Neuromuscular electrical stimulation (NMES) is the action of therapeutic electrical stimulation on muscle tissue in order to contract the muscle and consequently improve the muscle status. Objective: To evaluate the muscle damage stemming from isometric muscle contraction induced by NMES of low frequency (30 Hz) and high frequency (100 Hz). Methods: Experimental crossover study, randomized, unblinded. The study included 10 male college students, age 24.4 ± 6.0 years, weight 77.1 ± 11.8 kg, height 176.1 ± 5.6 cm, and BMI of 24.8 ± 3 4 kg/m vs. 366.6 ± 84.1 IU, ns). The perception of contractile efficiency decreased significantly (p = 0.016) in the 100 Hz Protocol. Conclusion: Both the low-frequency NMES (30 Hz) and the high-frequency (100 Hz) elevate blood markers of muscle damage, most strikingly at the higher frequency. However, the achieved values reflect a normal response to a moderate-intensity exercise.