Improved Tissue Solubilization for Atomic Absorption.

Jackson, A. J.; Michael, L. W.; Schumacher, H. J.

doi:10.1021/ac60320a001

Cited by 3 publications

(3 citation statements)

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“…In the present study, thc optimal fixation and processing time for iron visualization was 10% buffered formalin for 24 hr. The intense histochemical reaction for iron in the white matter is consistent with quantitative studies reporting the majority of iron in thc brain is associated with the myelin fraction (Hallgren and Sourdnder, 1958;Jackson et al, 1972;Rajan et al, 1976). The distribution of the iron-positive cells in "patches" within the subcortical white matter is an apparent novel observation.…”

Section: Discussionsupporting

confidence: 84%

Cellular distribution of transferrin, ferritin, and iron in normal and aged human brains

Connor

Menzies

Martin

et al. 1990

J of Neuroscience Research

455

307

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The iron transport protein, transferrin, and the iron storage protein ferritin were examined immunohistochemically along with iron in a number of brain regions from normal and aged humans. Two age groups were examined: a middle-aged group (28-49 years), and an older group (60-90 years). Transferrin, ferritin, and iron are found throughout all brain regions examined, predominantly in the perikaryal cytoplasm of cells that are small and round, fitting the description of oligodendrocytes. These cells are present in the optic nerve and in both the gray and white matter of the cerebral cortex, cerebellum, and olfactory bulb in both age groups. Ferritin is also found in microglial cells in the gray matter of most of these brain regions. In the subcortical regions examined (corpus striatum, hippocampus, amygdala), in addition to oligodendrocytes, astrocytes can frequently be observed that contain transferrin, ferritin, and iron. There is an age-related alteration in cell labeling: astrocytes in both gray and white matter contained transferrin in the oldest age group, whereas in the younger group the subcortical transferrin immunoreactivity was confined mostly to oligodendrocytes. Ferritin in the subcortical brain regions is also present in astrocytes but is primarily confined to those in the gray matter, even in the oldest age group. Iron is found predominantly in oligodendrocytes, although a few iron-positive astrocytes and microglia can be identified. These results indicate that (1) normally oligodendrocytes contain much of the iron and iron-binding proteins found in the brain; and (2) an increase in age is associated with altered cellular distribution of iron-binding proteins, but the altered distribution is specific to glial cells. These results suggest glial cells may have previously undescribed functions related to metal regulation and sequestration.

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Section: Discussionsupporting

confidence: 84%

Cellular distribution of transferrin, ferritin, and iron in normal and aged human brains

Connor

Menzies

Martin

et al. 1990

J of Neuroscience Research

455

307

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“…The method to be described uses Soluene-1 00 (Packard Scientific), a quaternary ammonium base, to solubilize tissues. The use of this agent has previously only been described for the estimation of zinc, copper, iron and manganese (Jackson, Michael & Schumacher, 1972). This new method of assay has been used to study the distribution of lithium, magnesium and sodium in rat brain after administration of lithium in the diet for periods ranging from 2 to 42 days.…”

Section: Introductionmentioning

confidence: 99%

The Distribution of Lithium, Sodium and Magnesium in Rat Brain and Plasma After Various Periods of Administration of Lithium in the Diet

Bond

Brooks²,

Judd³

1975

British J Pharmacology

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1The tissue solubilizer Soluene-100 provides an efficient and easy means of preparing small amounts of rat tissue for cation analysis. 2 Administration of lithium ions to rats for two days to 42 days by the addition of lithium chloride to the diet at a concentration of 30 mmol/kg dry weight results in (a) the uniform distribution of lithium throughout the brain at a concentration comparable to that found in plasma; (b) decrease in the brain sodium concentration; (c) a decrease in brain magnesium concentration and an increase in plasma magnesium concentration; (d) no change in brain water content.3 The inclusion of LiCl in the diet at a concentration of 30 mmol/kg dry food gives consistent and predictable plasma and brain levels of lithium in the rat without the occurrence of serious side effects over periods of up to 42 days.

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“…Partial digestion to produce carbon-aceous slurries has also been successfully applied to the analysis, by inductively coupled plasmasatomic emission spectrometry (ICP-AES), of a series of standard reference materials of biological origin (Fagioli et al, 1986). Various alkylammonium hydroxide formulations have been used extensively to solubilize tissue (Hansen and Bush, 1967;Jackson et al, 1972;Murthy et al, 1973;Uchida et al, 1992), particularly those of zoological origin.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Digestion−High-Pressure Homogenization Prior to Slurry Introduction Graphite Furnace Atomic Absorption Spectrometry for Selenium Determination in Plant and Animal Tissues

Chen

Marshall

1999

J. Agric. Food Chem.

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Homogenization using a new flat valve homogenizer in combination with enzymatic digestion with a crude protease was investigated as a means of releasing Se compounds from zoological and botanical matrixes prior to slurry introduction GFAAS. Timed trials with four zoological certified reference materials (CRMs), three botanical reference materials (RMs), and a food crop indicated that Se release into 5% (v/v) ethanol-0.03 M TRIS containing 20 mg of protease was quantitative after homogenization or became quantitative within 1 h of digestion at 60 degrees C. For each of the zoological RMs (whole egg powder, dogfish muscle, and dogfish liver), three passes through the homogenizer in the presence of protease provided a quantitative release of selenium, and incubation with the enzyme was not necessary. No separation of the Se between the liquid phase and the particulate phase was evident even after several days of subsequent storage at 4 degrees C. The botanical matrixes (three milled wheat RMs and a rapeseed sample) were more resistant to selenium release and required up to 1 h of digestion with protease at 60 degrees C. Alternatively, 10 passes through the homogenizing valve (in the presence of the enzyme) resulted in the quantitative release of analyte.

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Improved Tissue Solubilization for Atomic Absorption.

Cited by 3 publications

References 0 publications

Cellular distribution of transferrin, ferritin, and iron in normal and aged human brains

Cellular distribution of transferrin, ferritin, and iron in normal and aged human brains

The Distribution of Lithium, Sodium and Magnesium in Rat Brain and Plasma After Various Periods of Administration of Lithium in the Diet

Enzymatic Digestion−High-Pressure Homogenization Prior to Slurry Introduction Graphite Furnace Atomic Absorption Spectrometry for Selenium Determination in Plant and Animal Tissues

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