Improvement of plastic-based disposable bioreactors for plant science needs

Werner

Engineering in Life Sciences

2009

Driven by the demands of the market and the manufacturing industry, disposable bioreactors have gained in importance in cell culture‐based processes during the last 10 years. Today they are widely accepted in R&D and also in manufacturing where process simplicity, safety and flexibility have top priority. Although disposable bioreactors are mainly used for cell expansions, glycoprotein secretions and virus generations realised with mammalian and insect cell lines, there are several reports delineating their suitability for the cultivation of plant cell and tissue cultures. This review describes the current disposable bioreactor types suitable for growing plant cell suspensions and organ cultures (hairy roots, meristematic clusters, somatic embryos) at Litre‐scale. Based on a definition of the term “disposable bioreactor”, a categorisation of the prevalent types for plant liquid cultures is presented. We describe the bioreactor regimes, working principles and bioengineering parameters of mechanically and pneumatically agitated bag bioreactors, which have advantages of process scalability and efficiency. Furthermore, results from the literature and data from our own research (obtained during production of undifferentiated bioactive cells, expressions of secondary metabolites and glycoproteins, and micropropagations of plant tissues) are discussed.

Section: Pneumatically Driven Bag Bioreactorssupporting

confidence: 71%

Disposable bioreactors for plant liquid cultures at Litre‐scale

Werner

Engineering in Life Sciences

2009

“…A técnica de regeneração de plantas por embriogênese somática está bem estabelecida para o cafeeiro, e é possível produzir mudas clonais em larga escala com uso de biorreatores de imersão temporária (Afreen et al, 2002;Ducos et al, 2007Ducos et al, , 2008. Etienne-Barry et al (1999) demonstraram ser possível semear embriões germinados, produzidos em biorreatores de imersão temporária, diretamente no substrato utilizado para a produção de mudas.…”

Section: Introductionunclassified

Características agronômicas e morfológicas de cafeeiro 'Catuaí Vermelho' propagado por embriogênese somática

Carvalho

Rezende

Almeida³

et al. 2011

Pesq. agropec. bras.

Resumo -O objetivo deste trabalho foi avaliar características agronômicas e morfológicas de plantas de Coffea arabica, cultivar Catuaí Vermelho IAC 44, propagadas por embriogênese somática. O experimento foi instalado em janeiro de 2005, em delineamento experimental de blocos ao acaso, com dez repetições. As plantas foram avaliadas mensalmente quanto ao desenvolvimento vegetativo, de junho de 2005 a janeiro de 2006, e as avaliações agronômicas foram realizadas dois anos e meio após o início do experimento. A produtividade de grãos foi avaliada durante as quatro primeiras colheitas. Cafeeiros provenientes de embriogênese somática apresentam desenvolvimento inicial mais rápido do que as plantas obtidas de sementes e, aos 30 meses após plantio no campo, têm diâmetro de copa superior ao de plantas de origem seminal. O desempenho agronômico de plantas de C. arabica produzidas por embriogênese somática é semelhante ao de plantas oriundas de sementes, e não há restrições agronômicas para a sua utilização.Termos para indexação: Coffea arabica, crescimento vegetativo, produção, propagação. Agronomic and morphological traits of 'Catuaí Vermelho' coffee plant propagated by somatic embryogenesisAbstract -The objective of this work was to assess agronomical and morphological traits of Coffea arabica plants, cultivar Catuaí Vermelho IAC 44, propagated by somatic embryogenesis. The experiment was established in January 2005 in a randomized complete block design, with 10 replicates. Plants were evaluated monthly for vegetative development, from June 2005 to January 2006, and agronomical evaluations were carried out two years and a half after the beginning of the experiment. Grain yield was evaluated during the first four harvests. Coffee plants derived from somatic embryogenesis show faster initial growth than those obtained from seeds, and had greater canopy diameter than plants with seminal origin 30 months after planting in field. The agronomic performace of C. arabica plants produced by somatic embryogenesis is similar to that of plants derived from seeds, and there are no agronomic restrictions for their use.

“…In terms of cost, cheaper sources of culture vessels and power need to be explored. The "glass jar temporary immersion bioreactor" and the "horizontal disposable temporary immersion bioreactor" (''Box-in-Bag'') were developed for purposes of cost reduction for coffea cultivation (Ducos et al, 2008). With TIBs at the heart of yam seed systems, it will be necessary to standardize the protocols for different economically important genotypes.…”

Section: Challenges Of Tibsmentioning

confidence: 99%

Status and prospects for improving yam seed systems using temporary immersion bioreactors

Balogun¹,

Maroya²,

Asiedu³

2014

Afr. J. Biotechnol.

Yam production is constrained by scarcity of clean seed, pests, diseases and low soil fertility in the informal seed system, which is still operational, causing up to 90% yield losses. Although meristem culture can be effective for producing healthy seed yam, its use is limited by slow rate of regeneration and propagation in conventional tissue cultures. In most crops tested, temporary immersion bioreactor systems (TIBs) increased propagation rates. To determine the potential of TIBs in improving the yam seed system, 23 databases were consulted and three returned a total of eight publications with only 2 for Dioscorea rotundata-cayenensis. Both plantlets and microtubers can be produced in TIBs, which will facilitate production of quality breeder, foundation and certified seeds and fast-track genetic improvement and the evolution of a formal from informal seed production system. Control of contamination, direct use of field explants, culture of micro-explants like immature embryos and anthers, increasing the size of microtubers produced and standardization for various economically important yam genotypes are knowledge gaps that require immediate research attention. No report has put a cost on yam TIBs, but it will be necessary to use cost-effective TIBs to encourage integration public-private partnerships into emerging formal seed system.