Improvement of patient stratification in human papilloma virus‐associated oropharyngeal squamous cell carcinoma by defining a multivariable risk score

Oberste, M; Riders, Armands; Abbaspour, Bektasch; Kerschke, Laura; Beule, Achim G.; Rudack, Claudia

doi:10.1002/hed.26822

Cited by 5 publications

(6 citation statements)

References 26 publications

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“…A continuous, diffuse staining for p16 within the cancer area of the tissue sections was considered positive, while a focal staining or no staining were considered negative. The cut-off of p16 positivity was considered a diffuse staining corresponding to 70% of p16 positive tumor cells [13]. IHC slides were evaluated by one pathologist blinded to any other clinical information or HPV DNA or RNA status.…”

Section: Resultsmentioning

confidence: 99%

“…The authors suggest that these factors could be used for a better stratification and management of this category of patients [35]. A research team from Lyon used p16/p53 algorithm to predict HPV tumor status in OPSCC, and authors from Germany concluded that tobacco, alcohol abuse, age, and gender should be added to the current UICC (The Union for International Cancer Control) staging system to improve the risk stratification in HPV+ OPSCC [13,36]. Korsten LHA et al from Amsterdam evaluated the health-related quality of life for HPV-positive OPSCC patients.…”

Section: Opscc Hpv Dna Positive P16 Risk Factorsmentioning

confidence: 99%

“…The study will continue till 2023, to better analyze if acute and late toxicities associated with chemoradiotherapy are a significant burden for OPSCC patients, and if there are any adequate strategies that could decrease treatment-associated morbidity [12]. De-intensifying treatment trials in HPV-associated OPSCC was analyzed by researchers from Germany and the USA [13,14].…”

Section: Introductionmentioning

confidence: 99%

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Understanding the Pattern of Oropharyngeal Cancers from North-East Romanian Patients

et al. 2021

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Background: Human papilloma virus (HPV) is acknowledged as a risk factor for oropharyngeal squamous cellular cancers (OPSCC), of which the dominant types are tonsillar (TSCC) and base of tongue cancer (BOTSCC). Objective: To assess the role of HPV in selected OPSCC cases, from Romanian patients by sensitive and complementary molecular assays. Material and Methods: Fifty-four formalin fixed paraffin embedded (FFPE) OPSCC samples were analyzed for HPV DNA by a PCR-based bead-based multiplex-assay. Thirty-four samples were tested for HPV RNA and for overexpression of p16INK4a by immunohistochemistry. Twenty samples were evaluated by Competitive Allele-Specific Taqman PCR (CAST-PCR) for fibroblast growth factor receptor 3 protein (FGFR3) status. Results: A total of 33.3% (18/54) OPSCC samples were positive for HPV DNA. HPV16 was the most frequent type (30%, 16/54); followed by HPV18 (3.7%, 2/54); and 1 sample (1.8%) was positive for both HPV16 and 18. HPV18 E6*I was detected in a HPV18 DNA-positive oropharynx tumor. Four samples positive for HPV16 were also positive for p16INK4a. All the tested samples were negative for FGFR3. Conclusions: The increased HPV16 prevalence is in line with similar studies and is a new confirmation that HPV16 is the most prevalent type in our country; supporting the potential benefit of prophylactic vaccines. Overall, there is no concordance between DNA and any of the two other analytes that are considered being markers of HPV-driven cancers. There is a need to explore novel screening strategies that could be broadly used in the clinical routine to initiate preventive measures.

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Section: Resultsmentioning

confidence: 99%

Section: Opscc Hpv Dna Positive P16 Risk Factorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Understanding the Pattern of Oropharyngeal Cancers from North-East Romanian Patients

et al. 2021

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“…Currently, there are various radiographic biomarkers for HNSC patient outcome, namely using PET-CT scans rather than clinical evaluation and CT alone (107)(108)(109)(110) and tumor volume (110)(111)(112)(113)(114)(115)(116)(117)(118)(119)(120)(121). However, knowledge of HPV status alone is also a prognostic biomarker for HNSC in its own right, indicating better survival compared to HPV(-) (122) Unfortunately, a subset of HPV(+) individuals do not display improved survival (11,13,(123)(124)(125)(126)(127)(128), and therefore, a need exists for criterion to further stratify this subset of HPV(+) patients for guiding treatment decisions. HPV(+) tumors have increased tumor immune infiltrates, which have also been shown to be positive for prognosis (57,68,(129)(130)(131).…”

