Improvement in symbiotic efficiency of chickpea (Cicer arietinum) by coinoculation of Bacillus strains with Mesorhizobium sp. Cicer

Abstract

Rhizobacteria belonging to Bacillus sp. were isolated from the rhizosphere of chickpea (Cicer arietinum). Ten Bacillus strains were studied for their antifungal activity, effect on seedling emergence and plant growth promotion. Two Bacillus strains CBS127 and CBS155 inhibited the growth of all the four pathogenic fungi tested on nutrient agar medium plates in vitro. Seed inoculation with different Bacillus strains showed stimulatory effect on root and shoot growth at 10 d of observation in comparison to control whereas four Bacillus strains CBS24, CBS127, CBS129 and CBS155 caused retardation of shoot growth at 10 d. Maximum nodule-promoting effect was observed with Bacillus strains CBS106, CBS127 and CBS155. The symbiotic effectiveness of Mesorhizobium sp. Cicer strain Ca181 was further improved on coinoculation with six Bacillus strains i.e. CBS9, CBS17, CBS20, CBS106, CBS127 and CBS155 at 80 d of plant growth under sterile conditions and shoot dry weight ratios increased 1.62 to 1.74 times those of Mesorhizobium-inoculated treatments, suggesting the usefulness of introduced rhizobacteria in improving crop productivity.

Co-inoculation of Mesorhizobium sp. and plant growth promoting rhizobacteria Pseudomonas sp. as bio-enhancer and bio-fertilizer in chickpea (Cicer arietinum L.)

“…These results suggest that increase in seed emergence in PGPR treatments might be due to induction of IAA production and phosphate solubilization. These results corroborated with the findings NS -Non significant of Sivaramaiah et al (2007) who also found stimulatory effect on seedling emergence at 10 days with two rhizobacteria Bacillus strains CBS 127 and CBS 155 in chickpea. The same results were reported by Kumar et al (2000) in which seed bacterization with both fluorescent Pseudomonas strains and Rhizobium and their combinations (co-inoculations) brought distinct crop enhancement in pea.…”

“…Maximum height was recorded with Meso+ PGPR 3 in both varieties (20.4 cm in desi PBG 1 and 21.9 cm in kabuli BG1053) followed by Meso + PGPR 2 and Meso+ LK884. This investigation has been found coherent between the result of Sivaramaiah et al (2007) who reported the enhancement in root and shoot length in agar plates at 10 days with Bacillus strains in chickpea. Improvement in plant height with PGPR or Mesorhizobium sp alone or dual over control plants in both varieties of chickpea could be attributed to presence of phytohormone (IAA) which function as signal molecule in the regulation of plant development.…”

“…PGPR are able to modify nodule formation and biological nitrogen fixation (BNF) when they are coinoculated with rhizobia (Garcia et al 2004). Several mechanisms such as alteration in the composition of rhizosphere microorganisms, production of plant signaling compounds, bacteriocins, siderophores, plant growth hormones and improving availability of nutrients by rhizosphere microorganisms have been reported for synergism (Sivaramaiah et al 2007). Synergistic effect of P fluorescence and Bacillus sp.…”

Growth stimulation of clusterbean (Cyamopsis tetragonoloba) by coinoculation with rhizosphere bacteria and Rhizobium

“…These PGPR improve the plant growth through multitudinous factors viz. production of plant growth promoting substances and hormones (Ahmad et al, 2008;Malik and Sindhu, 2011;Liu et al, 2014), early colonization of root surfaces (Benziri et al, 2001), reducing ethylene level due to synthesis of 1-aminocyclopropane-1-carboxylate (ACC) deaminase (Glick, 2014;Chaudhary and Sindhu, 2015), enhancing the availability of nutrients (Sivaramaiah et al, 2007;Sindhu et al, 2010) and by synthesis of antibiotic and other pathogen-depressing substances such as siderophores, cyanide and hydrolytic enzymes (Voisard et al, 1989;Sindhu and Dadarwal, 2001) and stimulation of phytoalexins/flavonoid-like compounds in roots (Goel et al, 2001). The present study was planned with the objective to evaluate the plant growth promotion ability of different rhizobacterial isolates under chillum jar conditions.…”

Characterisation of Phosphate Solubilising Bacteria in Sandy Loam Soil Under Chickpea Cropping System

“…The reports on the efficacy of Bacillus sp. showed enhance nodulation, plant dry matter and grain yield on co-inoculation with Rhizobia in other legumes [33]. Bacterial strains isolated from the maize rhizosphere including Bacillus, Pseudomonas and Serratia were reported to improve the yield by 9-14% [34].…”