Improvement of palmitate-induced insulin resistance in C2C12 skeletal muscle cells using Platycodon grandiflorum seed extracts

Yoon, Hyo Jeong; Bang, Myun‐Ho; Kim, Hoon; Imm, Jee-Young

doi:10.1016/j.fbio.2018.08.002

Cited by 15 publications

(14 citation statements)

References 38 publications

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“…Glucose uptake in muscle is mediated by insulin dependent IRS/PI3K/AKT and/or insulin independent AMPK signal transduction. In both cases, translocation of GLUT4, a major glucose transporter, translocates to the plasma [42]. The expression of GLUT4 and its plasma translocation was significantly suppressed in the diabetic state [43].…”

Section: Resultsmentioning

confidence: 99%

Characteristics and Glucose Uptake Promoting Effect of Chrysin-Loaded Phytosomes Prepared with Different Phospholipid Matrices

et al. 2019

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Chrysin-loaded phytosomes (CP) were prepared using either soya phosphatidylcholine (SPC) or egg phospholipid (EPL) by the solvent evaporation method. Different phospholipid matrices resulted in significant differences in size, mechanical property and solubility of the CP. The most stable CP was obtained with EPL at a molar ratio of 1:3 (chrysin: EPL, CEP-1:3). CEP-1:3 displayed an average size of 117 nm with uniform size distribution (polydispersity index: 0.30) and zeta potential of −31 mV. A significantly greater elastic modulus of CEP-1:3 (2.7-fold) indicated tighter packing and strong molecular bonding than those of CP prepared with SPC (CSP-1:3). X-ray diffraction and Fourier transform infrared spectroscopic analysis of CEP-1:3 confirmed molecular complexation. CEP-1:3 displayed a greater glucose uptake promoting effect than free chrysin and CSP-1:3 in muscle cells by stimulating gene expression of peroxisome proliferator-activated receptor γ and glucose transporter type 4. The results of the present study suggest that the phospholipid matrix used for the preparation of phytosomes critically influences their performance.

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Section: Resultsmentioning

confidence: 99%

Characteristics and Glucose Uptake Promoting Effect of Chrysin-Loaded Phytosomes Prepared with Different Phospholipid Matrices

et al. 2019

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“…Saponin obtained from Platycodon grandiflorum is effective in reducing scrotal spermatogenic damage and suppressing oxidative stress and apoptosis in mice (Leng et al., 2019); decreasing muscle damage from exercise (Kim et al, 2018); and supporting muscle protein synthesis and mitochondrial function (Kim et al, 2018). Extracts of Platycodon grandiflorum seeds and roots showed insulin resistance in skeletal muscle cell model (Yoon, Bang, Kim, & Imm, 2018). Fructan, a polysaccharide isolated from Platycodon grandiflorum , exerted prebiotic and immunomodulatory effects in six lactobacillus strains and intestinal cells, respectively (Pang et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

Platycodon grandiflorumroots: A comprehensive study on odor/aroma and chemical properties during roasting

et al. 2020

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Platycodon is a ubiquitous plant widely grown in Asia. This study investigated changes in odor/aroma associated sensory attributes and chemical properties in Platycodon grandiflorum roots upon roasting. Amino acid analysis, electronic tongue analysis, chemical property analysis, volatile compound analysis, GC‐olfactometry‐assisted sensory attributes, and electronic nose analysis were performed. In results, amino acid profiles showed diverse patterns. Electronic tongue analysis somewhat corresponded to the free amino acid profiles. Total phenolic content, antioxidant capacity, and browning intensity significantly increased up to 4 min and slightly decreased afterward. Various pyrazines relevant to roasted odor such as 3‐ethyl‐2, 5‐dimethyl‐pyrazine, and 2, 6‐dimethyl‐pyrazine were generated by roasting. In electronic nose analysis, positive odor parameter significantly increased and potential unpleasant odorants significantly decreased over time. This is believed to be the first study demonstrating overall insight on odor/aroma and chemical characteristics and utilizing objective sensory measures on roasted Platycodon grandiflorum roots for food applications. Practical applications This study will be utilized (a) to researchers and food companies who are interested in medicinal foods (b) to individuals and food industry that search for changes in sensory characteristics and chemical changes of foods induced by roasting, and (c) to farmers and crop producers who look for utilization and applications of Platycodon grandiflorum roots as food sources and ingredients.

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“…To date, most studies have highlighted bioactivities of PG root (PGR), with only a few scientific reports regarding PG seed extract (PGSE). In a previous study, we demonstrated that total phenolic and total flavonoid contents of PGSE are significantly higher than those of the root extract (PGRE) (Yoon et al, 2018). PGSE also yielded a greater glucose uptake promotion effect compared to PGSE in insulin resistance-induced C2C12 muscle cells (Yoon et al, 2018).…”

Section: Introductionmentioning

confidence: 88%

“…In a previous study, we demonstrated that total phenolic and total flavonoid contents of PGSE are significantly higher than those of the root extract (PGRE) (Yoon et al, 2018). PGSE also yielded a greater glucose uptake promotion effect compared to PGSE in insulin resistance-induced C2C12 muscle cells (Yoon et al, 2018). As part of an ongoing study exploring various bioactivities of PGSE, its anti-inflammatory activities and their underlying mechanisms were investigated using RAW 264.7 macrophages.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and anti-inflammatory activities of Platycodon grandiflorum seeds extract

Kim

Yoon

Lee

et al. 2020

CyTA - Journal of Food

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Antioxidant and anti-inflammatory effects of seeds and root extracts (PGSE vs. PGRE) prepared from Platycodon grandiflorum (PG) by ethanol extraction were evaluated. PGSE exhibited greater 2,2-diphenyl-l-picrylhydrazyl (DPPH•) and 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS•+) radical scavenging activities and more effectively inhibited production of nitric oxide (NO) and prostaglandin E 2 (PGE 2 ) compare to PGRE. The release of tumor necrosis factor-α (TNFα) was also decreased by 31% at 100 μg/mL of PGSE in LPS-stimulated RAW 264.7 macrophages. LPS-induced elevated expressions of inducible NO synthase, cyclooxygenase-2, and proinflammatory cytokines (interleukin (IL)-1β, IL-4, and IL-6) were significantly downregulated, in a dose-dependent manner, by PGSE treatment. The suppression of nuclear factor κB (NFκB) translocation and stimulation of heme oxygenase-1 induction were related to anti-inflammatory mechanism of PGSE. Based on these findings, PGSE has the potential to be used as a functional agent for the mitigation of inflammation related diseases.

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Improvement of palmitate-induced insulin resistance in C2C12 skeletal muscle cells using Platycodon grandiflorum seed extracts

Cited by 15 publications

References 38 publications

Characteristics and Glucose Uptake Promoting Effect of Chrysin-Loaded Phytosomes Prepared with Different Phospholipid Matrices

Characteristics and Glucose Uptake Promoting Effect of Chrysin-Loaded Phytosomes Prepared with Different Phospholipid Matrices

Platycodon grandiflorumroots: A comprehensive study on odor/aroma and chemical properties during roasting

Antioxidant and anti-inflammatory activities of Platycodon grandiflorum seeds extract

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