Section: Discussionmentioning

confidence: 99%

Tumor Cell Extrinsic Synaptogyrin 3 Expression as a Diagnostic and Prognostic Biomarker in Head and Neck Cancer

Murphy

Tasoulas

Porrello

et al. 2022

Cancer Research Communications

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Over 70% of oropharyngeal head and neck squamous cell carcinoma (HNSC) cases in the United States are positive for human papillomavirus (HPV) yet biomarkers for stratifying oropharyngeal head and neck squamous cell carcinoma (HNSC) patient risk are limited. We used immunogenomics to identify differentially expressed genes in immune cells of HPV(+) and HPV(-) squamous carcinomas. Candidate genes were tested in clinical specimens using both quantitative RT-PCR and IHC and validated by IHC using the Carolina Head and Neck Cancer Study (CHANCE) tissue microarray of HNSC cases. We performed multiplex immunofluorescent staining to confirm expression within the immune cells of HPV(+) tumors, receiver operating characteristic (ROC) curve analyses, and assessed survival outcomes. The neuronal gene Synaptogyrin-3 (SYNGR3) is robustly expressed in immune cells of HPV(+) squamous cancers. Multiplex immunostaining and single cell RNA-seq analyses confirmed SYNGR3 expression in T cells, but also unexpectedly in B cells of HPV(+) tumors. ROC curve analyses revealed that combining SYNGR3 and p16 provides more sensitivity and specificity for HPV detection compared to p16 IHC alone. SYNGR3-high HNSC patients have significantly better prognosis with five-year OS and DSS rates of 60% and 71%, respectively. Moreover, combining p16 localization and SYNGR3 expression can further risk stratify HPV(+) patients such that high cytoplasmic, low nuclear p16 do significantly worse (Hazard Ratio, 8.6; P = 0.032) compared to patients with high cytoplasmic, high nuclear p16. SYNGR3 expression in T and B cells is associated with HPV status and enhanced survival outcomes of HNSC patients.

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“…Alcohol and nicotine consumption are known risk factors for the development of HNSCC and the human papilloma virus (HPV) is an established risk factor for OPSCC 2,3 . HPV‐positive OPSCC cases are characterized by a superior response to therapies and longer survival 4,5 . However, overall, the 5‐year survival rate for OPSCC and LSCC is still low and additional risk factors seem to play a crucial role in carcinogenesis and tumor microenvironment.…”

Section: Introductionmentioning

confidence: 99%

Effects of squamous cell carcinoma and smoking status on oropharyngeal and laryngeal microbial communities

Oberste,

Böse,

dos Anjos Borges

et al. 2023

Head & Neck

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BackgroundStill, little is known about microbial dysbiosis in oropharyngeal and laryngeal tissue as risk factor for development of local squamous cell carcinoma. The site‐specific microbiota at these regions in healthy and cancer tissue and their modulation by environmental factors need to be defined.MethodsThe local microbiota of cancer tissue and healthy controls was profiled by 16S rRNA gene amplicon sequencing and statistical analysis using 111 oropharyngeal and 72 laryngeal intraoperative swabs.ResultsOropharynx and larynx harbor distinct microbial communities. Clear effects of both smoking and cancer were seen in the oropharynx whereas effects in the larynx were minor.ConclusionThe distinct microbial communities at larynx and oropharynx partially explain why the effects of cancer and smoking were distinct at those sites. Thus, the use of microbiota supposed to mirror community changes in another target location should be avoided and more studies on the actual cancerous environment are necessary.

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Improvement of patient stratification in human papilloma virus‐associated oropharyngeal squamous cell carcinoma by defining a multivariable risk score

Cited by 5 publications

References 26 publications

Understanding the Pattern of Oropharyngeal Cancers from North-East Romanian Patients

Understanding the Pattern of Oropharyngeal Cancers from North-East Romanian Patients

Tumor Cell Extrinsic Synaptogyrin 3 Expression as a Diagnostic and Prognostic Biomarker in Head and Neck Cancer

Effects of squamous cell carcinoma and smoking status on oropharyngeal and laryngeal microbial communities

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