Improved therapeutic effects on diabetic foot by human mesenchymal stem cells expressing MALAT1 as a sponge for microRNA-205-5p

Zhu, Lingyan; Zhong, Qian; Yang, Tianlun; Xiao, Xiangwei

doi:10.18632/aging.102562

Cited by 35 publications

(32 citation statements)

References 28 publications

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“…83 Zhu et al overexpressed MALAT1 in MSCs. 84 When the modified MSCs were transplanted into wound tissues in STZ-induced diabetic mice, higher vessel density was detected, which reflected promotion of the angiogenesis phase. Further research showed that MALAT1 played its role by downregulating the expression of miR-205-5p.…”

Section: Proliferation Stagementioning

confidence: 99%

Emerging Roles of Long Non-Coding RNAs in Diabetic Foot Ulcers

Yan

Chen

Yang

et al. 2021

DMSO

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Diabetes mellitus is one of the most widespread metabolic diseases in the world, and diabetic foot ulcer (DFU), as one of its chronic complications, not only causes a large amount of physiological and psychological pain to patients but also places a tremendous burden on the entire economy and society. Despite significant advances in knowledge on the mechanism and in the treatment of DFU, clinical practice is still not satisfactory, and our understanding of its cellular and molecular pathogenesis is far from complete. Fortunately, progress in studying the roles of long non-coding RNAs (lncRNAs), which play important regulatory roles in the expression of genes at multiple levels, suggests that we can apply them in the early diagnosis and potential targeted intervention of DFU. In this review, we briefly summarize the current knowledge regarding the functional roles and potential mechanisms of reported lncRNAs in regulating DFU.

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Section: Proliferation Stagementioning

confidence: 99%

Emerging Roles of Long Non-Coding RNAs in Diabetic Foot Ulcers

Yan

Chen

Yang

et al. 2021

DMSO

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“… 27 lncRNAs play an epigenetic regulatory role by regulating methylation and histone modification; 16 , 28 they also interact with RNA molecules or protein complexes for transcriptional regulation 8 , 28 , 29 and can act as a molecular sponge of miRNAs to regulate downstream target genes for post-transcriptional regulation. 30 , 31 , 32 In addition, lncRNAs can also affect protein expression and interfere with protein binding. 33 , 34 , 35 , 36 , 37 Recent studies have shown that lncRNAs are key regulators of cell proliferation, differentiation, apoptosis, and other cell biological processes, thereby regulating wound healing ( Figure 3 ).…”

Section: Ncrnamentioning

confidence: 99%

The progress, prospects, and challenges of the use of non-coding RNA for diabetic wounds

Wei

Liu

et al. 2021

Molecular Therapy - Nucleic Acids

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Chronic diabetic wounds affect the quality of life of patients, resulting in significant social and economic burdens on both individuals and the health care system. Although treatment methods for chronic diabetic wounds have been explored, there remains a lack of effective treatment strategies; therefore, alternative strategies must be explored. Recently, the abnormal expression of non-coding RNA in diabetic wounds has received widespread attention since it is an important factor in the development of diabetic wounds. This article reviews the regulatory role of three common non-coding RNAs (microRNA [miRNA], long non-coding RNA [lncRNA], and circular RNA [circRNA]) in diabetic wounds and discusses the diagnosis, treatment potential, and challenges of non-coding RNA in diabetic wounds. This article provides insights into new strategies for diabetic wound diagnosis and treatment at the genetic and molecular levels. ncRNA ncRNA refers to a type of RNA that does not encode protein. In the past, it has been shown to participate in a variety of biological processes and play important transcriptional and post-transcriptional regulatory functions. 18 ncRNAs are divided into two categories based on the length of the RNA chain; lncRNA have lengths greater than 200 nucleotides, and small ncRNAs (snRNA) have lengths less than 200 nucleotides. snRNAs can be subdivided into miRNAs and circRNAs. 19 miRNAs inhibit the expression of target genes by binding to the 3 0 untranslated region (3 0 UTR) of the target mRNA. 20 The miRNA gene is processed and cleaved by RNA polymerase II (Pol II) enzyme and endonuclease Drosha III to produce pre-miRNA, which is

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“…1 Diabetic foot ulcers (DFUs) are a serious complication of this metabolic disease that arises as a consequence of impaired wound healing, resulting in secondary infections and the potential need for limb amputation, thus negatively impacting patient quality of life. 2 Many efforts have been made to explore the mechanistic basis for DFU onset in order to improve patient healing activity. 3 In one study, the lncRNA H19/miR-152-3p/PTEN axis was shown to function as a critical regulator of wound repair in individuals with diabetes, indicating that it may be a viable therapeutic target in the context of DFU development.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Circulating Exosomal miRNA-20b-5p Accelerates Diabetic Wound Repair

Chen

Wang

2021

IJN

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Purpose: Efficient approaches to reliably improving wound healing in diabetic patients remain to be developed. Exosomes are nanomaterials from which therapeutically active microRNAs (miRNAs) can be isolated. In the present report, we therefore isolated circulating exosome-derived miRNAs from patients with diabetes and assessed the impact of these molecules on wound healing. Patients and Methods: Exosomes were isolated from the serum of control or diabetic patients (Con-Exos and Dia-Exos, respectively), after which the effects of these exosomes on cellular activity and wound healing were assessed. Results: We determined that miR-20b-5p was overexpressed in Dia-Exos and that it functioned by impairing wound repair by suppressing vascular endothelial growth factor A (VEGFA) expression. Consistent with such a model, the administration of Dia-Exos or this miRNA both in vivo and in vitro was sufficient to slow wound repair. Conclusion: Dia-Exos exhibit significant increases in miR-20b-5p relative to Con-Exos, and this miRNA can be transferred into HSFs wherein it can suppress VEGFA expression and thereby slow the process of wound healing.

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Improved therapeutic effects on diabetic foot by human mesenchymal stem cells expressing MALAT1 as a sponge for microRNA-205-5p

Cited by 35 publications

References 28 publications

Emerging Roles of Long Non-Coding RNAs in Diabetic Foot Ulcers

Emerging Roles of Long Non-Coding RNAs in Diabetic Foot Ulcers

The progress, prospects, and challenges of the use of non-coding RNA for diabetic wounds

Inhibition of Circulating Exosomal miRNA-20b-5p Accelerates Diabetic Wound Repair

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