Improvement of Psidium Guajava L. Using Micropropagation

Abstract

Here we describe an innovative, reproducible, and inexpensive method for in vitro cloning of guava using nodal stems segments. A novel process was established for completely inhibiting phenol-based browning by coating the explants at their cut ends with commercial silicon. A high regeneration frequency (98.33%) was achieved on MS medium supplemented with 4 mg/L BA and 0.4 mg/L IAA, with a maximum average number of 76.17 shoots per explant produced on the third subculture. The highest rooting percentage (95.83%) was obtained on shoots growing on half strength MS medium and supplemented with 4 mg/L IAA. The plantlets were acclimatized and successfully established in soil. RAPD analysis was used to compare the regenerated plants with their donor parents: the analysis showed a high similarity (94.50% on average) among the regenerated plants and their donor parents. Taking advantage of such highly regenerative technique in a woody plant like guava, an exponential increase in the number of in vitro produced plantlets can be achieved by utilizing the resulting shoots as nodal explant starting material to commercially propagate guava in vitro.

Effect of Silicon on Somatic Embryogenesis and Shoot Regeneration of Dry Date Palm (Phoenix Dactylifera L.) Cv Bartamuda

“…Phenolic oxidative tissue browning is one of the bottlenecks in woody plant tissue culture. In guava, tissue browning was completely prevented by sealing the nodal explants cutting ends with Si (Youssef et al, 2010). Addition of Si as K 2 SiO 3 to MS medium reduced the hyperhydricity in Cotoneaster wilsonii plant by decreasing the content of MDA in the regenerated shoots when compared with the control (Sivanesan et al, 2011).…”

The role of silicon in plant tissue culture

“…Phenolic oxidative tissue browning is one of the bottlenecks in woody plant tissue culture. In guava, tissue browning was completely prevented by sealing the nodal explants cut ends with Si (Youssef et al, 2010) and there was no detrimental effect of Si on the subsequent steps of in vitro propagation. The authors suggested that Si could be used during explants preparation to control phenolic tissue browning in other plants.…”

Genetic diversity among four Eucalyptus species (myrtaceae) based on random amplified polymorphic DNA (RAPD) analysis

“…Cluster analysis was carried out on similarity estimates using the unweighted pair-group method with arithmetic average (UPGMA) software. These methods were carried out using MVSP software programs as in Youssef (2004). The results were then represented as a dendrogram for allover primers.…”