Improvement of efficacy and decrement cytotoxicity of oxaliplatin anticancer drug using bovine serum albumin nanoparticles: synthesis, characterisation and release behaviour

Abstract

To sustained release of an anticancer drug, oxaliplatin (OX), a non-toxic and biocompatible nanocarrier based on bovine serum albumin (BSA) were synthesised by desolvation method and characterised using Fourier-transform infrared (FTIR) spectroscopy, field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM) and dynamic light scattering. The results showed that the BSA nanoparticles (BSANPs) with a mean magnitude of 187.9 ± 1.2 nm have spherical morphology with a smooth surface and a uniform distribution. Furthermore, OX was loaded onto the BSANPs and the loading was confirmed by FTIR, AFM and FESEM techniques. The percentage of encapsulation efficiency and drug loading were determined by absorption spectroscopy (UV-vis). The drug release studies showed that release of OX from BSANPs exhibited slower release rate. However, the release kinetics followed the first-order kinetic for both of them with the non-Fickian release behaviour. The electrochemical analysis showed stability of OX loaded onto the BSANPs (OX@BSANPs) and confirmed the diffusion mechanism. Furthermore, the results of MTT assay revealed increasing of normal cell viability and cancer cell death in the OX@BSANPs compared to only OX. It was shown that the BSANPs could be safely used as a biocompatible nanocarrier for the sustained release of OX. 2 Experimental 2.1 Materials BSA powder, phosphate buffered saline (PBS) tablet, dialysis bag (12 kDa), glutaraldehyde 25%, RPMI1640, 3-(4,5dimethylthialzol-a-yl)-2,5-diphenyltetrazolium bromide (MTT), foetal bovine serum (FBS), penicillin, dimethyl sulfoxide were obtained from Sigma-Aldrich Co., USA. OX anticancer drug powder was purchased from LG South Korea. Ethanol was purchased from Merck Chemical Co., Germany. HFFF2 and MCF7 cell lines were obtained from the cell bank of Pasteur institute in Tehran, Iran. All of the reagents were used as received. Deionised water was used throughout the experiment. 2.2 Methods The Fourier-transform infrared (FTIR) spectra of BSA, BSANPs and OX@BSANPs were carried out (Vector33 model, Bruker, USA) by KBr tablet and were scanned in the range of 400-40,000 cm −1. The hydrodynamic diameter and the distribution of the BSANPs were performed using dynamic light scattering (DLS)

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In vitrocytotoxicity and antibacterial activity of [Pd(AMTTO)(PPh3)2]: a novel promising palladium(ii) complex

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Cellulose/Exosome Nanocarrier for Improved Prolonged Release of 5-Fluorouracil: Preparation, Characterization, Drug Release Behavior and Cytotoxicity

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