Improvement of succinate production by release of end-product inhibition in Corynebacterium glutamicum

Chung, Soon‐Chun; Park, Joon-Song; Yun, Jae Moon; Park, Jin Hwan

doi:10.1016/j.ymben.2017.02.004

Cited by 55 publications

(32 citation statements)

References 45 publications

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“…Relieving end‐product inhibition by overexpression of NCgl0275 or whcA gene, which functions as negative regulator in the oxidative stress response pathway, and increasing metabolic flux towards phosphoenolpyruvate and oxaloacetate formation by replacement of native phosphoenolpyruvate carboxylase pyruvate carboxylase by heterologous expression ppc and pckG from Mannheimia succiniciproducens under P tuf promoter allowed the highest production of succinate in fed‐batch fermentation under aerobic conditions (152.2 g L −1 ) (Fig. ; Table ) . For the efficient production of succinic acid using C. glutamicum , cells were grown in aerobic conditions and then subjected to oxygen‐deprived conditions for the conversion of glucose to succinic acid.…”

Section: Production Of Polyamide Monomersmentioning

confidence: 99%

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Baritugo

Kim

David

et al. 2018

Biofuels Bioprod Bioref

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The fermentative production of platform chemicals in biorefineries is a sustainable alternative to current petroleum‐refining processes. Industrial microorganisms, such as Escherichia coli, Saccharomyces cerevisiae, and Corynebacterium glutamicum, have been engineered as microbial cell factories that are able to utilize biomass for the production of value‐added platform chemicals and polymers. Compared to E. coli and S. cerevisiae, C. glutamicum displays weak carbon catabolite repression and can co‐utilize mixed sugars as carbon sources, without any significant growth retardation. Pathways for the utilization of alternative carbon sources, such as d‐xylose and l‐arabinose from lignocellulosic biomass, lactose and galactose from whey, glycerol from biodiesel, and methanol from natural gas refineries, have been evaluated for chemical production. However, the application of C. glutamicum in biorefineries is limited because it does not secrete hydrolases for the efficient utilization of cellulose, xylan, and starch from lignocellulosic and starch biomass. To solve the limitation, C. glutamicum has been engineered for the consolidated bioprocessing of biomass by the heterologous expression of amylolytic and cellulolytic enzymes. Recently, C. glutamicum has been extensively engineered for polyamide monomer production owing to its ability to produce l‐lysine and l‐glutamate. This review summarizes recent advances in the development of C. glutamicum strains that can utilize renewable biomass resources for the production of industrially important chemicals. It highlights recent progress in metabolic engineering for the production of polyamide monomers. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

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Section: Production Of Polyamide Monomersmentioning

confidence: 99%

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Baritugo

Kim

David

et al. 2018

Biofuels Bioprod Bioref

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“…Many previous researches have focus on producing succinate from renewable resources (e.g., glucose, xylose, glycerol, starch etc.) by different microorganism (Choi et al, ; Chung, Park, Yun, & Park, ; Li, Huang, et al, ; Lin, Bennett, & San, ; Wu, Li, Zhou, & Ye, ).…”

Section: Introductionmentioning

confidence: 99%

Central pathway engineering for enhanced succinate biosynthesis from acetate in Escherichia coli

Huang

Yang

Fang

et al. 2018

Biotech & Bioengineering

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Acetate, a non-food based substrate obtained from multiple biological and chemical ways, is now being paid great attention in bio-manufacturing and have a strong potential to compete with sugar-based carbon source. In this study, acetate can be efficiently converted to succinate by engineered Escherichia coli strains via the combination of several metabolic engineering strategies, including reducing OAA decarboxylation, engineering TCA cycle, enhancement of acetate assimilation pathway and increasing aerobic ATP supply through cofactor engineering. The engineered strain HB03(pTrc99a-gltA, pBAD33-Trc-fdh) accumulated 30.9 mM of succinate in 72 hr and the yield reached the maximum theoretical yield (∼0.50 mol/mol). In the resting-cell experiments, the yield of succinate in HB03(pTrc99a-gltA) and HB03(pTrc99a-gltA, pBAD33-Trc-fdh) dropped dramatically, although the productivity of succinate increased due to the high cell density. Further deletion of icdA, formed HB04(pTrc99a-gltA) and HB04(pTrc99a-gltA, pBAD33-Trc-fdh), increased the yield of succinate in the resting-cell experiments. The highest concentration of succinate achieved 194 mM and the yield reached 0.44 mol/mol in 16 hr by HB04(pTrc99a-gltA, pBAD33-Trc-fdh). The results showed the metabolically engineered E. coli strains have great potential to produce succinate from acetate.

