Improvement of the application of gonadal tissue allotransplantation in the in vitro conservation of chicken genetic lines

Liptói, Krisztina; Buda, Kitti; Rohn, Emese; Drobnyák, Árpád; Meleg, Erika Edvine; Palinkas-Bodzsar, Nora; Végi, Barbara; Barna, Judit

doi:10.1016/j.anireprosci.2020.106280

Cited by 17 publications

(33 citation statements)

References 13 publications

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“…With the abdominal air sac membrane left intact, the view of the ovary was obstructed making it di cult to avoid blood vessel damage, and thus resulting in higher mortality. Unfortunately, a majority of studies in other avian species do not report surgery survival rates, making comparison with chicken, quail, and duck studies di cult [6,7,9,12,14]. Furthermore, out of the studies which did report survival rates, no differentiation was made between deaths on the operating table or deaths during the recovery period [5,10,11].…”

Section: Discussionmentioning

confidence: 99%

“…Poultry semen cryopreservation techniques are quite advanced and have been researched extensively [4]. In contrast, cryopreservation and transplantation of poultry ovarian tissue is documented in only a handful of studies [5][6][7]. Presently, the limitation remains the low transplantation success rates, even when fresh tissue is used, which has reduced the widespread adoption of ovarian biobanking.…”

Section: Introductionmentioning

confidence: 99%

“…However, in most studies a majority of hens also produced recipient-derived progeny making them gonadal chimeras. As recipientderived progeny are a waste of nancial resources, reducing their occurrence by eliminating gonadal chimeras has been previously attempted through removal of the entire recipient ovary either by using surgical forceps [9] or via cauterization [7]. The sterilization of recipients with busulfan before ovary removal and transplantation was also reported [10].…”

Section: Introductionmentioning

confidence: 99%

“…The sterilization of recipients with busulfan before ovary removal and transplantation was also reported [10]. In chickens, the removal of the entire ovary alone, either with forceps or cauterization, still resulted in 6 out of 7 (86%) hens, being gonadal chimeras [7,9,10]. When hens were sterilized with busulfan prior to the removal of the entire recipient's ovary, gonadal chimeras still developed.…”

Section: Introductionmentioning

confidence: 99%

“…Since primordial germ cell migration occurs only during embryonic development in poultry, it is unlikely that the donor ovarian tissue was repopulated by recipient primordial germ cells, but rather, the recipient's ovary was not entirely removed leaving donor germ cells in situ [7]. Therefore, improving upon current techniques of removal, might lower the number of gonadal chimeras generated.…”

Section: Introductionmentioning

confidence: 99%

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Turkey Ovarian Tissue Transplantation: Effects of Surgical Technique on Graft Attachment and Immunological Status of the Grafts 6 Days Post-surgery

Hall

Long

Susta

et al. 2021

Preprint

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Background: Biobanked poultry ovaries can be revived via transplantation, into a recipient female, which upon maturity will produce donor-derived progeny. Previously, a large portion of these recipients also produced recipient-derived progeny, making them gonadal chimeras. These were potentially created when portions of the recipient’s ovary were inadvertently left behind. Completely removing the recipient ovary would solve this problem, however, leaving a portion of the recipient’s ovary may have inadvertently increased the transplant attachment rate by providing a damaged area for the transplant to attach too. To test this hypothesis in the turkey, we removed various portions (33% to 100%) of recipient ovarian tissue and determined the transplant attachment rate. Furthermore, the use of the abdominal air sac membrane as an additional anchoring point was tested. Finally, the immunological status of the grafts was evaluated by analyzing the presences of CD3 and MUM-1 (T and B cell markers), 6 days post-surgery.Results: The overall attachment rate of transplants was 91% (32/35), while the average size of the transplants was 4.2 ± 0.6 mm2. There was no difference (P > 0.05) in the attachment rates, or transplant size between groups with varying amounts of recipent tissue removed, or by using the abdominal air sac membrane as an anchor. However, all transplants were infiltrated by large numbers of T and B cells. This was shown by a high (P ≤ 0.001) percentage of CD3-positive immunostained cytoplasmic area (49.78 ± 3.90%) in transplants compared to remnant recipient tissue (0.30 ± 0.10%), as well as a high (P ≤ 0.001) percentage of MUM-1-positive immunostained nuclear area (9.85 ± 1.95%) in transplants over remnant recipient tissues (0.39 ± 0.12%).Conclusions: This study showed that neither the portion of the recipient ovary left behind nor the use of the abdominal air sac membrane affected the rate of attachment or the amount of donor tissue that attached. Thus, we recommend removing the entire recipient ovary to prevent gonadal chimeras. The high levels of lymphocytes within the grafts indicate possible tissue rejection, which could be overcome via immunosuppression with or without histocompatibility matching between donors and recipients.

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