Improvement of amperometric glucose biosensor by the immobilization of FcCD inclusive complex and carbon nanotube

Abstract

A novel glucose biosensor was constructed by using ferrocene-carbonyl-beta-cyclodextrin (FcCD) inclusive complex as electron-transfer mediator and carbon nanotubes (CNTs) as electron-transfer promoter. FcCD inclusive complex and glucose oxidase (GOx) were covalently bonded to CNTs by poly-l-lysine (PLL) to fabricate a glucose biosensor. The electrocatalytic oxidation of glucose at the biosensor occurred at low potential below 200 mV, avoiding the interference of the main interfering substances in real samples containing a 5-times higher concentration of l-cysteine, ascorbic acid, and uric acid. The biosensor showed fast response for glucose. A broad linear range of glucose concentration from 1.0 x 10(-5) to 2.90 x 10(-3) M was obtained and the detection limit was 2.2 x 10(-6) M.

A Bayesian Whittaker–Henderson smoother for general‐purpose and sample‐based spectral baseline estimation and peak extraction

“…and s 2 S are the variances. We argue that eliminating only the noise below the baseline [29] may lead to a biased estimate of a baseline. Theoretically, noise is commonly assumed to occur randomly on either side of a baseline.…”

“…To indicate whether a peak should be extracted for a wave number, a t vector is commonly used [29][30][31]…”

“…To adapt the WH algorithm to baseline estimation for a spectrum with signals or peaks that significantly deviate from the baseline, the fidelity was revised by excluding these peaks [29][30][31]:…”

“…We propose a Bayesian Whittaker-Henderson (BWH) smoother in this paper. The algorithm is developed based on the earlier work on revised fidelity [29,30] and the Bayesian interpretation of WH approach. [27] Our algorithm extends these important contributions by introducing hierarchical priors to the variances of the fidelity and the smoothness.…”

A new approach for noninvasive transdermal determination of blood uric acid levels

“…Due to the usually low concentration of uric acid that can be extracted transdermally, biosensors for this purpose should ideally have high sensitivity. Many researchers have focused on the use of nanoparticles, 20,21 carbon nanotubes, 22,23 electrontransfer mediators, 24,25 bimetallic materials, 26,27 bienzymes, 22,23 and array configurations 28 to enhance the sensitivity of biosensors. To the best of our knowledge, there have been no reports of the use of a combination of the above approaches to enhance biosensor sensitivity.…”