Improvement of in vitro oocyte maturation with lectin supplementation and expression analysis of Cx43, GDF-9, FGF-4 and Fibronectin mRNA transcripts in Buffalo (Bubalus bubalis)

Pandey, Alok; Gupta, Neelam; Gupta, S.C.

doi:10.1007/s10815-009-9314-x

Cited by 15 publications

(6 citation statements)

References 29 publications

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“…For this reason, an oocyte has more gap junction channels than other cells and may be influenced more by adjacent cells. Recent studies regarding oocyte maturation have focused on the role of gap junctions and their main component protein Cx43 (Pant et al ., 2005; Pandey et al ., 2009; Kempisty et al ., 2014). Compared with normal cells, which have no zona pellucidae, oocytes have transzonal projections through zona pellucidae (Palma et al ., 2012).…”

Section: Discussionmentioning

confidence: 99%

Localization and quantitative analysis of Cx43 in porcine oocytes during in vitro maturation

Lee

Hiradate

Hoshino

et al. 2015

Zygote

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Many studies of the main gap junction protein, Cx43, have been conducted in porcine oocyte research, but they have been limited to investigations of cumulus-oocyte complexes (COCs). In this study, we verified Cx43 not in COCs, but in porcine oocytes during maturation, and conducted a quantitative time course analysis. The location and dynamics of Cx43 were examined by immunocytochemistry and western blotting, respectively. COCs were cultured in NCSU23 medium and processed for immunocytochemistry and western blotting at 0, 14, 28, and 42 h after denuding. A Cx43 signal was detected on oolemmas, transzonal projections and the surface of zona pellucidae. Western blotting showed that Cx43 band density increased from 0 to 14 h, and gradually decreased thereafter. Our results clarified that Cx43 is localized in the ooplasmic membrane through zona pellucidae and its level changes over time during culture in porcine oocytes.

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Section: Discussionmentioning

confidence: 99%

Localization and quantitative analysis of Cx43 in porcine oocytes during in vitro maturation

Lee

Hiradate

Hoshino

et al. 2015

Zygote

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“…They are implicated in integrin-mediated cell adhesion, assembly of the extracellular matrix, and multi-modular protein structures [ 41 ]. It has been reported that Fibronectin affects cumulus expansion and polar body extrusion [ 42 ], so it regulates cell adhesion and plays a role in oocyte maturation. Plasminogen (PLG) and Kininogen-1 were the other two proteins with high degrees that were down-regulated and up-regulated in PCOS patients, respectively [ 41 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

Repurposing new drug candidates and identifying crucial molecules underlying PCOS Pathogenesis Based On Bioinformatics Analysis

et al. 2021

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Backgrounds Polycystic ovary syndrome affects 7% of women of reproductive ages. Poor-quality oocytes, along with lower cleavage and implantation rates, reduce fertilization. Objective This study aimed to determine crucial molecular mechanisms behind PCOS pathogenesis and repurpose new drug candidates interacting with them. To predict a more in-depth insight, we applied a novel bioinformatics approach to analyze interactions between the drug-related and PCOS proteins in PCOS patients. Methods The newest proteomics data was retrieved from 16 proteomics datasets and was used to construct the PCOS PPI network using Cytoscape. The topological network analysis determined hubs and bottlenecks. The MCODE Plugin was used to identify highly connected regions, and the associations between PCOS clusters and drug-related proteins were evaluated using the Chi-squared/Fisher's exact test. The crucial PPI hub-bottlenecks and the shared molecules (between the PCOS clusters and drug-related proteins) were then investigated for their drug-protein interactions with previously US FDA-approved drugs to predict new drug candidates. Results The PI3K/AKT pathway was significantly related to one PCOS subnetwork and most drugs (metformin, letrozole, pioglitazone, and spironolactone); moreover, VEGF, EGF, TGFB1, AGT, AMBP, and RBP4 were identified as the shared proteins between the PCOS subnetwork and the drugs. The shared top biochemical pathways between another PCOS subnetwork and rosiglitazone included metabolic pathways, carbon metabolism, and citrate cycle, while the shared proteins included HSPB1, HSPD1, ACO2, TALDO1, VDAC1, and MDH2. We proposed some new candidate medicines for further PCOS treatment investigations, such as copper and zinc compounds, reteplase, alteplase, gliclazide, Etc. Conclusion Some of the crucial molecules suggested by our model have already been experimentally reported as critical molecules in PCOS pathogenesis. Moreover, some repurposed medications have already shown beneficial effects on infertility treatment. These previous experimental reports confirm our suggestion for investigating our other repurposed drugs (in vitro and in vivo). Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s40199-021-00413-9.

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“…Many studies in recent years have shown significant expression of connexin 43 (Cx43) during both oocyte (50) and granulosa cells (30,31) in vitro primary culture. This protein (GJA1, gap junction associated protein, alpha 1, also known as Cx43) turns out to be the main constituent in the connections between individual GCs populations: mural-mural and mural-CCs (68).…”

Section: Role Of Connexins Gene Family In Reproductive Systems Processesmentioning

confidence: 99%

Potential of aquaporins and connexins in dogs and their relation to the reproductive tract

Kulus¹,

Wieczorkiewicz²,

Kulus³

et al. 2021

Medycyna Weterynaryjna

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The complexity of processes in the female reproductive system of mammals is extremely sophisticated. The overall relationship between the processes during the oestrus cycle in animals is quite well understood, but the molecular background of these processes still requires an in-depth analysis. Bitches are distinguished by a specific course of sexual cycle during which the oocyte matures after ovulation in the oviduct. Other species of mammals are characterized by maturation of the oocyte within the ovary. Acquisition of developmental competence by cumulus – oocyte complexes seems to be a process with a complex molecular background, and the key to understanding it may be the analysis of intercellular channels. Aquaporins and connexins are structural proteins that are built into the cell membrane. Their location is widespread in many body tissues. Recent years have shown that they exhibit significant expression in different parts of the mammalian reproductive system, although the number of studies on dogs is still negligible. This review paper presents the current state of knowledge of water channels and gap junction connections in different animal species, with particular focus on dogs, and also explores the role of aquaporins and connexins in the acquisition of reproductive competences.

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Improvement of in vitro oocyte maturation with lectin supplementation and expression analysis of Cx43, GDF-9, FGF-4 and Fibronectin mRNA transcripts in Buffalo (Bubalus bubalis)

Abstract: Mitogenic lectin supplemented maturation media improves oocyte quality for in vitro embryo production.

Cited by 15 publications

References 29 publications

Localization and quantitative analysis of Cx43 in porcine oocytes during in vitro maturation

Localization and quantitative analysis of Cx43 in porcine oocytes during in vitro maturation

Repurposing new drug candidates and identifying crucial molecules underlying PCOS Pathogenesis Based On Bioinformatics Analysis

Potential of aquaporins and connexins in dogs and their relation to the reproductive tract

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