living radical polymerization techniques than by living ionic polymerization. [25][26][27] Micelles for amphiphilic random copolymers have also been well-studied on the basis of the advantage of convenient procedure for preparing the random copolymers over block and graft copolymers. 28-32While many amphiphilic copolymers are utilized for preparing micelles, ''nonamphiphilic'' copolymers attract considerable attention in recent years because of the advantages in the molecular design. We have already released publications on the micelle formation of a nonamphiphilic block copolymer consisting of poly(vinylphenol) and polystyrene (PVPh-b-PSt). 33,34The copolymer forms the micelles in the presence of ,!-diamine through hydrogen bond cross-linking in a nonselective solvent. We found that poly(vinylphenol-co-styrene) random copolymers (P(VPh-r-St)) showed a great difference from the PVPh-b-PSt diblock copolymer in the micellization by ,!-diamine. This paper describes the micelle formation of the nonamphiphilic P(VPh-r-St) random copolymers by ,!-diamine. EXPERIMENTAL MeasurementsGel permeation chromatography (GPC) was performed with a HLC-802A instrument by Tosoh Corp. equipped with RI and UV detectors. Two polystyrene gel columns, Tosoh TSK gel G4000H 8 and G2000H 8 , were used with THF as the eluent at 42 C. 1 H NMR spectra were obtained with a Bruker ARX-500 NMR spectrometer by Bruker Co. Light scattering exp...

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“…An aliquot of 25 l was freshly filtered (0.02 m Whatman Anodisc (Whatman, UK)) into the measurement cell. One hundred readings were recorded, and the data were analyzed using the non-negatively constrained least squares method (29) incorporated into the DYNAMICS version 5.20.05. The analysis is based on the Stokes-Einstein equation (D T ϭ k b T/6 P i (SV)R H ), under the assumption of Brownian motion.…”

Section: Methodsmentioning

confidence: 99%

dUTPase and Nucleocapsid Polypeptides of the Mason-Pfizer Monkey Virus Form a Fusion Protein in the Virion with Homotrimeric Organization and Low Catalytic Efficiency

Barábas

Rumlová

Erdei

et al. 2003

Journal of Biological Chemistry

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Improved techniques for particle size determination by quasi-elastic light scattering

Cited by 162 publications

References 0 publications

Polydispersity as a Parameter for Indicating the Thermal Stability of Proteins by Dynamic Light Scattering

Polydispersity as a Parameter for Indicating the Thermal Stability of Proteins by Dynamic Light Scattering

Micelle Formation of “Nonamphiphilic” Poly(vinylphenol-co-styrene) Random Copolymers by Hydrogen Bond Cross-linking by α,ω-Diamine

dUTPase and Nucleocapsid Polypeptides of the Mason-Pfizer Monkey Virus Form a Fusion Protein in the Virion with Homotrimeric Organization and Low Catalytic Efficiency

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