Improvement of the grapes propagation technology in vitro

Abstract

Abstract. The aim of research is improvement of the production technology in vitro of the grapes Pamyati Dombkovskoy. Methods. Methods generally accepted in the practice of clonal micropropagation of plants were applied. There are sterilization of the starting material, introduction into culture, clonal micropropagation, and in vitro rooting, followed by adaptation to in vivo conditions. The study object was micro cuttings grapes of the cultural variety Pamyati Dombkovskoy. The experiments were set up in three replicates, one replication was at least 10 test tubes. Statistical processing of the data obtained was carried out by the dispersion method according to B. A. Dospekhov. The following parameters were taken into account: micro-shoots and microplants heights, leaves number, proliferation coefficient. Root development was assessed in points. The success of adaptation was considered as the percentage of adapted microplants to the total number planted in the substrate. At the stage of adaptation, we applied supplemented and developed by us technique during clonal micropropagation of the Angelica rose. Research result. Success of estanlishment explants was 40 % on Murashige and Skoog growing medium with a reduced content of macronutrients, when introduced into a sterile culture in vitro. The optimal concentration of 6-benzylaminopurine (6-BAP) was determined at the proliferation stage – 1 mg/l. There are decrease in the proliferation coefficient from 4.4 pcs/cutting to 3.3 and 2.9 pcs/cutting respectively with an increase in the concentration of cytokinin 6-BAP to 2.0 and 3.0 mg / l (LSD05 = 1.0). It was revealed that the best growing medium for rooting micrograpes is the environment according to Zlenko and others, when the best microplants development is achieved according to such morphometric parameters as microshoots height, leaves number and root system. On the medium Zlenko and others, microplants height was higher by 4.3 mm than the control with LSD05 = 2.7, the number of leaves was more by 0.5 with LSD05 = 0.3, and the root system of microplants is better developed by 0.4 points (LSD05 = 0.2). Scientific novelty. Positive results were received when rooted grapes cuttings were adapted after 14 days of cultivation on rooting medium, which allows to reduce the duration of micro grapes in a test tube in 2–4 times compared with the conventional method. The use of the biological product “Trichoderma Veride” for watering the soil substrate with the subsequent spraying of adaptable microplants with the organosilicone fertilizer “Siliplant” is recommended.