Biodiesel usage is increasing steadily worldwide as the
push for
renewable fuel sources increases. The increased oxygen content in
biodiesel fuel is believed to cause decreased particulate matter (PM)
and increased nitrous oxides within its exhaust. The addition of fuel
additives to further increase the oxygen content may contribute to
even further benefits in exhaust composition. The aim of this study
was to assess the toxicity of 10% (v/v) diethylene glycol dimethyl
ether (DGDME) added as a biodiesel fuel additive. Primary human airway
epithelial cells were grown at the air-liquid interface and exposed
to diluted exhaust from an engine running on either grapeseed, bran,
or coconut biodiesel or the same three biodiesels with 10% (v/v) DGDME
added to them; mineral diesel and air were used as controls. Exhaust
properties, culture permeability, epithelial cell damage, and IL-6
and IL-8 release were measured postexposure. The fuel additive DGDME
caused a decrease in PM and nitrous oxide concentrations. However,
exhaust exposure with DGDME also caused decreased permeability, increased
epithelial cell damage, and increased release of IL-6 and IL-8 (p < 0.05). Despite the fuel additive having beneficial
effects on the exhaust properties of the biodiesel, it was found to
be more toxic.