Improvement of the stability and selectivity of Rhizomucor miehei lipase immobilized on silica nanoparticles: Selective hydrolysis of fish oil using immobilized preparations

Mohammadi, Mehdi; Habibi, Zohreh; Dezvarei, Shaghayegh; Yousefi, Maryam; Samadi, Saadi; Ashjari, Maryam

doi:10.1016/j.procbio.2014.04.012

Cited by 46 publications

(27 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The surface was then reacted directly with lipases as described in materials and methods. thoroughly with CHCl 3 and dried at 60 • C for 2 h. The presence of epoxy groups on the support was confirmed in our previous study by IR spectroscopy and TGA-DTA analysis [34]. Quantification of the epoxy groups on the support was carried out as follows: 200 mg of the support was added to 1.5 ml of 1.3 M sodium thiosulphate solution and the solution was titrated by addition of 0.1 M hydrochloric acid until neutralization.…”

Section: Functionalization Of Silica Particlesmentioning

confidence: 91%

“…Dried silica gel was reacted with 3-GPTMS in presence of Et 3 N to give silica-epoxy support. Previously, we developed immobilization of RML on this support for hydrolysis of fish oil [34]. The epoxide group can normally undergo a reaction across a wide range of pH values from acidic to basic but the most efficient ring opening is obtained at the extreme of pH [32].…”

Section: Synthesis and Characterization Of Immobilized Lipasesmentioning

confidence: 99%

“…Thermal stability of RML-epoxy derivative has been investigated previously [34]. Free enzyme showed optimum activity at 45 • C and a drastic loss in activity on further increase in temperature.…”

Section: Thermal Stabilitymentioning

confidence: 99%

“…During our development of lipase immobilization techniques for organic synthesis applications [33][34][35][36], we have investigated silica gel surface treatments with respect to the immobilization efficiency of lipases as well as applicability of that treatment for use in biodiesel synthesis. This paper describes the modification of silica surfaces and immobilization of three lipases Candida antarctica lipase B (CALB), Thermomyces lanuginosus lipase (TLL) and Rhizomucor miehei lipase (RML) onto epoxy functionalized silica.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Preparation of highly reusable biocatalysts by immobilization of lipases on epoxy-functionalized silica for production of biodiesel from canola oil

Babaki

Yousefi

Habibi

et al. 2015

Biochemical Engineering Journal

Self Cite

View full text Add to dashboard Cite

Section: Functionalization Of Silica Particlesmentioning

confidence: 91%

Section: Synthesis and Characterization Of Immobilized Lipasesmentioning

confidence: 99%

Section: Thermal Stabilitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Preparation of highly reusable biocatalysts by immobilization of lipases on epoxy-functionalized silica for production of biodiesel from canola oil

Babaki

Yousefi

Habibi

et al. 2015

Biochemical Engineering Journal

Self Cite

View full text Add to dashboard Cite

“…[ [40][41][42][43][44][45][46][47][48][49][50][51][52][53][54] Extracellular expression of recombinant SpA…”

Section: Spa Production Methodsmentioning

confidence: 99%

A comprehensive review on staphylococcal protein A (SpA): Its production and applications

Rigi

Ghaedmohammadi

Ahmadian

2019

Biotech and App Biochem

View full text Add to dashboard Cite

The Staphylococcus aureus protein A (SpA) can be obtained through the culture of wild-type S. aureus and also as a recombinant protein in safe bacterial hosts. Several methods have been used to purify SpA among which ion-exchange chromatography, affinity chromatography, gel filtration, and per aqueous liquid chromatography (PALC) are common. SpA has a wide range of biochemical, biotechnological, and medical applications and is most commonly used in test methods such as immunoprecipitation, enzyme-linked immunosorbent assay, and Western blotting. SpA has also been widely utilized in pharmaceutical applications to bind to immune complexes and serum immunoglobulins. SpA also directly binds to the B-cells preventing initiation of infectious diseases as well as having a role in the development of various autoimmune diseases. This review considers different applications of SpA in biotechnology and its novel clinical application for effective treatment of autoimmune diseases. It also discusses various strategies for expression and purification of the SpA including types of column chromatography that are commonly used in protein purification and developing SpA surface display technologies. Finally, this review highlights the potential and novel applications of SpA immobilization, SpA typing, protein engineering for further development of immunological and biochemical research, and also application of SpA as a diagnostic biosensor.

show abstract

Liquid lipase‐catalyzed hydrolysis of gac oil for fatty acid production: Optimization using response surface methodology

Nguyen

et al. 2018

Biotechnology Progress

View full text Add to dashboard Cite

Fatty acids are valuable products because they have wide industrial applications in the manufacture of detergents, cosmetics, food, and various biomedical applications. In enzyme‐catalyzed hydrolysis, the use of immobilized lipase results in high production cost. To address this problem, Eversa Transform lipase, a new and low‐cost liquid lipase formulation, was used for the first time in oil hydrolysis with gac oil as a triglyceride source in this study. Response surface methodology was employed to optimize the reaction conditions and establish a reliable mathematical model for predicting hydrolysis yield. A maximal yield of 94.16% was obtained at a water‐to‐oil molar ratio of 12.79:1, reaction temperature of 38.9 °C, enzyme loading of 13.88%, and reaction time of 8.41 h. Under this optimal reaction condition, Eversa Transform lipase could be reused for up to eight cycles without significant loss in enzyme activity. This study indicates that the use of liquid Eversa Transform lipase in enzyme‐catalyzed oil hydrolysis could be a promising and cheap method of fatty acid production. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 2018

show abstract

Improvement of the stability and selectivity of Rhizomucor miehei lipase immobilized on silica nanoparticles: Selective hydrolysis of fish oil using immobilized preparations

Cited by 46 publications

References 38 publications

Preparation of highly reusable biocatalysts by immobilization of lipases on epoxy-functionalized silica for production of biodiesel from canola oil

Preparation of highly reusable biocatalysts by immobilization of lipases on epoxy-functionalized silica for production of biodiesel from canola oil

A comprehensive review on staphylococcal protein A (SpA): Its production and applications

Liquid lipase‐catalyzed hydrolysis of gac oil for fatty acid production: Optimization using response surface methodology

Contact Info

Product

Resources

About