Abstract-We propose a novel imaging system useful in dermatology, more precisely for the follow-up of patients with an increased risk of skin cancer. The system consists of a Pentium PC equipped with a RGB frame grabber, a 3-chip CCD camera controlled by the serial port and equipped with a zoom lens and a halogen annular light source.Calibration of the imaging system provides a way to transform the acquired images, which are defined in an unknown color space, to a standard well-defined color space called sRGB. sRGB has a known relation to the CIE 1 XYZ and CIE L * a * b * colorimetric spaces. These CIE color spaces are based on the human vision, and allow the computation of a color difference metric called CIE ∆E * ab , which is proportional to the color difference as seen by a human observer. Several types of polynomial RGB to sRGB transforms will be tried, including some optimized in perceptually uniform color spaces. The use of a standard and well-defined color space also allows meaningful exchange of images, e.g. in teledermatology.The calibration procedure is based on 24 patches with known color properties, and takes about 5 minutes to perform. It results in a number of settings called a profile which remains valid for tens of hours of operation. Such a profile is checked prior to acquiring images using just one color patch, and is adjusted on the fly to compensate for short-term drift in the response of the imaging system. Precision or reproducibility of subsequent color measurements is very good with ∆E * ab = 0.3 and ∆E * ab < 1.2. Accuracy compared to spectrophotometric measurements is fair with ∆E * ab = 6.2 and ∆E * ab < 13.3.