Improvement of peaks identification and dynamic range for bi-polar Single Particle Mass Spectrometer

Chudinov, A. V.; Li, Lei; Zhou, Zhen; Huang, Zhengxu; Gao, Wei; Yu, Jiajun; Никифоров, С. М.; Pikhtelev, A. R.; Bukharina, A. B.; Kozlovskiy, V. I.

doi:10.1016/j.ijms.2018.11.013

Cited by 10 publications

(14 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The TOF on the scale of the corrected mass spectrum is converted from old values T old to new values T new using the relation

T_{new} = a T_{old} + b,

where a and b are coefficients that provide the “best” superposition of the corrected mass spectrum peaks and the peaks of the reference spectrum. In this case, the coefficients a and b can be obtained by maximizing the function, which is, in fact, a scalar product of two mass spectra, as described earlier . However, this scale adjustment algorithm can be quite slow, because at each step, a scalar product of sufficiently large data arrays is calculated, and the best values of the parameters a and b are found using a nonlinear curve fitting algorithm, which is obviously inferior to the simple linear regression in computation speed.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Improvement of m/z accuracy for linear matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometer

Chudinov

et al. 2020

Rapid Comm Mass Spectrometry

Self Cite

View full text Add to dashboard Cite

Rationale: Linear matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) is widely used in analytical and biomedical applications. The use of delayed extraction increases the resolution, but the roughness of the matrix crystals and the misalignment of the target plate in the order of a few micrometers cause a substantial spread in the ion TOF values and a decrease in mass accuracy. Methods:The method of mass spectra correction based on the correlation of matrix fragment peaks in MALDI mass spectra was used. Experiments were performed using the MALDI-TOF instrument CMI-1600. SIMION 8.1 and MATLAB were used for ion motion simulations. Data analysis was done using the home-built custom-developed software and MATLAB.Results: It was shown that the peak position drift in the MALDI-TOF mass spectra depends linearly on the TOF in a wide mass range. While using the linear correction of the TOF scale, an increase in m/z accuracy of more than 10 times was achieved.The mass accuracy was limited by the resolution of the fast Analog-to-Digital Converter (ADC) used. Conclusions:It is expected that the proposed method will significantly increase the dynamic range, since it becomes possible to sum up corrected individual mass spectra without a significant loss of resolution. Timescale adjusting can be used for both linear TOF instruments and reflector systems of various configurations.

show abstract

“…The TOF on the scale of the corrected mass spectrum is converted from old values T old to new values T new using the relation

T_{new} = a T_{old} + b,

Section: Methodsmentioning

confidence: 99%

“…In this paper, we propose a method for compensating the jittering of peaks, associated both with the initial ion coordinate spread and with the distortions of the target plate geometry. The proposed method is based only on the analysis of the mass spectra obtained . This method can be used for recording and processing MALDI spectra.…”

Section: Introductionmentioning

confidence: 99%

Improvement of m/z accuracy for linear matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometer

Chudinov

et al. 2020

Rapid Comm Mass Spectrometry

Self Cite

View full text Add to dashboard Cite

show abstract

“…Unfortunately, in spite of resolution enhancement with this new technique, ion peak position is still very sensitive to initial ion coordinates and speed. Chudinov et al (2019) have demonstrated that the ion peak shifts in 208 Pb + and 147 Na(NO 3 ) − 2 could be varied in the range of ±10 ns and the ion start position could be varied in the range of ±150 µm. As a result, substantial peak jittering is observed when switching between mass spectra of each individual particle.…”

Section: Introductionmentioning

confidence: 99%

Development of an automatic linear calibration method for high-resolution single-particle mass spectrometry: improved chemical species identification for atmospheric aerosols

Zhu

Wang

et al. 2020

Atmos. Meas. Tech.

Self Cite

View full text Add to dashboard Cite

Abstract. The mass resolution of laser desorption ionization (LDI) single-particle aerosol mass spectrometry (SPAMS) is usually low (∼500), which has been greatly improved by the recent development of the delayed ion extraction technique. However, due to large fluctuations among LDI processes during each laser shot, accurate calibration of the mass-to-charge ratio for high-resolution SPAMS (HR-SPAMS) spectra is challenging. Here we developed an automatic linear calibration method to improve the accuracy of mass-to-charge (m∕z) measurement for single atmospheric aerosol particles. Laboratory-generated sea spray aerosol and atmospheric ambient aerosol were tested. After the calibration, the fluctuation ranges of the reference ions' (e.g., Pb+ and SO4+) m∕z reaches ±0.018 for sea spray aerosol and ±0.024 for ambient aerosol in average mass spectra. With such m∕z accuracy, the HR-SPAMS spectra of sea spray aerosol can easily identify elemental compositions of organic peaks, such as Cx, CxHy and CxHyOz. While the chemical compositions of ambient aerosols are more complicated, CxHy, CxHyOz and CNO peaks can also be identified based on their accurate mass. With the improved resolution, the time series of peaks with small m∕z differences can be separated and measured. In addition, it is also found that applying high-resolution data with enhanced mass calibration can significantly affect particle classification (identification) using the ART-2a algorithm, which classify particles based on similarities among single-particle mass spectra.

show abstract

“…Unfortunately the ion source cannot be built very small. But it is possible to switch off the electrical field by using a delay extraction technique (DE) Chudinov et al, 2019).…”

mentioning

confidence: 99%

“…In our previous works we have used the double exponential pulse delay extraction technique to improve the resolution of SPMS (Chudinov et al, 2019;Li et al, 2018).This solution provides resolution enhancement about 2-3 times over the broad mass range in comparison to the usual DC extraction. It is important, that in the delay extraction ion source particles pass the half of the ion source with no DC electrical field.…”

mentioning

confidence: 99%

Increase of the particle hit rate in laser single particle mass spectrometer by pulse delayed extraction technology

Chen¹,

Kozlovskiy²,

Du³

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

Abstract. Single particle mass spectrometer is the instrument providing a plenty of valuable information on chemical and physical parameters of individual particles in real time. One of the main performance criteria of the instrument is the efficiency of particles detection (hit rate). Most of SPMS instruments use DC extraction, when the stationary high voltage is applied to the extraction electrodes. As the aerosol particles initially carry a certain charge, they can be deflected by this electric field thus decreasing the hit rate. It was realized in this work, that the delay extraction technique can eliminate the stochastic dispersion of the particles beam caused by their deflection in the stationary electric field. As the result, the hit rate of the instrument can be significantly improved. Also, as the effect of deflection in the electric field is mass dependent, it can cause the distortion of the measured size distribution of the particles. Hence, the delay extraction can bring the measured distribution closer to the real one. Thus, the delay extraction technique provides not only mass resolution improvement, but also increases the hit rate. The gain in the hit rate depends on the type of the particles. It can be two orders of magnitude for model particles, and up to 2–4 times for real particles. In the present work we report experiments and results showing the effect of the delay extraction on the particles beam divergence caused by particles charge, the hit rate improvement and the effect of the delay extraction on the measured particles size distribution.

show abstract

Improvement of peaks identification and dynamic range for bi-polar Single Particle Mass Spectrometer

Cited by 10 publications

References 32 publications

Improvement of m/z accuracy for linear matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometer

Improvement of m/z accuracy for linear matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometer

Development of an automatic linear calibration method for high-resolution single-particle mass spectrometry: improved chemical species identification for atmospheric aerosols

Increase of the particle hit rate in laser single particle mass spectrometer by pulse delayed extraction technology

Contact Info

Product

Resources

About