Improved tolerance toward fungal diseases in transgenic Cavendish banana (Musa spp. AAA group) cv. Grand Nain

Vishnevetsky, Jane; White, Thomas L.; Palmateer, Aaron J.; Flaishman, Moshe A.; Cohen, Yuval; Elad, Y.; Velcheva, Margarita; Hanania, Uri; Sahar, N.; Dgani, Oded; Perl, Avihai

doi:10.1007/s11248-010-9392-7

Cited by 86 publications

(45 citation statements)

References 53 publications

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“…Many publications on transgenic field trials report data at single locations, replicated, on average, over three seasons [15], [16], [17], [18], [19], [20], [21]. To provide a comprehensive view of Bs2 field performance, we report seven trials in two locations on studies comparing (i) disease resistance relative to a set of genotypes with varying levels of resistance and (ii) disease resistance and yield relative to widely grown commercial cultivars.…”

Section: Discussionmentioning

confidence: 99%

Transgenic Resistance Confers Effective Field Level Control of Bacterial Spot Disease in Tomato

Horváth¹,

et al. 2012

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We investigated whether lines of transgenic tomato (Solanum lycopersicum) expressing the Bs2 resistance gene from pepper, a close relative of tomato, demonstrate improved resistance to bacterial spot disease caused by Xanthomonas species in replicated multi-year field trials under commercial type growing conditions. We report that the presence of the Bs2 gene in the highly susceptible VF 36 background reduced disease to extremely low levels, and VF 36-Bs2 plants displayed the lowest disease severity amongst all tomato varieties tested, including commercial and breeding lines with host resistance. Yields of marketable fruit from transgenic lines were typically 2.5 times that of the non-transformed parent line, but varied between 1.5 and 11.5 fold depending on weather conditions and disease pressure. Trials were conducted without application of any copper-based bactericides, presently in wide use despite negative impacts on the environment. This is the first demonstration of effective field resistance in a transgenic genotype based on a plant R gene and provides an opportunity for control of a devastating pathogen while eliminating ineffective copper pesticides.

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Section: Discussionmentioning

confidence: 99%

Transgenic Resistance Confers Effective Field Level Control of Bacterial Spot Disease in Tomato

Horváth¹,

et al. 2012

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“…A triple gene construct from heterologous sources viz. (i) endochitinase gene ThEn - 42 from Trichoderma harzianum , (ii) grape stilbene synthase ( StSy ), and (iii) superoxide dismutase Cu, Zn - SOD from tomato were used to transform Grand Nain banana (Vishnevetsky et al, 2011). Repeated field trials of this transgenic exhibited tolerance not only to Sigatoka but also to ‘Gray mold/ blossom blight’ – another fungal disease of banana caused by Botrytis cinerea .…”

Section: Disease Epidemics and Resistance In Cultivated Bananamentioning

confidence: 99%

Translating the “Banana Genome” to Delineate Stress Resistance, Dwarfing, Parthenocarpy and Mechanisms of Fruit Ripening

Dash

Rai

2016

Front. Plant Sci.

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Evolutionary frozen, genetically sterile and globally iconic fruit “Banana” remained untouched by the green revolution and, as of today, researchers face intrinsic impediments for its varietal improvement. Recently, this wonder crop entered the genomics era with decoding of structural genome of double haploid Pahang (AA genome constitution) genotype of Musa acuminata. Its complex genome decoded by hybrid sequencing strategies revealed panoply of genes and transcription factors involved in the process of sucrose conversion that imparts sweetness to its fruit. Historically, banana has faced the wrath of pandemic bacterial, fungal, and viral diseases and multitude of abiotic stresses that has ruined the livelihood of small/marginal farmers’ and destroyed commercial plantations. Decoding structural genome of this climacteric fruit has given impetus to a deeper understanding of the repertoire of genes involved in disease resistance, understanding the mechanism of dwarfing to develop an ideal plant type, unraveling the process of parthenocarpy, and fruit ripening for better fruit quality. Further, injunction of comparative genomics will usher in integration of information from its decoded genome and other monocots into field applications in banana related but not limited to yield enhancement, food security, livelihood assurance, and energy sustainability. In this mini review, we discuss pre- and post-genomic discoveries and highlight accomplishments in structural genomics, genetic engineering and forward genetic accomplishments with an aim to target genes and transcription factors for translational research in banana.

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“…The currently technology for banana transformation uses ECS cultures initiated form either scalps or immature male flower as starting plant material (Table 21.6), which have been applied to a range of banana cultivars and genotypes in which new genes (trans or cis) are successfully introduced. These approaches that include the expression of genes encoding plant, fungal, or bacterial hydrolytic enzymes Remy et al (1998) Rasthali ( (Vishnevetsky et al 2011), or the production of edible vaccine against hepatitis B (Kumar et al 2005) are examples of topics in Musa research.…”

Section: Banana Genetic Transformation Using Ecs Culturesmentioning

confidence: 99%

Somatic Embryogenesis in Banana, Musa ssp.

Escobedo-GraciaMedrano

Enríquez-Valencia

Youssef

et al. 2016

Somatic Embryogenesis: Fundamental Aspects and Applications

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In Musa (Musaceae family), as for other angiosperms, somatic embryo formation from somatic cells exemplify a distinctive phenomenon of plant cell developmental plasticity. Somatic embryogenesis (SE) through embryogenic cell suspension (ECS) cultures is an important milestone method for accelerating bananas' mass-propagation due to its high regeneration potential, and serves as powerful cellular tool for its non-conventional improvement. Protocols for SE have been standardized for several genotypes of wild Musa species (having AA and/or BB genomes), dessert (AA, AB and AAA), cooking (ABB), and plantain (AAB) bananas using different types of explants; however, in some cases, the protocols are limited by the low embryo germination and plant conversion rates. Therefore, efforts are needed to understand the physiological, biochemical, and genetic processes underpinning banana embryo development (zygotic and somatic), in order to inaugurate robust SE protocols with high rates of embryo germination and plant conversion. Here, we present an overview of the general progress in banana plant regeneration through somatic embryogenesis. Background InformationBananas and plantains, collectively known as bananas, belong to the genus Musa (Family: Musaceae); these monocotyledonous giant herbs are among the most important horticultural crops widely distributed throughout the humid tropical and

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Improved tolerance toward fungal diseases in transgenic Cavendish banana (Musa spp. AAA group) cv. Grand Nain

Cited by 86 publications

References 53 publications

Transgenic Resistance Confers Effective Field Level Control of Bacterial Spot Disease in Tomato

Transgenic Resistance Confers Effective Field Level Control of Bacterial Spot Disease in Tomato

Translating the “Banana Genome” to Delineate Stress Resistance, Dwarfing, Parthenocarpy and Mechanisms of Fruit Ripening

Somatic Embryogenesis in Banana, Musa ssp.

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