Localization and quantitative analysis of Cx43 in porcine oocytes during in vitro maturation

Lee, Seung‐Hoon; Hiradate, Yuuki; Hoshino, Yumi; Ko, Yeoung-Gyu; Tanemura, Kentaro; Sato, Eimei

doi:10.1017/s0967199415000271

Cited by 8 publications

(6 citation statements)

References 35 publications

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“…Bars with different letters (a, b, and c) indicate significant differences, COC, cumulus-oocyte complexes; DAPI, 4′,6-diamidino-2-phenylindole; DMSO, dimethyl sulfoxide; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; SEM, standard error of mean; p < 0.05; ***p < .001, comparing the indicated groups oocyte maturation. In mammal COCs, Cx43 plays a major function on GJICs, which is primarily expressed in granulosa cells, theca cells, and the surface of zona pellucida (Lee et al, 2016;Sommersberg et al, 2000;Zhang et al, 2019). Similar to this finding, our results showed that Cx43 was abundantly observed in cumulus cells and the surface of zona pellucida.…”

Section: Discussionsupporting

confidence: 87%

A novel role of SIRT2 in regulating gap junction communications via connexin‐43 in bovine cumulus‐oocyte complexes

Liu

et al. 2020

Journal Cellular Physiology

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SIRT2, the predominantly cytosolic sirtuin, plays important role in multiple biological processes, including metabolism, stress response, and aging. However, the function of SIRT2 in gap junction intercellular communications (GJICs) of cumulus-oocyte complexes (COCs) is not yet known. The purpose of the present study was to evaluate the effect and underlining mechanism of SIRT2 on GJICs in COCs. Here, we found that treatment with SIRT2 inhibitors (SirReal2 or TM) inhibited bovine oocyte nuclear maturation. Further analysis revealed that SIRT2 inactivation disturbed the GJICs of COCs during in vitro maturation. Correspondingly, both the Cx43 phosphorylation levels and MEK/MER signaling pathways were induced by SIRT2 inhibition. Importantly, SIRT2-mediated Cx43 phosphorylation was completely abolished by treatment with MEK1/2 inhibitor (Trametinib). Furthermore, treatment with SIRT2 inhibitors resulted in the high levels of MEK1/2 acetylation. Functionally,

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Section: Discussionsupporting

confidence: 87%

A novel role of SIRT2 in regulating gap junction communications via connexin‐43 in bovine cumulus‐oocyte complexes

Liu

et al. 2020

Journal Cellular Physiology

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“…Previous studies have shown that CX43, an important protein involved in GJC, is strongly expressed in CCs enclosing mature oocytes that exhibit enhanced preimplantation development following in vitro fertilization or parthenogenesis (Blaha et al., 2017; Kim et al., 2017; Li et al., 2008). This is because CX43 facilitates the transportation of small molecules such as glucose and amino acids from CCs to the oocyte via transzonal projections during porcine oocyte maturation (S. Lee et al., 2016). Moreover, SDC4, a transmembrane heparin sulfate proteoglycan, exhibited higher expression in CCs enclosing completely matured oocytes (MII stage) compared to CCs enclosing incompletely matured oocytes (GV or GVBD stage) (Adriaenssens et al., 2011).…”

Section: Discussionmentioning

confidence: 99%

In vitro maturation using an agarose matrix with incorporated extracellular matrix proteins improves porcine oocyte developmental competence by enhancing cytoplasmic maturation

