Improvement of Full-Thickness Skin Graft Survival by Application of Vascular Endothelial Growth Factor in Rats

Abstract

The effect of vascular endothelial growth factor (VEGF) on skin graft survival was studied in rats. Models of skin grafting on muscle and periosteum were designed. For the study of skin grafting on muscle model, 32 rats were divided into 4 groups. VEGF was administrated systemically after skin graft placement intrafascially injected into the recipient bed at the time of graft placement and topically applied to the recipient bed at the time of graft placement. Control groups consisted of grafts placed on sites without systemic or local VEGF treatment. With the study of skin grafting on periosteum, 40 rats were divided into 4 groups. VEGF was systemically, intraperiosteally, and topically applied to the recipient bed. The control animals received no treatment. On the fifth postoperative day, the survival area of each skin graft was measured and the graft was harvested for histology with CD31 immunohistochemical staining. The results showed that in skin grafting on muscle model, the mean viable percentage of the skin graft was 66.1% +/- 10.2% in the group receiving systemic application of VEGF and 56.1% +/- 9.8% in the group receiving VEGF intrafascia injection. The survival percentages were significantly higher than those found in the control group (22.5% +/- 7.7%) and the group with VEGF topical application (30.8% +/- 4.1%). In skin grafting on periosteum, the group receiving VEGF intraperiosteum injection reached a survival percentage of 50.5% +/- 4.3%, significantly higher than the groups with VEGF systemic application (28.7 +/- 5.5%), VEGF topical application (32.5% +/- 4.8%), and the control (25.8% +/- 6.0%). Histology showed that sections taken from grafts with VEGF intrafascia and intraperiosteum treatment revealed angiogenesis. The data demonstrated that administration of VEGF into the either muscular or periosteal recipient beds for skin grafting can improve the skin graft survival.

Pre-vascularization Enhances Therapeutic Effects of Human Mesenchymal Stem Cell Sheets in Full Thickness Skin Wound Repair

“…Thus, the resulted scar tissue does not function like normal skin, as it does not contain the native skin structure such as hair follicles, sebaceous glands, and sensory nerve receptors 48. Although various approaches for improving skin graft survival and decreasing contraction have been tried 49-54, problems still exist in promoting early vascularization of grafts and alleviating inflammation 55, 56 to regenerate durable and functional skin. hMSCs have been employed for treatment of full thickness wounds in different animal models.…”

The Effects of Vascular Endothelial Growth Factor on Capsule Formation around Silicone Implant in Rats

“…VEGF is a major constituent of hESC-EPC CM, acting on the vascular endothelial cells and thereby promoting angiogenesis and increasing the vascular permeability in the peripheral tissue. VEGF has been reported to be effective in improving ischemic symptoms when administered at a lower dose [15][16][17]. We therefore designed the current experiment to examine the effects of a single administration of VEGF to improve ischemic symptoms.…”

On Aerobic Exercise and Behavioral and Neural Plasticity

“…VEGF is a growth factor that causes angiogenesis in both normal physiological conditions, such as the development of the corpus luteum during menstruation [174,175,176], and in pathological conditions such as exposure to hypoxia, or conditions of low oxygen tension [177], wound healing [178,179], and tumor growth and survival [145,180,181]. Furthermore, application of VEGF to brain slices in vitro or infusion of VEGF intravenously in an in vivo model results in significant increases in the vascularity of the brain [182].…”