Improvement of Adenoviral Vectors for Human Gene Therapy

Vigne, Emmanuelle; Dedieu, Jean-François; Orsini, Cécile; Latta, Martine; Klonjkowski, Bernard; Prost, Edouard; Lakich, M. M.; Kremer, Eric J.; Denèfle, Patrice; Perricaudet, Michel; Yeh, Patrice

doi:10.1007/978-3-642-61028-8_11

Cited by 4 publications

(2 citation statements)

References 76 publications

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“…In contrast, fiber that protrudes outward from the capsid does not interact with the chromatography support. The retention time of adenovirus was essentially unchanged when a (Lys) 7 sequence (17) or a Vn4 ligand (18) was introduced at the C-terminus or in the HI loop of fiber, respectively (F. Blanche, unpublished), although these modifications resulted in a change in the overall charge of the fiber at pH 7.0 from Ϫ3.6 to ϩ2.5 and ϩ3.4, respectively. Second, about half of total iso-Asp that was labeled with protein L-isoaspartyl methyltransferase was located in the hexon.…”

Section: Discussionmentioning

confidence: 98%

Stabilization of Recombinant Adenovirus: Site-Directed Mutagenesis of Key Asparagine Residues in the Hexon Protein

Blanche¹,

Cameron²,

Somarriba³

et al. 2001

Analytical Biochemistry

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Section: Discussionmentioning

confidence: 98%

Stabilization of Recombinant Adenovirus: Site-Directed Mutagenesis of Key Asparagine Residues in the Hexon Protein

Blanche¹,

Cameron²,

Somarriba³

et al. 2001

Analytical Biochemistry

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“…Inflammation and cytotoxicity of the adenovirus-infect ed organ constitute a general paradigm described in several tissues; it was attributed to a host immune response against immunogenic viral proteins (Yang et al, 1994). New generations of adenovirus obtained by deletion of sequences encoding immunologic viral proteins appear to be less immunogenic (Vigne et al, 1996;Wang and Finer, 1996;Dedieu et al, 1998). The use of these deleted viruses could be an interesting approach to diminish the inflam matory response after lens extraction, which could be more attractive than a nonspecific postoperative antiinflammato ry treatment.…”

Section: Discussionmentioning

confidence: 94%

Adenovirus-Mediated Suicide Gene Transduction: Feasibility in Lens Epithelium and in Prevention of Posterior Capsule Opacification in Rabbits

Malecaze¹,

Couderc²,

Neuville³

et al. 1999

Human Gene Therapy

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The most common complication of cataract surgery is the development of posterior capsule opacification (PCO). Hyperplasia of the lens epithelium is one of the main cellular events following phacoemulsification, and has been found to be an important feature contributing to opacification of the posterior capsule. Adenoviral vector-mediated transfer is a suitable method for transducing the herpes simplex virus thymidine kinase gene (HSV-tk) into proliferating cells, allowing for the selective killing of these cells by ganciclovir (GCV) treatment. To determine the potential of gene transduction for lens epithelial cells, we studied the transduction of rabbit lens epithelial cells with adenoviral vectors containing either the Escherichia coli beta-galactosidase (lacZ) gene or the HSV-tk gene in vitro and in vivo in an experimental model of PCO. The efficiency of lacZ gene transfer in rabbit lens epithelial cells was at least 95% both in vitro and in vivo. In vivo transduction with HSV-tk adenoviral vector followed by GCV treatment significantly inhibited the development of PCO (p<0.001). These results suggest that adenoviral vector-mediated transfer of HSV-tk into the proliferating lens epithelial cells is feasible and may provide a novel therapeutic strategy for PCO.

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Identification, cloning and sequence analysisof the equine adenovirus 1 hexon gene

Reubel

Studdert

1997

Arch. Virol.

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Based on sequence homology with human adenovirus 2 (HAdV2), the hexon gene of equine adenovirus 1 (EAdV1) was identified. HindIII restriction fragments containing the hexon and other viral genes were cloned into the plasmids pUC19 and pBlueScript SK(-) and sequenced. The nucleotide sequence of the hexon gene was completely determined and partial sequence data were obtained for seven other EAdV1 genes. Amino acid (aa) sequence comparison with published adenovirus (AdV) proteins identified the genes for the IIIa, penton, pVII, PVI, 23K proteinase, DNA binding and 100K proteins. The eight EAdV1 genes appeared to be in the same relative order as homologous genes of other AdV. The EAdV1 hexon protein was encoded between the hexon-associated pVI upstream and the 23K proteinase gene downstream and comprised 2742 nucleotides which translated into 913 aa. Similar to other members of the genus Mastadenovirus the EAdV1 hexon yielded two highly conserved genome segments at the N- and C-termini which flanked intermediate variable and hypervariable regions. The majority of the residue differences between EAdV1 and other AdV hexons occurred in two loops that are known for other AdV to protrude from the surface of the nucleocapsid. Amino acid comparisons with other AdV hexons revealed highest homology with HAdV12 hexon with 72% identical and 83% functionally similar residues, followed by bovine AdV3 hexon with 71% identities and 82% functional residue conservation. Phylogenetic analysis suggested that EAdV1 and other AdV do not have an immediate common ancestor.

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Improvement of Adenoviral Vectors for Human Gene Therapy

Cited by 4 publications

References 76 publications

Stabilization of Recombinant Adenovirus: Site-Directed Mutagenesis of Key Asparagine Residues in the Hexon Protein

Stabilization of Recombinant Adenovirus: Site-Directed Mutagenesis of Key Asparagine Residues in the Hexon Protein

Adenovirus-Mediated Suicide Gene Transduction: Feasibility in Lens Epithelium and in Prevention of Posterior Capsule Opacification in Rabbits

Identification, cloning and sequence analysisof the equine adenovirus 1 hexon gene

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