2011
DOI: 10.1016/j.mrgentox.2010.12.005
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Improvement of in vivo genotoxicity assessment: Combination of acute tests and integration into standard toxicity testing

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Cited by 96 publications
(59 citation statements)
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“…Recent work has, however, suggested that, at least in the liver, tissue cytotoxicity does not lead to increased DNA migration in the in vivo Comet assay [29,38]. Additionally, work by Rothfuss et al [29] demonstrated an increase in DNA damage in the in vivo liver Comet assay for rats exposed to 100 mg/kg-day of 2,6-DNT for 3 days or 33 mg/kg-day for 29 days [28,29].…”
Section: Discussionmentioning
confidence: 99%
“…Recent work has, however, suggested that, at least in the liver, tissue cytotoxicity does not lead to increased DNA migration in the in vivo Comet assay [29,38]. Additionally, work by Rothfuss et al [29] demonstrated an increase in DNA damage in the in vivo liver Comet assay for rats exposed to 100 mg/kg-day of 2,6-DNT for 3 days or 33 mg/kg-day for 29 days [28,29].…”
Section: Discussionmentioning
confidence: 99%
“…Nevertheless, since tissue-toxicity is more likely to be induced during an RDT study than an acute-dose study this issue might be more critical when performing the Comet assay integrated into an RDT study (Vasquez, 2010). A review of published (and some unpublished) in vivo Comet assay data with concurrent cytotoxicity analysis data of mainly non-genotoxic carcinogens recently performed by an IWGT Working Group did not provide evidence that cytotoxicity, by itself, generates increases in DNA migration resulting in false-positive findings (Rothfuss et al, 2011). Anyway, it is imperative to describe in study reports any confounding factors that may have an influence on the induction of comets, such as cytotoxicity or cell division.…”
Section: Impact Of Toxicitymentioning
confidence: 99%
“…Several designs for administration of substances in the in vivo Comet assay are described in the literature (Hartmann et al, 2003(Hartmann et al, , 2004Burlinson et al, 2007;Vasquez, 2010;Rothfuss et al, 2010Rothfuss et al, , 2011.…”
Section: Administration Of Dosesmentioning
confidence: 99%