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“…More than 628 mM (74 g/L) succinate was produced from hydrolysate with a nominal yield of 1.76 mol/mol (1.15 g/g) in a modified fed‐batch fermentation (Figure c). Although a higher titer (152.2 g/L succinic acid from glucose in Corynebacterium glutamicum ; Chung et al, ) and higher productivity (3.49 g/L/hr succinate from glucose and glycerol in Mannheimia succiniciproducens ; Choi et al, ) was obtained from pure carbon source (glucose with/without glycerol), this is the highest succinate titer reported to date in E. coli from galactose‐rich hydrolysate when the succinate yield is higher than 1 g/g (high titer and high yield) as far as we know (Jiang et al, ). It is worth to note that succinate productivity dropped as the succinate accumulated, which makes the overall productivity drop to 1.20 g/L/hr in modified fed‐batch fermentation using hydrolysate as a carbon source.…”

Section: Discussionmentioning

confidence: 96%

“…Recently, many strains have been screened and engineered for succinic acid production based on three pathways (Ahn, Jang, & Yup Lee, ). Many remarkable breakthroughs have been achieved, for example, more than 110 g/L succinic acid has been produced from glucose in E. coli (Jiang et al, ), up to 152.2 g/L succinic acid was accumulated using glucose as carbon source in Corynebacterium glutamicum (Chung, Park, Yun, & Park, ), 90.68 g/L succinic acid was produced with an overall productivity of 3.49 g/L/hr in a chemically defined medium based fed‐batch fermentation using glucose and glycerol as carbon sources in Mannheimia succiniciproducens (Choi et al, ), and several industrial plants using engineered strains to produce succinic acid have been reported (Ahn, Jang, & Lee, ). However, bio‐based succinic acid still faces a challenge in competition against petrochemical‐based succinic acid due to the high production and purification cost (McKinlay, Vieille, & Zeikus, ).…”

Section: Introductionmentioning

confidence: 99%

Improved succinate production from galactose‐rich feedstocks by engineered Escherichia coli under anaerobic conditions

Zhu

San

Bennett

2020

Biotech & Bioengineering

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It is of great economic interest to produce succinate from low‐grade carbon sources, which can make it more economically competitive against petrochemical‐based succinate. Galactose sugars constitute a significant fraction of the soluble carbohydrate in a meal from agricultural sources which is considered a low value or waste byproduct of oilseed processing. To improve the galactose utilization, the effect of galR and glk on sugars uptake was investigated by deactivation of each gene in three previously engineered host strains. As expected, glk plays an important role in glucose uptake, while, the effect of deactivation of galR is highly dependent on the strength of the downstream module (succinate production module). A new succinate producer FZ661T was constructed by enhancement of the succinate producing module and manipulation of the gal operon. The succinate productivity reached 4.57 g/L/hr when a mixed sugar feedstock was used as a carbon source in shake‐flask fermentation, up to 812 mM succinate was accumulated in 80 hr in fed‐batch fermentation. When SoyMolaGal hydrolysate was used as a carbon source, 628 mM (74 g/L) succinate was produced within 72 hr. In this study, we demonstrate that FZ661T can produce succinate quickly with relatively high yield, giving it the potential for industrial application.

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Improvement of succinate production by release of end-product inhibition in Corynebacterium glutamicum

Cited by 55 publications

References 45 publications

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Central pathway engineering for enhanced succinate biosynthesis from acetate in Escherichia coli

Improved succinate production from galactose‐rich feedstocks by engineered Escherichia coli under anaerobic conditions

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