Preparation of highly reusable biocatalysts by immobilization of lipases on epoxy-functionalized silica for production of biodiesel from canola oil

Babaki, Mohadese; Yousefi, Maryam; Habibi, Zohreh; Brask, Jesper; Mohammadi, Mehdi

doi:10.1016/j.bej.2015.04.020

Cited by 87 publications

(49 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Typical strategies for immobilizing enzymes onto mesoporous silicas rely on surface grafting via polymers containing functional groups or low molecular weight linkers to which enzymes are reacted by covalent conjugation101112. But such immobilization methods are usually accompanied by enzymes deactivation13.…”

mentioning

confidence: 99%

Dopamine-functionalized mesoporous onion-like silica as a new matrix for immobilization of lipase Candida sp. 99-125

Gao

Jiang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Dopmine functionalized mesoporous onion-like silica (DPMS) was synthesized via a biomimetic coating, and lipase Candida sp. 99-125 (LCS) was immobilized in DPMS (LCS@DPMS) by physical adsorption in this study. The DPMS was characterized by SEM, TEM, BET and FT-IR, and it was shown that the DPMS had clear multishell structures with large surface area of 419 m2/g. The activity, pH stability, thermal stability, storage stability, and reusability of the LCS@DPMS were investigated in detail. The stabilities of LCS@DPMS were improved significantly compared to the free lipase and LCS@MS (LCS immobilized in unfunctionalized mesoporous onion-like silica by physical adsorption). All the results indicated that the DPMS had high efficiency and improved stability for lipase immobilization.

show abstract

mentioning

confidence: 99%

Dopamine-functionalized mesoporous onion-like silica as a new matrix for immobilization of lipase Candida sp. 99-125

Gao

Jiang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…[ [40][41][42][43][44][45][46][47][48][49][50][51][52][53][54] Extracellular expression of recombinant SpA…”

Section: Spa Production Methodsmentioning

confidence: 99%

“…[14][15][16][39][40][41][42][43][44] with MabSelect [31]. Some cellular proteins contain two or more adjacent histidine residues which may interfere with affinity purification.…”

Section: Per Aqueous Liquid Chromatography (Palc)mentioning

confidence: 99%

“…Immobilization of proteins on a solid support is an efficient way to make these processes more cost effective as the immobilized proteins can be easily recovered and used in the continuous rounds of the reaction [40]. There are various immobilization methods such as: adsorption [41], covalent bonding [42], entrapment [43], polymerization [44], and encapsulation [45].…”

Section: Site-specific Immobilization Of Spa On Nanoparticles For Onementioning

confidence: 99%

See 1 more Smart Citation

A comprehensive review on staphylococcal protein A (SpA): Its production and applications

Rigi

Ghaedmohammadi

Ahmadian

2019

Biotech and App Biochem

View full text Add to dashboard Cite

The Staphylococcus aureus protein A (SpA) can be obtained through the culture of wild-type S. aureus and also as a recombinant protein in safe bacterial hosts. Several methods have been used to purify SpA among which ion-exchange chromatography, affinity chromatography, gel filtration, and per aqueous liquid chromatography (PALC) are common. SpA has a wide range of biochemical, biotechnological, and medical applications and is most commonly used in test methods such as immunoprecipitation, enzyme-linked immunosorbent assay, and Western blotting. SpA has also been widely utilized in pharmaceutical applications to bind to immune complexes and serum immunoglobulins. SpA also directly binds to the B-cells preventing initiation of infectious diseases as well as having a role in the development of various autoimmune diseases. This review considers different applications of SpA in biotechnology and its novel clinical application for effective treatment of autoimmune diseases. It also discusses various strategies for expression and purification of the SpA including types of column chromatography that are commonly used in protein purification and developing SpA surface display technologies. Finally, this review highlights the potential and novel applications of SpA immobilization, SpA typing, protein engineering for further development of immunological and biochemical research, and also application of SpA as a diagnostic biosensor.

show abstract

“…Moreover, it has problems of glycerol recovery and wastewater treatment [2][3][4][5]. Enzymatic transesterification using lipase is becoming more attractive due to its easier recovery of glycerol, mild reaction conditions, minimal requirement of wastewater treatment and wide adaptability for feedstock [6][7][8][9][10][11]. However, extracellular lipases as biocatalysts require complicated purification, recovery, and immobilization.…”

Section: Introductionmentioning

confidence: 99%

Biodiesel production using magnetic whole-cell biocatalysts by immobilization of Pseudomonas mendocina on Fe3O4-chitosan microspheres

Chen

Liu

et al. 2016

Biochemical Engineering Journal

View full text Add to dashboard Cite

Preparation of highly reusable biocatalysts by immobilization of lipases on epoxy-functionalized silica for production of biodiesel from canola oil

Cited by 87 publications

References 45 publications

Dopamine-functionalized mesoporous onion-like silica as a new matrix for immobilization of lipase Candida sp. 99-125

Dopamine-functionalized mesoporous onion-like silica as a new matrix for immobilization of lipase Candida sp. 99-125

A comprehensive review on staphylococcal protein A (SpA): Its production and applications

Biodiesel production using magnetic whole-cell biocatalysts by immobilization of Pseudomonas mendocina on Fe3O4-chitosan microspheres

Contact Info

Product

Resources

About