Al-Qur'an verses that are read with correct recitation and tartil have frequencies and wavelengths that can affect the brain with the aim of analyzing differences in the number of neurons in the cerebrum and cerebellum of Rattus norvegicus newborns between exposure to Surah Al-Fatiha and the control group. The experimental study used a pure experimental design (Posttest Only Control Group Design), the treatment was carried out in a soundproof box for 1 hour at night on the 9th day of pregnancy with an intensity of 65 dB. The cerebrum and cerebellum were prepared, stained by HE, calculated by 400x magnification, and then analyzed using appropriate statistics. Number of neurons average in the cerebrum was 167.75 and for the control group was 126.62. In cerebellum was 80.50 and 63.00 in the control group. There were a statistically higher number of neurons in the Al Fatiha group, with p=0.046 in the cerebrum and p=0.030 in the cerebellum. Conclusion: Sura Al Fatiha, during pregnancy, gave a higher number of newborn brain neurons compared with the control group.