Abstract. The aim of research is improvement of the production technology in vitro of the grapes Pamyati Dombkovskoy. Methods. Methods generally accepted in the practice of clonal micropropagation of plants were applied. There are sterilization of the starting material, introduction into culture, clonal micropropagation, and in vitro rooting, followed by adaptation to in vivo conditions. The study object was micro cuttings grapes of the cultural variety Pamyati Dombkovskoy. The experiments were set up in three replicates, one replication was at least 10 test tubes. Statistical processing of the data obtained was carried out by the dispersion method according to B. A. Dospekhov. The following parameters were taken into account: micro-shoots and microplants heights, leaves number, proliferation coefficient. Root development was assessed in points. The success of adaptation was considered as the percentage of adapted microplants to the total number planted in the substrate. At the stage of adaptation, we applied supplemented and developed by us technique during clonal micropropagation of the Angelica rose. Research result. Success of estanlishment explants was 40 % on Murashige and Skoog growing medium with a reduced content of macronutrients, when introduced into a sterile culture in vitro. The optimal concentration of 6-benzylaminopurine (6-BAP) was determined at the proliferation stage – 1 mg/l. There are decrease in the proliferation coefficient from 4.4 pcs/cutting to 3.3 and 2.9 pcs/cutting respectively with an increase in the concentration of cytokinin 6-BAP to 2.0 and 3.0 mg / l (LSD05 = 1.0). It was revealed that the best growing medium for rooting micrograpes is the environment according to Zlenko and others, when the best microplants development is achieved according to such morphometric parameters as microshoots height, leaves number and root system. On the medium Zlenko and others, microplants height was higher by 4.3 mm than the control with LSD05 = 2.7, the number of leaves was more by 0.5 with LSD05 = 0.3, and the root system of microplants is better developed by 0.4 points (LSD05 = 0.2). Scientific novelty. Positive results were received when rooted grapes cuttings were adapted after 14 days of cultivation on rooting medium, which allows to reduce the duration of micro grapes in a test tube in 2–4 times compared with the conventional method. The use of the biological product “Trichoderma Veride” for watering the soil substrate with the subsequent spraying of adaptable microplants with the organosilicone fertilizer “Siliplant” is recommended.

Abstract. The purpose of the study is to develop a technology for the production of stevia planting material based on the method of clonal micropropagation and the use of silicon oxide at the stage of adaptation, evaluation of the effectiveness of the influence of silicon oxide in the adaptation of microplants. Methods. Commonly used in the practice of clonal micropropagation of plants were applied. Methods generally accepted in the practice of clonal micropropagation of plants were applied: sterilization of the initial material, introduction into culture in vitro, clonal micropropagation proper, rooting with subsequent adaptation to environmental conditions. The object of the study was stevia: at the stage of introduction into a sterile culture - seeds, at subsequent stages - microcuttings and microplants. At the adaptation stage, silicon oxide was used in the form of a solution of orthophosphoric acid at a concentration of 0.01%. Scientific novelty. The possibility of obtaining microplants at the stage of micropropagation itself, bypassing the stage of rooting, is shown. On the MS medium with epin 0.1 mg/l in combination with indolyl-3-acetic acid (IAA) 0.5 mg/l, high-quality plants of small sizes develop: shoot length on average 5.6 cm, well-formed unfolded leaves and root system, relatively short internodes. Research results. At the stage of introduction into a sterile culture during sterilization with a 33 % hydrogen peroxide solution in an exposure of 8–10 minutes, a high infection of seeds (about 70 %) and their low germination (10 %) were noted, which can be explained by the structure of the seeds and the biology of the species. At the stage of micropropagation itself, the maximum effect was obtained when MS 6-BAP was included in the nutrient medium at a dose of 0.5 mg/l. The combined inclusion of epin growth stimulants with IAA and IMA in the composition of the media contributed to the development of roots by 2.1 points and 1.8 points, respectively, which was at the control level – 1.8 points. On media with cytokinins, the roots were weakly expressed (0.3 points with 6-BAP) or absent (with kinetin), which was a significantly worse result compared to the control. The output of adapted microdenies in the spring amounted to 80 %. However, during the summer landing for adaptation, a decrease in the survival rate of plants was observed. In order to increase the efficiency of adaptation, we used 0.01 % silicon oxide.

Abstract. The purpose of the study was to test the effect of an aqueous solution of silicon oxide on the sowing qualities of plant seeds. Methods. The assessment of the effect of silicon oxide (SiO2) on the sowing qualities of seeds was carried out by a laboratory method according to Medical and biological safety assessment of nanomaterials 1.2.2635-10 and GOST 12038-84 “Seeds of agricultural crops. Methods for determining germination”. Asparagus bean seeds Vigna unguiculata subsp. sesquipedalis of Matilda variety, spring wheat Triticum aestivum L. of Svecha variety, seed radish Raphanus sativus L. of Champion variety and onion Allium cepa L. of Odintsovo variety. The following indicators were taken into account: germination energy and seed germination, number, length and weight of underground and aboveground parts of seedlings. Statistical data processing was carried out by the dispersion method according to B. A. Dospekhov. Scientific novelty. The species-specific reaction of seeds to the treatment with silicon oxide was revealed. The best results in terms of morphometric parameters of seedlings of asparagus bean and spring wheat were obtained when seeds were treated with 0.0025 % silicon oxide, radish – 0.005 %. It is not advisable to treat onion seeds with silicon oxide. According to the research results, asparagus bean seeds turned out to be the most responsive to processing. Germination energy and seed germination in the variant of treatment with 0.0025 % silicon oxide compared to the control (distilled water) were 13.7 % and 3.0 % higher, respectively, but the difference is not significant. The studied concentrations contributed to a significant increase in the average length of the roots. At 0.01 % concentration, this indicator was higher by 30.6 mm, 0.005 % by 30.7 mm, at 0.0025 % by 48.8 mm, respectively (HCP05 = 30.1). The average root weight was 67.5 mg more in the variant of seed treatment with 0.0025% silicon oxide solution (HCP05 = 41.5).