Improvement of in vitro fertilization in buffalo by increasing the fertilizing capacity of spermatozoa

Elruby, Ahmed; Hussein, Mamdouh; Bedir, Wael; Montasser, Abdelmonem; Badr, M. A.; hegab, Abdelraouf; Zaabel, Samy

doi:10.5455/ajvs.265634

Cited by 1 publication

(1 citation statement)

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“…https://doi.org/10.17221/48/2022-VETMED Some capacitating agents are secreted in the female reproductive tract during in vivo fertilisation (Maitan et al 2022), but need to add these agents in the in vitro fertilisation (IVF) medium during in vitro conditions (Reckova et al 2015). These agents, heparin, caffeine, calcium ionophore and PHE (d-penicillin, hypotaurine, and epinephrine) have been used singly or combined with others (Waheed et al 2016;El-Ruby et al 2017). The different supplements used (the best results of different concentrations grouped in a study) for the IVM, IVF and IVC medium during IVEP in buffaloes are presented in Table 4.…”

Section: Adding Supplements To the Media Used In Ivepmentioning

confidence: 99%

Factors affecting the in vitro embryo production in buffalo (Bubalus bubalis): A review

Kumar¹,

MS²,

Silva³

et al. 2023

Vet. Med.

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Under natural and well-managed conditions, the buffalo has good reproductive and productive indices. However, in vitro embryo production (IVEP) has been used commercially to maximise the number of elite animals. In this species, several factors (donor management, in vitro culture medium, semen, in vitro conditions, embryo transfer) still affect the IVEP results. In addition, the cost of this technique is very high for this purpose. Therefore, more studies, as well as adequate plans, are needed to achieve this objective efficiently. In this review, we discussed the current commercial status, influencing factors (in vivo and in vitro), and the progress and future challenges of IVEP in buffalo. A total of 81 references were used from 1979 to 2022. The relevant data or literature were searched using the following databases: Google, ResearchGate, Science Alert, Science Direct and PubMed, using the following keywords: buffalo oocytes/COCs, buffalo embryos, pregnancy and calving or live birth rate after embryo transfer. The best maturation, cleavage and blastocyst rates in the in vitro production of buffalo embryos were 95.8, 75.2 and 33.4%, respectively. The pregnancy and live birth rates ranged from 22.2% to 43.5% and from 15.3% to 36.5%, respectively, after the transfer of fresh embryos produced in vitro to the recipients. This review will help to contextualise IVEP in buffaloes, as well as create an adequate plan for implementing IVEP in buffaloes.

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Section: Adding Supplements To the Media Used In Ivepmentioning

confidence: 99%