Park

Kim

Lee

et al. 2021

J Tissue Eng Regen Med

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Here, we present a novel in vitro maturation (IVM) system comprising an agarose matrix supplemented with extracellular matrix (ECM) proteins for enhanced maturation of immature oocytes within cumulus–oocyte complexes (COCs) derived from porcine medium antral follicles (MAFs). Immunocytochemical analyses of integrin subunit α2, α5, α6, β1, and β4 expression suggested that integrin α2β1, α5β1, α6β1, and α6β4 play pivotal roles in IVM of porcine immature oocytes. Combinatorial supplementation of fibronectin interacting with integrin α5β1, collagen interacting with integrin α2β1, and laminin interacting with integrin α6β1 and α6β4 to the agarose matrix had no significant effect on nuclear maturation. However, the number of parthenogenetic embryos that developed into blastocysts increased when oocytes were matured using agarose IVM matrices supplemented with fibronectin, collagen, or laminin. Furthermore, significant increases in cytoplasmic maturation‐related parameters (BMP15 level, cumulus cell expansion score, intra‐oocyte ATP level, and index of cortical granule distribution) were observed in COCs matured in vitro using ECM protein‐incorporated agarose matrices. Our data suggest that mature porcine oocytes with enhanced developmental competence and high‐quality cytoplasm can be generated via IVM using agarose matrices supplemented with fibronectin, collagen, or laminin.

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“…In mammal COCs, Cx43 is mainly expressed in granulosa cells, membrane cells, and zona pellucida surfaces. 44,52 Cx43coupling GJICs were necessary to maintain granulosa cell proliferation, 53 and the deficiency of Cx43 results in a retarded growth of oocytes. 16 The regulatory mechanism of Cx43 phosphorylation has been shown in the GJICs, which was important.…”

Section: ■ Discussionmentioning

confidence: 99%

17β-Estradiol Enhances Porcine Meiosis Resumption from Autophagy-Induced Gap Junction Intercellular Communications and Connexin 43 Phosphorylation via the MEK/ERK Signaling Pathway

Duan

Chen

et al. 2021

J. Agric. Food Chem.

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Estrogen and its analogues are ubiquitous in agricultural environments, with large biological functions of oocyte development. Gap junction intercellular communications (GJICs) are the structural basis in cumulus−oocyte complexes (COCs) and regulate oocyte maturation and developmental material transport through a number of pathways. This study mainly determines the effect and potential mechanism of estrogen (17β-estradiol) in regulating GJICs in porcine COCs. In our study, 17β-estradiol increased porcine nuclear maturation in a time-dependent manner. The analysis revealed that 17β-estradiol upregulated the autophagy in COCs during in vitro maturation. In contrast with the control, 17β-estradiol decreased GJICs in a time-dependent manner between cumulus cells and oocytes, while it was consistent with the control group at 24 h. Carbenoxolone (CBX) blocks GJICs as a negative control group used in our system. Autophagy inhibitor autophinib decreased oocyte maturation, and the reduced nuclear maturation treated with autophinib was abolished by 17β-estradiol. Besides, the upregulation effect of autophinib on GJICs and transzonal projections (TZPs) was decreased by 17β-estradiol. 17β-Estradiol could reduce serine 368 phosphorylation of connexin 43 (Cx43) protein by autophinib in porcine COCs. These results were dependent upon the MEK/ERK signaling pathway. Furthermore, 17β-estradiol-induced GJICs and Cx43 phosphorylation were inhibited by autophinib or the MEK/ERK pathway inhibitors (Trametinib and FR 180204), indicating that 17β-estradiol regulated GJICs through the MEK/ERK signaling pathway. In conclusion, 17β-estradiol improves the autophagy-mediated nuclear maturation with downregulating GJICs and TZPs in porcine COCs. Such an effect occurs by phosphorylation of Cx43, which was regulated via the MEK/ERK signaling pathway.

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Localization and quantitative analysis of Cx43 in porcine oocytes during in vitro maturation

Cited by 8 publications

References 35 publications

A novel role of SIRT2 in regulating gap junction communications via connexin‐43 in bovine cumulus‐oocyte complexes

A novel role of SIRT2 in regulating gap junction communications via connexin‐43 in bovine cumulus‐oocyte complexes

In vitro maturation using an agarose matrix with incorporated extracellular matrix proteins improves porcine oocyte developmental competence by enhancing cytoplasmic maturation

17β-Estradiol Enhances Porcine Meiosis Resumption from Autophagy-Induced Gap Junction Intercellular Communications and Connexin 43 Phosphorylation via the MEK/ERK Signaling Pathway

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