Improvement of antibiotic titers fromStreptomyces bacteria by interactive continuous selection

Butler, Philip R.; Brown, Marie; Oliver, Stephen G.

doi:10.1002/(sici)1097-0290(19960120)49:2<185::aid-bit7>3.0.co;2-m

Cited by 49 publications

(35 citation statements)

References 28 publications

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“…It has been reported that micro-organisms can be experimentally endowed with tolerance to different stress conditions through 'evolutionary engineering', a term introduced for the first time by Butler et al (1996) to indicate an experimental approach that uses the principles of adaptive evolution to drive the selection of organisms with desired phenotypic traits. While 'metabolic engineering' implies the rational modification of the cellular metabolic pathways (Bailey, 1991), evolutionary engineering relies on the onset of spontaneous or experimentally induced random mutations followed by recombination and continuous evolution of large populations over many generations (Sauer, 2001).…”

Section: Introductionmentioning

confidence: 99%

Amplification of the CUP1 gene is associated with evolution of copper tolerance in Saccharomyces cerevisiae

et al. 2012

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Section: Introductionmentioning

confidence: 99%

Amplification of the CUP1 gene is associated with evolution of copper tolerance in Saccharomyces cerevisiae

et al. 2012

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“…The use of industrial strains of S. griseus would produce many times the yields achieved here (Butler et al, 1996). In addition, although commercially purified chitin was used here for convenience, a lower cost substrate would be partially purified chitin generated by lactic acid fermentation of shellfish wastes (Zakaraia et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Production of streptomycin from chitin using Streptomyces griseus in bioreactors of different configuration

Meanwell

Shama

2008

Bioresource Technology

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• This is a journal article. Rushton impellers and operated at high speed was used, the yield of streptomycin (3.1 mg/l) was the highest observed and occurred at approximately 500 hours. Cultivation of S. griseus in a bioreactor stirred at low speed by a U-shaped paddle resulted in a lower yield of streptomycin (1.8 mg/l) but this was achieved in a shorter period of time (400 hours). Increasing the concentration of chitin from 5 to 10 % w/v had no significant effect on either of these two parameters. The use of a novel vertical basket bioreactor in which the chitin flakes were contained within a wire mesh basket and were gently fluidised by air, enabled comparatively high yields of streptomycin (2.8 mg/l) in the relatively short time of 300 hours.

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“…The most direct way of achieving this would be to increase the air flowrate to the basket which would have the effect of increasing the fluidisation intensity. Moreover, future studies of this type would need to be conducted using industrial strains of S. griseus which produce very much higher yields of streptomycin [27] in order to establish whether a process based on the vertical basket bioreactor could compete with the conventional method used to produce streptomycin. …”

Section: Discussionmentioning

confidence: 99%

Chitin in a dual role as substrate for Streptomyces griseus and as adsorbent for streptomycin produced during fermentation

Meanwell¹,

Shama²

2006

Enzyme and Microbial Technology

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Streptomycin production from chitin by Streptomyces griseus was compared using two different types of bioreactor. The first was equipped with a combined U-shaped paddle and Rushton impeller. Also employed was a bioreactor of novel design in which the chitin was contained in a wire mesh basket that was totally submerged in a liquid salts medium. During operation the chitin was gently fluidised by air admitted into the basket. Fermentation was continued in both bioreactors until maximum antibiotic titres were achieved whereupon operation was interrupted to allow the streptomycin adsorbed to the chitin substrate to be extracted into pH 3.0 buffer before continuing fermentation of the same batch of chitin a second time. At a chitin concentration of 10 % (w/v) the highest streptomycin yields (c. 5.5 mg/L) were obtained using the stirred bioreactor, however growth occurred more rapidly in the vertical basket bioreactor.

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Improvement of antibiotic titers fromStreptomyces bacteria by interactive continuous selection

Cited by 49 publications

References 28 publications

Amplification of the CUP1 gene is associated with evolution of copper tolerance in Saccharomyces cerevisiae

Amplification of the CUP1 gene is associated with evolution of copper tolerance in Saccharomyces cerevisiae

Production of streptomycin from chitin using Streptomyces griseus in bioreactors of different configuration

Chitin in a dual role as substrate for Streptomyces griseus and as adsorbent for streptomycin produced during fermentation

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