Improvement in the Intestinal Absorption of Soy Protein By Enzymatic Digestion to Oligopeptide in Healthy Adult Men

Abstract

In this study, we compared the intestinal absorption from di#erent sources with the same amino acid composition (protein, peptide, and amino acid mixture) by measuring changes in serum concentrations of various amino acids. Peptides used in this study were prepared by enzymatic digestion of ++S globulin (a major component of soy protein). Twelve normal male adults were asked to drink beverages containing +,./ g of the test sample, representing /ῌ suspension of each of the following three di#erent preparations : ++S globulin, ++S peptide, and an amino acid mixture of equivalent composition. Venous blood samples were collected from * to +2* min to measure free amino acid concentrations. The early slope of serum concentrations and values of area under the curves for each amino acid calculated from changes in serum amino acid concentrations were significantly greater for ++S peptide than for either ++S globulin or the amino acid mixture. This trend was especially remarkable for branched-chain amino acids and aromatic amino acids. Our results suggest that the ingestion of hydrolyzed soy protein results in faster and more e$cient absorption than the consumption of protein or amino acid mixtures in healthy adult men.

Changes in the antioxidant activity of loach (Misgurnus anguillicaudatus) protein hydrolysates during a simulated gastrointestinal digestion

“…Clemente (2000) also found that protein hydrolysates were better absorbed than whole proteins. Their works and others (Maebuchi et al, 2007) showed that the peptide transport system plays a major role in the absorption of protein and peptide digestive products.…”

Angiotensin I-converting enzyme inhibitory activity of low-molecular-weight peptides from Atlantic salmon (Salmo salar L.) skin

“…The results obtained indicated that two-step enzymatic hydrolysis followed by multistage separation was effective in producing short oligopeptides and in removing large peptides or undigested proteins. It was reported that oligopeptide mixtures prepared from proteins had improved absorption properties, high solubility, low viscosity, and low allergenicity associated with low molecular weight peptides (Maebuchi et al, 2007). Moreover, small activity peptides are better candidates than longer peptides to play a physiological role in vivo as they are less susceptible to undergo gastrointestinal hydrolysis (Gómez-Ruiz, Ramos, & Recio, 2007).…”

Stability of Angiotensin I-converting Enzyme Inhibitory Activity of Peptides Extracted from Dry-cured Jinhua Ham

“…At this stage, more than 60% of the amino acids were present as peptides. It has been reported that some small molecular-sized peptides are directly absorbed through the intestine by a peptide transport system which plays an important role in the digestion and the absorption of protein [31].…”