Improvement of human sperm properties with platelet-rich plasma as a cryoprotectant supplement

Nabavinia, Maryam Sadat; Yari, Arash; Ghasemi-Esmailabad, Saeed; Gholoobi, Aida; Gholizadeh, Lida; Nabi, Ali; Lotfi, Marzieh; Moshrefi, Mojgan

doi:10.1007/s10561-022-10032-6

Cited by 10 publications

(4 citation statements)

References 31 publications

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“…The authors attribute such positive effects to the action exerted by growth factors on their receptors on the human sperm surface more than a direct antioxidant or ROS scavenging effect [51]. Minimal improving effects were observed with platelet-rich plasma [49,153] in a small number of samples. No studies so far have addressed the question of whether cryopreserved spermatozoa with enriched plasma retain the ability to fertilize and support embryo development.…”

Section: Possible Strategies To Prevent the Damage And New Approaches...mentioning

confidence: 99%

Cryopreservation of Human Spermatozoa: Functional, Molecular and Clinical Aspects

Traini

Vignozzi

Baldi

et al. 2023

IJMS

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Cryopreservation is an expanding strategy to allow not only fertility preservation for individuals who need such procedures because of gonadotoxic treatments, active duty in dangerous occupations or social reasons and gamete donation for couples where conception is denied, but also for animal breeding and preservation of endangered animal species. Despite the improvement in semen cryopreservation techniques and the worldwide expansion of semen banks, damage to spermatozoa and the consequent impairment of its functions still remain unsolved problems, conditioning the choice of the technique in assisted reproduction procedures. Although many studies have attempted to find solutions to limit sperm damage following cryopreservation and identify possible markers of damage susceptibility, active research in this field is still required in order to optimize the process. Here, we review the available evidence regarding structural, molecular and functional damage occurring in cryopreserved human spermatozoa and the possible strategies to prevent it and optimize the procedures. Finally, we review the results on assisted reproduction technique (ARTs) outcomes following the use of cryopreserved spermatozoa.

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Section: Possible Strategies To Prevent the Damage And New Approaches...mentioning

confidence: 99%

Cryopreservation of Human Spermatozoa: Functional, Molecular and Clinical Aspects

Traini

Vignozzi

Baldi

et al. 2023

IJMS

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show abstract

“…In addition to intracellular and extracellular CPAs, plasma macromolecules were used to improve the efficiency of sperm vitrification. As Nabavinia's study showed a positive effect on sperm motility, viability, and DNA integrity by adding a plateletrich plasma product, they concluded that plateletrich plasma has a beneficial impact on sperm (20).…”

Section: Discussionmentioning

confidence: 98%

Impact of cryoprotectant-free sperm vitrification in pulled-glass capillary on sperm parameters and DNA integrity: A lab trial study

Le,

Nguyen,

Thi Nguyen

et al. 2024

IJRM

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Background: Vitrification is a recently introduced yet widely applied assisted reproduction technique. So far, the effects of the chemicals and devices in vitrification on sperm motility and DNA integrity are still unclear. Objective: This study aimed to examine sperm quality, as determined by semen analysis and sperm DNA integrity when vitrified with or without cryoprotectant agents (CPAs) using pulled-glass capillaries. Materials and Methods: Between February and June 2020, 50 infertile men from the Hue Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Vietnam, were enrolled. Sperm samples, prepared using the swim-up technique, were divided into 2 groups: vitrification with CPAs (group 1) and without CPAs (group 2). Vitrified sperm samples were preserved in 10 μL pulled-glass capillaries. Motility, sperm membrane integrity, and the DNA fragmentation index were tested. Results: Sperm motility in vitrified media with CPAs (54.4 ± 11%) was statistically higher than in media without CPAs (51.14 ± 10.6%, p < 0.05). CPAs did not affect sperm membrane integrity or large halo ratio (71.34 ± 8.47 vs. 70.38 ± 8.11 and 50.84 ± 18.92 vs. 51.98 ± 19.44, respectively). Group 2 exhibited a lower DNA fragmentation index than group 1 after vitrification (14.2 ± 8.47 vs. 12.60 ± 9.03, p = 0.021). Conclusion: Using a pulled-glass capillary for sperm vitrification, the presence of CPAs in the vitrification medium resulted in higher progressive motility and lower DNA fragmentation index than the medium without CPAs. Key words: Vitrification, Sperm, DNA fragmentation, Cryoprotectant agents, Small volume.

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“…In the study conducted by Nabavinia et al, PRP was found to effectively improve the progressive motility and viability of the sperm while decreasing the count of immotile sperm. Sperm viability was notably higher in the PRP-treated samples, and PRP significantly reduced the rate of abnormal sperm [ 10 ]. A study conducted by Yan et al showed that adding 5% PRP to the frozen-thawed sperm improved sperm viability, motility, and membrane integrity.…”

Section: Discussionmentioning

confidence: 99%

Overcoming Male Factor Infertility: A Journey Through Assisted Reproductive Technology With Platelet-Rich Plasma Therapy

Ulhe,

Choudhary,

Shrivastava

et al. 2024

Cureus

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This case study presents a couple’s journey through assisted reproductive technology (ART) experiencing two failed in vitro fertilization cycles. The couple underwent a comprehensive examination, revealing the normal parameters for the female, but asthenoteratozoospermia in the male indicating high morphological defects and reduced sperm motility. Subsequently, intracytoplasmic sperm injection (ICSI) was planned. Despite retrieving six oocytes during ovum pickup (OPU), all blastocysts stopped growth on the second day, prompting a sperm chromatin test disclosing highly DNA-fragmented sperm. Platelet-rich plasma (PRP) therapy was initiated to improve sperm quality, along with frozen embryo transfer (FET). Sperm were incubated with PRP, yielding improved sperm motility and reduced sperm DNA fragmentation. OPU yielded five good-quality metaphase II (MII) oocytes, which were successfully fertilized with PRP-treated sperm, resulting in the formation of four blastocysts. These blastocysts were frozen and later used for FET, resulting in a positive pregnancy outcome and successful conception. This case highlights the importance of personalized intervention in addressing the infertility factor in males and achieving successful ART outcomes.

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Improvement of human sperm properties with platelet-rich plasma as a cryoprotectant supplement

Cited by 10 publications

References 31 publications

Cryopreservation of Human Spermatozoa: Functional, Molecular and Clinical Aspects

Cryopreservation of Human Spermatozoa: Functional, Molecular and Clinical Aspects

Impact of cryoprotectant-free sperm vitrification in pulled-glass capillary on sperm parameters and DNA integrity: A lab trial study

Overcoming Male Factor Infertility: A Journey Through Assisted Reproductive Technology With Platelet-Rich Plasma Therapy

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